Evidence of external digestion of crustaceans in <i>Octopus vulgaris</i> paralarvae

Hernández-García, Vicente; Martín, Ana Y.; Castro, J.J.

doi:10.1017/s0025315400002320

Cited by 34 publications

(26 citation statements)

References 7 publications

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“…Paralarvae in captivity have been traditionally fed with enriched Artemia (Iglesias et al, 2006) or supplemented with decapod zoeae, copepods, mysids, shrimps, or fish larvae, which increases survival and growth rates (Hernández-García et al, 2000; Iglesias et al, 2004, 2006; Ikeda et al, 2005; Carrasco et al, 2006; Kurihara et al, 2006; Martínez et al, 2014; Farías et al, 2015). Despite all previous attempts, cephalopod paralarval mortality is still close to 100% in captivity.…”

Section: Introductionmentioning

confidence: 99%

Diet Composition and Variability of Wild Octopus vulgaris and Alloteuthis media (Cephalopoda) Paralarvae: a Metagenomic Approach

et al. 2017

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The high mortality of cephalopod early stages is the main bottleneck to grow them from paralarvae to adults in culture conditions, probably because the inadequacy of the diet that results in malnutrition. Since visual analysis of digestive tract contents of paralarvae provides little evidence of diet composition, the use of molecular tools, particularly next generation sequencing (NGS) platforms, offers an alternative to understand prey preferences and nutrient requirements of wild paralarvae. In this work, we aimed to determine the diet of paralarvae of the loliginid squid Alloteuthis media and to enhance the knowledge of the diet of recently hatched Octopus vulgaris paralarvae collected in different areas and seasons in an upwelling area (NW Spain). DNA from the dissected digestive glands of 32 A. media and 64 O. vulgaris paralarvae was amplified with universal primers for the mitochondrial gene COI, and specific primers targeting the mitochondrial gene 16S gene of arthropods and the mitochondrial gene 16S of Chordata. Following high-throughput DNA sequencing with the MiSeq run (Illumina), up to 4,124,464 reads were obtained and 234,090 reads of prey were successfully identified in 96.87 and 81.25% of octopus and squid paralarvae, respectively. Overall, we identified 122 Molecular Taxonomic Units (MOTUs) belonging to several taxa of decapods, copepods, euphausiids, amphipods, echinoderms, molluscs, and hydroids. Redundancy analysis (RDA) showed seasonal and spatial variability in the diet of O. vulgaris and spatial variability in A. media diet. General Additive Models (GAM) of the most frequently detected prey families of O. vulgaris revealed seasonal variability of the presence of copepods (family Paracalanidae) and ophiuroids (family Euryalidae), spatial variability in presence of crabs (family Pilumnidae) and preference in small individual octopus paralarvae for cladocerans (family Sididae) and ophiuroids. No statistically significant variation in the occurrences of the most frequently identified families was revealed in A. media. Overall, these results provide new clues about dietary preferences of wild cephalopod paralarvae, thus opening up new scenarios for research on trophic ecology and digestive physiology under controlled conditions.

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Section: Introductionmentioning

confidence: 99%

Diet Composition and Variability of Wild Octopus vulgaris and Alloteuthis media (Cephalopoda) Paralarvae: a Metagenomic Approach

et al. 2017

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“…Such teeth have been observed also in paralarvae of other cephalopod species (Boletzky 1974;Young et al 1985;Wakabayashi et al 2002;. Experimental studies on paralarvae of O.vulgaris (Hernandez-Garcia et al 2000) showed that they carry out external digestion and ingest the flesh only. The function of teeth at paralarval stage may be related to an adaptation facilitating the suction of the internal fluids of prey (Boletzky 1974; and removal of semidigested prey meat from exoskeleton as reported in adult Idiosepius paradoxus (Kasugai et al 2004).…”

Section: Discussionmentioning

confidence: 88%

First record of the glass octopus Vitreledonella richardi (Cephalopoda: Vitreledonellidae) from the Arabian Sea

et al. 2016

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“…trituberculatus zoeae and capturing them for ingestion, demonstrating the characteristic feeding behaviour of O. vulgaris paralarvae described by Villanueva, Nozais, and Boletzky (). A large number of zoeae exoskeletons were deposited daily on the filter mesh and tank bottom, the remains of external digestion (Hernández‐García, Martín, & Castro, ; Villanueva, Riba, Ruíz‐Capillas, González, & Baeta, ). These observations suggest that the P .…”

Section: Discussionmentioning

confidence: 99%

Effects of co-supply ratios of swimming crab Portunus trituberculatus zoeae and Artemia on survival and growth of East Asian common octopus Octopus sinensis paralarvae under an upwelling culture system

et al. 2019

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Despite recent advances in culture technology for East Asian common octopus Octopus sinensis paralarvae using upwelling systems, securing suitable feed for the paralarvae is an unresolved issue. The zoea of the swimming crab Portunus trituberculatus is a good candidate for paralarval feed because of the high fecundity of the adult females. To investigate the effects of supplying P. trituberculatus zoeae and their feeding method on paralarvae, we cultured paralarvae with supplying different combination ratios of zoeae and Artemia (10:0, 7:3, 5:5, 3:7 and 0:10), and with or without supplementing rotifers using small‐scale (3‐L) upwelling systems. Paralarval survival rate and growth were improved when zoeae were supplied as the main feed, but reduced when the proportion of Artemia exceeded half the whole preys. Supplementing rotifers did not affect the paralarval survival and growth. Subsequently, paralarvae were cultured by supplying zoeae (partially augmented by Artemia) using three large (1‐kl) upwelling systems to assess their feeding effectiveness in juvenile octopus production. Paralarvae could be cultured at high survival rates of 77.1 ± 5.1% to reach benthic juveniles at 23 days after hatching. In conclusion, supplying P. trituberculatus zoeae augmented with Artemia under an upwelling culture system has great potential for juvenile octopus production.

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Evidence of external digestion of crustaceans in Octopus vulgaris paralarvae

Cited by 34 publications

References 7 publications

Diet Composition and Variability of Wild Octopus vulgaris and Alloteuthis media (Cephalopoda) Paralarvae: a Metagenomic Approach

Diet Composition and Variability of Wild Octopus vulgaris and Alloteuthis media (Cephalopoda) Paralarvae: a Metagenomic Approach

First record of the glass octopus Vitreledonella richardi (Cephalopoda: Vitreledonellidae) from the Arabian Sea

Effects of co-supply ratios of swimming crab Portunus trituberculatus zoeae and Artemia on survival and growth of East Asian common octopus Octopus sinensis paralarvae under an upwelling culture system

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