Romanowsky staining using liquid‐based cytology: A pilot study using Cytolyt®/HESPANDER® processing solution for ThinPrep® preparations

Kinoshita, Yuichi; Yuri, Takashi; Yoshizawa, Katsuhiko; Takasu, Kosho; Emoto, Yuko; Tsubura, Airo; Shikata, Nobuaki

doi:10.1002/dc.23368

Cited by 4 publications

(5 citation statements)

References 30 publications

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“…Advantages of the LBC‐RTF procedure are that simple workflow and stable staining results. LBC‐RTF is not a substitute for frozen sections or for other special stains that may be used on cytology samples during intraoperative consultation (eg, May Grünwald Giemsa or Diff Quik stain), but is a useful and convenient ancillary tool available to pathologists to aid in intraoperative consultation. This study is the first such investigation, except for a previous study on sentinel lymph nodes.…”

Section: Discussionmentioning

confidence: 99%

Intraoperative liquid‐based cytology of rinsed tissue fragments from head and neck lesions

Kinoshita

Inaba

Kobayashi

et al. 2018

Diagnostic Cytopathology

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Background Intraoperative diagnosis is an essential tool for the rapid diagnostic assessment of clinically critical head and neck lesions. Thus, we assumed that a combination of frozen section histological and cytological diagnoses may be preferable. Here, we investigated a relatively new method called liquid‐based cytology of rinsed tissue fragments (LBC‐RTF) and compared this method with intraoperative histological diagnosis. Methods We used 68 tissue biopsies (9 brains, 8 lymph nodes, 22 salivary glands, and 29 thyroid samples). Samples submitted for intraoperative consultation were divided into two‐halves: one was used to prepare frozen sections, and the other was prepared for LBC‐RTF by washing with PreservCyt. We then compared the final diagnosis obtained from permanent sections with the intraoperative histological diagnosis based on frozen sections and examination of LBC‐RTF preparations. Results The accuracy of LBC‐RTF was higher than that of intraoperative histological diagnosis alone, based on frozen sections of every organ (LBC‐RTF: 91.2% vs intraoperative histological diagnosis: 80.9%). With LBC‐RTF, artifacts that are commonly observed in frozen sections were not present. In addition, even with challenging cases from which it is impossible to prepare frozen sections, intraoperative diagnosis was possible using the LBC‐RTF technique. Conclusion Both histological and cytological intraoperative diagnoses were possible during a surgery if the LBC‐RTF technique was used. Moreover, our findings suggest that LBC‐RTF improved the diagnostic accuracy of traditional intraoperative diagnosis.

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Section: Discussionmentioning

confidence: 99%

Intraoperative liquid‐based cytology of rinsed tissue fragments from head and neck lesions

Kinoshita

Inaba

Kobayashi

et al. 2018

Diagnostic Cytopathology

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“…[4][5][6] Haemolysis reagents such as Cytolyt are traditionally used for cytological specimens such as those processed via liquid-based methodologies. 16 However, EUS-FNAB specimens are increasingly taken through wider core needles and therefore processed directly as cell blocks without a haemolysis step. [4][5][6]9 Indeed, our longstanding experience of assessing EUS-FNAB specimens indicates that the extra haemolysis step compromises morphological integrity of cells/tissue in the resulting slides and may also decrease their final cellularity; 5 for the same reason as above, this is a particular issue for SCA specimens.…”

Section: Discussionmentioning

confidence: 99%

“…Secondly, for EUS‐FNAB specimens prepared without lysis of erythrocytes, GLUT1 expression by these cells greatly hampers assessment for neoplastic cells; this is particularly an issue for SCA specimens, which typically only contain scattered small clusters of neoplastic cells 4–6 . Haemolysis reagents such as Cytolyt are traditionally used for cytological specimens such as those processed via liquid‐based methodologies 16 . However, EUS‐FNAB specimens are increasingly taken through wider core needles and therefore processed directly as cell blocks without a haemolysis step 4–6,9 .…”

