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Abstract:The aim of this study was to investigate the potential of the submerged fermentation procedure in the production of bioactive metabolites of the common edible mushroom Pleurotus ostreatus. The biomass of the mushroom strain was produced by submerged fermentation in a batch stirred tank bioreactor and extracted by solvents of increasing polarity. The dichloromethane and methanol extract were fractioned by different techniques including Adsorption Chromatography and Fast Centrifugal Partition Chromatography (FCPC). The structures of pure compounds were elucidated with 1D/2D NMR-spectroscopic analyses, and chemical correlations combined with GC/MS and LC/MS experiments. Nineteen metabolites (e.g., fatty acids, phenolic metabolites, nucleotides and alkaloids) were isolated. Beyond the production of known metabolites, we report herein the production also of trans -3,4-dihydro-3,4,8-trihydroxynapthalen- indicates the great potential of the established bioprocess for the production of P. ostreatus mycelia with enhanced metabolic profile.

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The synthesis of aliphatic polyesters (PEs) derived from diols (1,4-butanediol and 1,8-octanediol) and diacids or their derivatives (diethyl succinate, sebacic acid, 1,12-dodecanedioic acid, and 1,14-tetradecanedioic acid) was achieved in order to produce poly(butylene succinate) (PE 4.4), poly(octylene sebacate) (PE 8.10), poly(octylene dodecanate) (PE 8.12), and poly(octylene tetradecanate) (PE 8.14). The herein suggested procedure involved two stages, both sustainable and in accordance with the principles of "green" polymerization. The first comprised an enzymatic prepolymerization under vacuum, in the presence of diphenylether as solvent using Candida antarctica lipase B as biocatalyst, whereas a low-temperature postpolymerization step [solid state polymerization (SSP)] followed in order to upgrade the PEs quality. In the enzymatically synthesized prepolymers, the range of number-average molecular weight attained was from 3700 to 8000 g/mol with yields reaching even 97%. Subsequently, SSP of PE 4.4 and 8.12 took place under vacuum or flowing nitrogen and lasted 10-48 h, at temperatures close to the prepolymer melting point (T m 2 T SSP varied between 4 C and 14 C). The solid state finishing led to increase in the molecular weight depending on the prepolymer type, and it also contributed to improvement of the physical characteristics and the thermal properties of the enzymatically synthesized PEs.

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