Developments in spontaneous and coherent Raman scattering microscopic imaging for biomedical applications

Krafft, Christoph; Schie, Iwan W.; Meyer, Tobias; Schmitt, Michael; Popp, Jürgen

doi:10.1039/c5cs00564g

Cited by 163 publications

(138 citation statements)

References 237 publications

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“…Coherent Raman scattering (CRS) [39] is a powerful tool for label-free identification of molecules in cells and tissues, based on their intrinsic vibrational response, with growing applications in biomedical optics [40]. Among CRS techniques, SRS is emerging as the one of choice due to advantages, such as the linear concentration dependence of the signal, and the lack of non-resonant background [41].…”

Section: B2 Broadband Stimulated Raman Scattering Microscopymentioning

confidence: 99%

Linear and Nonlinear Spectroscopy by a Common-Path Birefringent Interferometer

Preda

Oriana

Réhault

et al. 2017

IEEE J. Select. Topics Quantum Electron.

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We introduce a passive common-path interferometer to replace Michelson interferometers in Fourier transform spectroscopy. Our device exploits birefringence to introduce a highly accurate delay between two orthogonal polarization components by continuously varying the material thickness. Thanks to its inherent delay stability and reproducibility, it can be used even for short wavelengths (down to ~200nm) without the need for any active control or position tracking. We first demonstrate its performances in linear spectroscopy, by implementing a spectrometer and a spectrophotometer. We then extend its use to nonlinear spectroscopy and, in combination with lock-in detection at MHz modulation frequencies, illustrate its application to pump-probe spectroscopy with high sensitivity (ΔT⁄T<3·10-6 in 1-s integration time) and broad spectral coverage (>500nm) and to broadband stimulated Raman scattering microscopy in the CH stretching\ud region

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Section: B2 Broadband Stimulated Raman Scattering Microscopymentioning

confidence: 99%

Linear and Nonlinear Spectroscopy by a Common-Path Birefringent Interferometer

Preda

Oriana

Réhault

et al. 2017

IEEE J. Select. Topics Quantum Electron.

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“…As an emerging multiphoton optical imaging technique, stimulated Raman scattering (SRS) microscopy is sensitive and specific in detecting chemical bonds, offering diffraction-limited subcellular resolution, linear concentration dependence with background-free chemical contrast for quantitative measurement and intrinsic 3D optical sectioning capability1516171819202122. The use of picosecond excitation pulses and near-infrared wavelength also reduce photon scattering inside tissue samples and potential phototoxicity232425.…”

mentioning

confidence: 99%

Bioorthogonal chemical imaging of metabolic activities in live mammalian hippocampal tissues with stimulated Raman scattering

Lamprecht

et al. 2016

Sci Rep

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Brain is an immensely complex system displaying dynamic and heterogeneous metabolic activities. Visualizing cellular metabolism of nucleic acids, proteins, and lipids in brain with chemical specificity has been a long-standing challenge. Recent development in metabolic labeling of small biomolecules allows the study of these metabolisms at the global level. However, these techniques generally require nonphysiological sample preparation for either destructive mass spectrometry imaging or secondary labeling with relatively bulky fluorescent labels. In this study, we have demonstrated bioorthogonal chemical imaging of DNA, RNA, protein and lipid metabolism in live rat brain hippocampal tissues by coupling stimulated Raman scattering microscopy with integrated deuterium and alkyne labeling. Heterogeneous metabolic incorporations for different molecular species and neurogenesis with newly-incorporated DNA were observed in the dentate gyrus of hippocampus at the single cell level. We further applied this platform to study metabolic responses to traumatic brain injury in hippocampal slice cultures, and observed marked upregulation of protein and lipid metabolism particularly in the hilus region of the hippocampus within days of mechanical injury. Thus, our method paves the way for the study of complex metabolic profiles in live brain tissue under both physiological and pathological conditions with single-cell resolution and minimal perturbation.

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“…Coherent Raman imaging, such as stimulated Raman scattering or coherent anti-Stokes Raman scattering, presents a sensitivity of detection that is impressively enhanced for discrete spectral features that must be predetermined as diagnostic markers. 147–150 Such technological developments pave the way for high-throughput, rapid collection of large datasets accessible from tissue microarray tools.…”

Section: Vibrational Imaging: a Powerful Emerging Toolfor Scientists mentioning

confidence: 99%