Section: Discussionmentioning

confidence: 99%

Calponin and MUC6 complement inhibin as diagnostic immunomarkers of serous cystadenoma in endoscopic ultrasound‐guided aspiration/biopsy specimens

et al. 2021

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Calponin and MUC6 complement inhibin as diagnostic immunomarkers of serous cystadenoma in endoscopic ultrasound-guided aspiration/biopsy specimens Aims: Because serous cystadenoma (SCA) does not usually require excision, it is critical to distinguish it from differential diagnoses which do, especially neuroendocrine tumour (NET). The gold standard for diagnosing SCA is assessment of endoscopic ultrasound-guided fine needle aspiration/biopsy (EUS-FNAB) material. Inhibin immunohistochemistry aids this assessment, but such positivity is not absolutely sensitive or specific to SCA. The following is the largest known study of SCA EUS-FNAB specimens and the first to compare four potential SCA immunomarkers between themselves and inhibin, compared against NET. Methods and results: Immunohistochemistry for calponin, mucin 6 (MUC6), glucose transporter 1 (GLUT1) and vascular endothelial growth factor A (VEGFA) was performed on 30 EUS-FNAB and three resection specimens of SCA and 32 EUS-FNAB specimens of NET. GLUT1 and VEGFA were suboptimal as diagnostic immunomarkers of SCA, being expressed by 10 and 44% of NETs, respectively. Further, their expression by cellular constituents of blood which often contaminate EUS-FNAB specimens hampered identification of neoplastic cells, especially in hypocellular samples. While 19% of NETs showed nuclear MUC6 positivity, cytoplasmic expression of the protein showed 100% specificity and sensitivity as an SCA marker. However, assessing MUC6 in EUS-FNAB specimens must also consider the protein's focal expression in physiological pancreatic, gastric or duodenal tissues, which can contaminate these specimens. Calponin was less sensitive (71% versus 100%) but more specific (100% versus 91%) than inhibin, although easier to assess in EUS-FNAB specimens than MUC6. Conclusions: Of the four potential immunomarkers of SCA suggested by the existing literature, calponin and MUC6 are useful complementary studies to inhibin for application to EUS-FNAB specimens.

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“…LBC, previously used for cervical cancer screening, has been used as a noninvasive method to detect urothelial lesions . The overall sensitivity of LBC in the detection of UTUCs varies from 36% to 64% .…”

Section: Discussionmentioning

confidence: 99%

“…LBC, previously used for cervical cancer screening, has been used as a noninvasive method to detect urothelial lesions. [22][23][24] The overall sensitivity of LBC in the detection of UTUCs varies from 36% to 64%. 9,[25][26][27] Minimal or moderate cellular atypia in LGUTUCs usually overlaps with reactive urothelial changes, leading to the great interobserver and intra-observer variation in sensitivity.…”

Section: Discussionmentioning

confidence: 99%

The combined application of urinary liquid‐based cytology with fluorescence in situ hybridization and p16/Ki‐67 dual immunostaining is valuable for improving the early diagnosis of upper tract urothelial carcinomas

Sun

Liu

Xia

et al. 2017

Diagnostic Cytopathology

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Romanowsky staining using liquid‐based cytology: A pilot study using Cytolyt^®/HESPANDER^® processing solution for ThinPrep^® preparations

Abstract: Romanowsky staining is necessary for the cytodiagnosis of some conditions. MGG-LBC may contribute to the cytodiagnostic results using LBC preparations.

Cited by 4 publications

References 30 publications

Intraoperative liquid‐based cytology of rinsed tissue fragments from head and neck lesions

Intraoperative liquid‐based cytology of rinsed tissue fragments from head and neck lesions

Calponin and MUC6 complement inhibin as diagnostic immunomarkers of serous cystadenoma in endoscopic ultrasound‐guided aspiration/biopsy specimens

The combined application of urinary liquid‐based cytology with fluorescence in situ hybridization and p16/Ki‐67 dual immunostaining is valuable for improving the early diagnosis of upper tract urothelial carcinomas

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