Evidences of high carbon catabolic enzyme activities during sporulation of <i>Pleurotus ostreatus</i> (Florida)

Chakraborty, Tapas Kumar; Das, Nirmalendu; Mukherjee, M.

doi:10.1002/jobm.200310275

Cited by 12 publications

(15 citation statements)

References 21 publications

(14 reference statements)

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“…Good utilization of citric acid cycle (TCA cycle) intermediates and their metabolic derivatives such as˛-keto-glutaric acid, malic acid, fumaric acid, succinic acid, glutamic acid, ornithin, proline, -amino-butyric acid, threonine and aspartic acid, suggests a highly active TCA cycle during the growth phase. These results agree with those reported by Chakraborty et al [28] that glycolysis and TCA cycles are the major route of carbohydrate catabolism during fruitbody development. Enzymes of pentose phosphate pathway (PPP) always showed lower specific activities as compared to those of the enzymes of the glycolytic and TCA pathways.…”

Section: Substrate Assimilation Fingerprint Of P Ostreatussupporting

confidence: 96%

Optimization of biomass production with enhanced glucan and dietary fibres content by Pleurotus ostreatus ATHUM 4438 under submerged culture

Papaspyridi¹,

Katapodis²,

Gonou-Zagou³

et al. 2010

Biochemical Engineering Journal

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Section: Substrate Assimilation Fingerprint Of P Ostreatussupporting

confidence: 96%

Optimization of biomass production with enhanced glucan and dietary fibres content by Pleurotus ostreatus ATHUM 4438 under submerged culture

Papaspyridi¹,

Katapodis²,

Gonou-Zagou³

et al. 2010

Biochemical Engineering Journal

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“…These results suggest that enolase may play an important role during fruiting body formation and development of Cordyceps . It is consistent with that glycolysis and TCA cycles are the major pathways of glycometabolism in sporulating stage of fruiting body development in Pleurotus ostreatus (Chakraborty et al 2003). In contrast to C. sinensis , malate dehydrogenase showed lower expression in the fruiting body of C. memorabilis and mature one of C. militaris , which may attribute to the different formation mechanisms of individual fungus because the fruiting body of both C. memorabilis and C. militaris could be produced under the same culture conditions, but C. sinensis failed to develop its fruiting body.…”

Section: Proteins Involved In Carbohydrate Metabolismsupporting

confidence: 88%

Potential molecular mechanisms for fruiting body formation of Cordyceps illustrated in the case ofCordyceps sinensis

et al. 2017

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The fruiting body formation mechanisms of Cordyceps sinensis are still unclear. To explore the mechanisms, proteins potentially related to the fruiting body formation, proteins from fruiting bodies, and mycelia of Cordyceps species were assessed by using two-dimensional fluorescence difference gel electrophoresis, and the differential expression proteins were identified by matrix-assisted laser desorption/ionisation tandem time of flight mass spectrometry. The results showed that 198 differential expression proteins (252 protein spots) were identified during the fruiting body formation of Cordyceps species, and 24 of them involved in fruiting body development in both C. sinensis and other microorganisms. Especially, enolase and malate dehydrogenase were first found to play an important role in fruiting body development in macro-fungus. The results implied that cAMP signal pathway involved in fruiting body development of C. sinensis, meanwhile glycometabolism, protein metabolism, energy metabolism, and cell reconstruction were more active during fruiting body development. It has become evident that fruiting body formation of C. sinensis is a highly complex differentiation process and requires precise integration of a number of fundamental biological processes. Although the fruiting body formation mechanisms for all these activities remain to be further elucidated, the possible mechanism provides insights into the culture of C. sinensis.

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“…Glycolysis and TCA cycles were considered to be the major routes of carbohydrate catabolism during fruiting body development in P. ostreatus [98] and L. bicolor [88]. Glycolysis is a central pathway that produces important precursor metabolites including glucose-6-phosphate and pyruvate [99].…”

Section: Resultsmentioning

confidence: 99%

Transcriptome and Proteome Exploration to Provide a Resource for the Study of Agrocybe aegerita

Wang

Huang

et al. 2013

PLoS ONE

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Background Agrocybe aegerita, the black poplar mushroom, has been highly valued as a functional food for its medicinal and nutritional benefits. Several bioactive extracts from A. aegerita have been found to exhibit antitumor and antioxidant activities. However, limited genetic resources for A. aegerita have hindered exploration of this species.Methodology/Principal FindingsTo facilitate the research on A. aegerita, we established a deep survey of the transcriptome and proteome of this mushroom. We applied high-throughput sequencing technology (Illumina) to sequence A. aegerita transcriptomes from mycelium and fruiting body. The raw clean reads were de novo assembled into a total of 36,134 expressed sequences tags (ESTs) with an average length of 663 bp. These ESTs were annotated and classified according to Gene Ontology (GO), Clusters of Orthologous Groups (COG), and Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathways. Gene expression profile analysis showed that 18,474 ESTs were differentially expressed, with 10,131 up-regulated in mycelium and 8,343 up-regulated in fruiting body. Putative genes involved in polysaccharide and steroid biosynthesis were identified from A. aegerita transcriptome, and these genes were differentially expressed at the two stages of A. aegerita. Based on one-dimensional gel electrophoresis (1-DGE) coupled with electrospray ionization liquid chromatography tandem MS (LC-ESI-MS/MS), we identified a total of 309 non-redundant proteins. And many metabolic enzymes involved in glycolysis were identified in the protein database.Conclusions/SignificanceThis is the first study on transcriptome and proteome analyses of A. aegerita. The data in this study serve as a resource of A. aegerita transcripts and proteins, and offer clues to the applications of this mushroom in nutrition, pharmacy and industry.

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Evidences of high carbon catabolic enzyme activities during sporulation of Pleurotus ostreatus (Florida)

Cited by 12 publications

References 21 publications

Optimization of biomass production with enhanced glucan and dietary fibres content by Pleurotus ostreatus ATHUM 4438 under submerged culture

Optimization of biomass production with enhanced glucan and dietary fibres content by Pleurotus ostreatus ATHUM 4438 under submerged culture

Potential molecular mechanisms for fruiting body formation of Cordyceps illustrated in the case ofCordyceps sinensis

Transcriptome and Proteome Exploration to Provide a Resource for the Study of Agrocybe aegerita

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