Evanescent wave fluorescence biosensor combined with DNA bio-barcode assay for platelet genotyping

Bio-barcode detection technology and its research applications: A review

“…Signal amplification detection methods commonly used for the BCA mainly include chip methods [24] , [25] , [26] , [27] , fluorescence labelling methods [28] , [29] , [30] , [31] , colorimetric methods [32] , [33] , [34] , biosensor methods [35] , [36] , [37] , [38] , and immuno-PCR methods [39] , [40] , [41] , [42] . Occasionally, DNA barcodes are amplified by a combination of techniques, such as real-time PCR [43] , [44] , [45] .…”

“… Experimental steps are complicated. [38] Human platelet antigen 2010 Germany 2 × 10 −12 mol/L 150 s [35] The protective antigen A (pagA) gene of Bacillus anthracis and the insertion element (Iel) gene of Salmonella enteritidis 2010 USA 0.5 ng/mL; 50 pg/mL 1 h [36] Escherichia coli O157:H7 2018 China 50 CFU/mL 1 h [37] IPCR Hantaan virus nucleocapsid protein HCV core antigen 2009 China 10 fg/mL 1.5 h Detection of target by antigen-antibody specificity and PCR amplification technology. Sometimes used in conjunction with fluorescence PCR technology.…”

“…In recent years, there has been continuous development and exploration in the fields of medicine, clinical detection, molecular biology, immunology, and nanotechnology, among others [1] , [2] , [3] , [4] , [5] , [6] . There are now higher requirements for the trace analysis of macromolecules and small molecules, such as proteins and nucleic acids, as well as agricultural and veterinary drugs and environmental pollutants.…”

A Spellbinding Interplay Between Biological Barcoding and Nanotechnology

“…Researchers have developed a very highly sensitive and selective biosensor for the detection of microRNAs (miRNAs) utilizing bio-barcode DNA assay with catalytic hairpin assembly via multiple probes (Dong et al, 2015). A new development of bio-barcoded biosensors for the detection of DNA in poor biological samples has been proclaimed using nanomagnetic beads (Trévisan et al, 2010).…”

Smartphone fluorescence spectroscopy

“…Light-emitting reporters are the most commonly used tools for studying the interactions between biomolecules in applications that include genomics, 3 proteomics, 4 and drug discovery. 5 Light emitters operate via a variety of optical mechanisms that include fluorescence, 6 chemoluminescence, 7 and semiconductor quantum dot 8 excited electron relaxation. Among these approaches, those that utilize the Förster Resonance Energy Transfer (FRET) as a mechanism for observing changes in the quenching efficiency between matched donor-acceptor pairs are effective methods for diagnostics applications because single-step assays are performed in liquid, without complex mixing-washing steps.…”