Evaluation of Urine Cytology in Urothelial Carcinoma Patients: A Comparison of CellprepPlus® Liquid-Based Cytology and Conventional Smear

Abstract

BackgroundUrine cytology is an important test in the screening of urothlelial neoplasms. The conventional smear (CS) method of testing urine samples has a low sensitivity, approximately 50% result accuracy for detecting urothelial carcinomas, while liquid-based cytology (LBC) has much improved diagnostic accuracy, sensitivity, and specificity. The aim of this study was to compare the morphologic features and diagnostic efficacy of CellprepPlus® LBC with those of CS for urine cytology.MethodsA total of 713 cases of urine specimens collected from November 2009 to September 2010 were included. All specimens were divided equally for the preparation of CellprepPlus® LBC and CS for each case.ResultsCellprepPlus® revealed more cellularity, a cleaner background and better cytomorphologic features, but it showed a less intact architectural pattern compared to that of CS. Of the 88 histologically confirmed cases, the diagnostic sensitivity for CellprepPlus® was 50% and higher than the 37.5% for CS. The specificity of both preparations was 100%.ConclusionsThe CellprepPlus® showed an improved quality of slides and provided better diagnostic accuracy, thus CellprepPlus® could be a first-line screening tool in urinary tract cytology.

Comparison of diagnostic accuracy between CellprepPlus® and ThinPrep® liquid‐based preparations in effusion cytology

“…Consistent with previous studies, we found that CellprepPlus® LBC yielded larger tumor cell nuclei in 20 of the 30 cases and more hypochromatic tumor cell nuclei in 18 of the 30 cases compared to ThinPrep® LBC. Therefore, the nucleoli and nuclear membrane of the tumor cells were more prominent in CellprepPlus® LBC preparations compared to ThinPrep® LBC preparations in 20 of the 30 cases.…”

“…Koo et al reported that preparation of body fluids using CellprepPlus® LBC yielded better sensitivity and negative predictive values, as well as a higher slide quality, than the conventional smear. The use of CellprepPlus® LBC in preparing urine cytology specimens also resulted in improved slide quality and diagnostic accuracy compared to the conventional smear . Additionally, CellPrep® LBC produced results comparable to those of ThinPrep® LBC in terms of smear quality and diagnostic efficacy in cervicovaginal smear, body fluid, and urine specimen preparations…”

“…Many studies have compared liquid‐based preparation methods, such as ThinPrep® LBC, and the conventional smear, and some reports have also examined CellprepPlus® LBC . However, to our knowledge, only one study has compared the accuracy and unsatisfactory rates in cytology between CellPrep® LBC and ThinPrep® LBC .…”

“…Many studies have compared liquid-based preparation methods, such as ThinPrepV R LBC, and the conventional smear, [3][4][5][6][7][8][9][10][11] and some reports have also examined CellprepPlusV R LBC. 12,13 However, to our knowledge, only one study has compared the accuracy and unsatisfactory rates in cytology between CellPrepV R LBC and ThinPrepV R LBC. 14 Therefore, this study compared the sensitivity and specificity of the CellprepPlusV R and ThinPrepV R LBC methods in effusion cytology and investigated the differences in cytomorphological features between samples prepared using both methods.…”

Reliability of Estrogen Receptor and Human Epidermal Growth Factor Receptor 2 Expression on Breast Cancer Cells Stored in Cellprep® Vials

“…Once the CP vials were placed into the CellprepPlus® device, the cells they contained were stirred by an air jet from the outside. After collecting the cells on a filter attached to the outside of the CP vial with absorbing wind pressure, they were blown from the filter to the slide using air pressure and transferred to a circular area of 20 mm in a thin layer [18]. Each CP slide was quickly postfixed with 95% ethanol for over 30 min.…”