Evidence for the Critical Role of Sucrose Synthase for Anoxic Tolerance of Maize Roots using a Double Mutant

Ricard, Bérénice; Toai, T. V.; Chourey, Prem S.; Saglio, Pierre H.

doi:10.1104/pp.116.4.1323

Cited by 121 publications

(86 citation statements)

References 36 publications

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“…1B). Suc synthase catalyzes the conversion of Suc to UDP-Glc and Fru that will be used in glycolysis (Ricard et al, 1998). ADH1 and PDC1 are involved in catalyzing reactions involved in ethanol fermentation, which occurs in response to O 2 deprivation to ensure the regeneration of NAD + for glycolysis with concomitant production of ATP, in the absence of mitochondrial respiration (Kü rsteiner et al, Table I.…”

Section: Resultsmentioning

confidence: 99%

Reactive Oxygen Species-Driven Transcription in Arabidopsis under Oxygen Deprivation

et al. 2012

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Reactive oxygen species (ROS) play an important role as triggers of gene expression during biotic and abiotic stresses, among which is low oxygen (O 2 ). Previous studies have shown that ROS regulation under low O 2 is driven by a RHO-like GTPase that allows tight control of hydrogen peroxide (H 2 O 2 ) production. H 2 O 2 is thought to regulate the expression of heat shock proteins, in a mechanism that is common to both O 2 deprivation and to heat stress. In this work, we used publicly available Arabidopsis (Arabidopsis thaliana) microarray datasets related to ROS and O 2 deprivation to define transcriptome convergence pattern. Our results show that although Arabidopsis response to anoxic and hypoxic treatments share a common core of genes related to the anaerobic metabolism, they differ in terms of ROS-related gene response. We propose that H 2 O 2 production under O 2 deprivation is a trait present in a very early phase of anoxia, and that ROS are needed for the regulation of a set of genes belonging to the heat shock protein and ROS-mediated groups. This mechanism, likely not regulated via the N-end rule pathway for O 2 sensing, is probably mediated by a NADPH oxidase and it is involved in plant tolerance to the stress.

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Section: Resultsmentioning

confidence: 99%

Reactive Oxygen Species-Driven Transcription in Arabidopsis under Oxygen Deprivation

et al. 2012

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“…This might indicate that Sus2 is important for O # -shortage stress response in Arabidopsis. A role for Sus during anoxia has clearly been demonstrated in maize roots with the use of single or double Sus mutants, in which anoxic tolerance was correlated with the number of Sus genes [41]. Interestingly, Arabidopsis Sus2 belongs to a small family of Sus genes that diverged very early from the main evolutionary branch for this gene ; only sugar-beet Sus and pea Sus2 belong to the same cluster [26].…”

Section: Role Of Sus Genes In Stress Acclimation In Leavesmentioning

confidence: 99%

Sugar/osmoticum levels modulate differential abscisic acid-independent expression of two stress-responsive sucrose synthase genes in Arabidopsis

Déjardin

Sokolov

Kleczkowski

1999

Biochemical Journal

113

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Sucrose synthase (Sus) is a key enzyme of sucrose metabolism. Two Sus-encoding genes (Sus1 and Sus2) from Arabidopsis thaliana were found to be profoundly and differentially regulated in leaves exposed to environmental stresses (cold stress, drought or O2 deficiency). Transcript levels of Sus1 increased on exposure to cold and drought, whereas Sus2 mRNA was induced specifically by O2 deficiency. Both cold and drought exposures induced the accumulation of soluble sugars and caused a decrease in leaf osmotic potential, whereas O2 deficiency was characterized by a nearly complete depletion in sugars. Feeding abscisic acid (ABA) to detached leaves or subjecting Arabidopsis ABA-deficient mutants to cold stress conditions had no effect on the expression profiles of Sus1 or Sus2, whereas feeding metabolizable sugars (sucrose or glucose) or non-metabolizable osmotica [poly(ethylene glycol), sorbitol or mannitol] mimicked the effects of osmotic stress on Sus1 expression in detached leaves. By using various sucrose/mannitol solutions, we demonstrated that Sus1 was up-regulated by a decrease in leaf osmotic potential rather than an increase in sucrose concentration itself. We suggest that Sus1 expression is regulated via an ABA-independent signal transduction pathway that is related to the perception of a decrease in leaf osmotic potential during stresses. In contrast, the expression of Sus2 was independent of sugar/osmoticum effects, suggesting the involvement of a signal transduction mechanism distinct from that regulating Sus1 expression. The differential stress-responsive regulation of Sus genes in leaves might represent part of a general cellular response to the allocation of carbohydrates during acclimation processes.

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“…Segundo esses autores, a diminuição na atividade da sintase da sacarose pode contribuir no ajustamento das plântulas às condições de baixo oxigênio. Esses resultados confirmam a importância da sintase da sacarose durante a hipoxia de plântulas de milho relatada por RICARD et al (1998). Ao mesmo tempo, GUGLIELMINETTI et al (1995) observaram que a razão de atividade entre a sintase da sacarose e a invertase neutra do citosol, em coleóptilos de arroz de oito dias de idade, foi de 5:1 sob condições aeróbicas e 30:1 sob anoxia.…”

Section: Resultsunclassified

“…Na falta de oxigênio, o metabolismo é desviado para a via anaeróbica que, além de ter baixo rendimento energético, produz substâncias tóxicas, tais como etanol e ácido lático. Em contrapartida, a expressão de genes responsáveis pela síntese de enzimas do metabolismo de carboidratos (ANDREWS et al, 1994;GUGLIELMINETTI et al, 1995;RICARD et al;, assim como a formação de aerênquimas e raízes adventíceas (HE et al, 1994;KOLB et al, 1998;ZENG et al, 1999) parecem estar relacionadas aos mecanismos de tolerância em plantas.…”

Section: Introductionunclassified

“…Todavia, a função dessas enzimas no período de germinação e sua participação no desenvolvimento inicial de plântulas ainda é pouco conhecida. Embora a hipoxia exerça importante influência na regulação da relação fonte-dreno entre o endosperma e a plântula, possivelmente pela modulação da expressão de genes relacionados (MITSUHASHI et al, 2004), as invertases parecem não ter uma atuação significativa nessas condições (GUGLIELMINETTI et al, 1995;RICARD et al, 1998;ZENG et al, 1999). VANTOAI et al (1995) verificaram que a idade da planta pode interferir na tolerância ao estresse anóxico ou hipóxico.…”

Section: Introductionunclassified

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Crescimento de plântulas do milho 'Saracura' e atividade de alfa-amilase e invertases associados ao aumento da tolerância ao alagamento exercido pelo cálcio exógeno

Fries¹,

Alves²,

Filho

et al. 2007

Bragantia

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RESUMOObjetivou-se avaliar o crescimento da plântula e o metabolismo de carboidratos associados ao aumento da tolerância à hipoxia exercido pela presença do cálcio no período de germinação e/ou alagamento de plântulas de milho com diferentes idades. O experimento foi desenvolvido na Universidade Federal de Lavras, MG, em 2002. Cariopses de milho da variedade 'Saracura' (BRS-4154) foram germinadas em água ou solução de CaCl 2 . Após dois e quatro dias, as plântulas foram submetidas ao alagamento em tubos de PVC com tampão (com ou sem CaCl 2 ) por três dias, sendo então avaliadas a sobrevivência, massa seca e as características bioquímicas. O cálcio aumentou a sobrevivência ao alagamento de plântulas com quatro dias, entretanto, não influenciou naquelas com dois dias. O alagamento reduziu a massa seca das plântulas sem prejudicar sua recuperação pós-estresse, entretanto, o cálcio influenciou no acúmulo de massa seca pós-hipoxia somente em plântulas com quatro dias, mostrando uma recuperação mais rápida dos danos causados no período de alagamento, o que ocorre em plântulas de dois dias de germinação, independentemente desse elemento. Em plântulas mais jovens também houve maior mobilização das reservas de amido, assim como, maior translocação e utilização dessas reservas. O alagamento reduziu drasticamente a atividade da invertase ácida do vacúolo, independentemente da presença do cálcio, comprovando sua não participação nos processos comandados por essa enzima. A redução na atividade das invertases favorece menor concentração de hexoses, diminuindo a respiração anaeróbica e, conseqüentemente, a produção de substâncias tóxicas, aumentando a sobrevida das plântulas em condições de hipoxia.Palavras-chave: Invertase, α-amilase, cálcio, hipoxia.( ABSTRACT GROWTH OF MAIZE 'SARACURA' (BRS-4154) AND α-AMYLASE AND INVERTASES ACTIVITY ASSOCIATED WITH THE INCREASE OF FLOODING TOLERANCE CAUSED BY EXOGENOUS CALCIUMThis research evaluated the plantlet growth and carbohydrate metabolism associated with the increase in hypoxia tolerance caused by calcium presence during germination and/or flooding of maize plantlets with different ages. The experiment was conducted at Universidade Federal de Lavras, MG, in 2002. Maize caryopses (var.'Saracura') were germinated in water or CaCl 2 . After two and four days, the plantlets were submitted to flooding in PVC tubes with buffer (with or without CaCl 2 ) during three days, being evaluated the survival, dry weight and biochemical characteristics. Calcium increased plantlet survival to flooding at four days, although there was no influence on two-day samples. Flooding reduced the plantlet dry weight without affect on the recovery after stress, therefore calcium influenced dry weight accumulation after hypoxia only in plantlets at four days, showing a faster recovery from damages caused during the flooding period, which also occurs in plantlets treated at two days of germination, but independently of this element. Younger plantlets also showed higher mobilization of the starch reserves, as w...

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Evidence for the Critical Role of Sucrose Synthase for Anoxic Tolerance of Maize Roots using a Double Mutant

Cited by 121 publications

References 36 publications

Reactive Oxygen Species-Driven Transcription in Arabidopsis under Oxygen Deprivation

Reactive Oxygen Species-Driven Transcription in Arabidopsis under Oxygen Deprivation

Sugar/osmoticum levels modulate differential abscisic acid-independent expression of two stress-responsive sucrose synthase genes in Arabidopsis

Crescimento de plântulas do milho 'Saracura' e atividade de alfa-amilase e invertases associados ao aumento da tolerância ao alagamento exercido pelo cálcio exógeno

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