Reliability of Estrogen Receptor and Human Epidermal Growth Factor Receptor 2 Expression on Breast Cancer Cells Stored in Cellprep® Vials

Abstract

Objective: The aim of this study was to evaluate the reliability of estrogen receptor (ER) and human epidermal growth factor receptor 2 (HER2) expression levels in Cellprep® (CP). Study Design: We evaluated the stability of immunocytochemistry (ICC) of ER and HER2 for primary or recurrent breast cancer samples rinsed in CP vials. Samples were prepared from CP vials stored for 1–30 or 160–240 days. ER and HER2 statuses were determined after 1–30 days (26 and 25 tests, respectively) or 160–240 days (15 and 18 tests, respectively) with the same protocols as immunohistochemistry (IHC), and were compared with the corresponding surgically resected specimens. Results: ER statuses according to CP samples showed perfect agreement (1–30 days: kappa, κ = 1; 160–240 days: κ = 1). HER2 statuses also showed good agreement (1–30 days: κ = 0.79; 160–240 days: κ = 0.64), although there were more equivocal HER2 cases in CP than in the surgically resected specimens. Conclusion: ER ICC in CP vials is reliable regardless of the preservation period. HER2 ICC in CP has more equivocal cases than HER2 IHC in surgically resected specimens. Both improvement of the immunostaining protocol and further validation study of in situ hybridization are indispensable for the practical application of ICC in CP.

Immunoreactivity of TTF‐1, GATA‐3, CEA, and p16/Ki67 cocktail in Cellprep®‐processed control samples: Comparison of long‐term storage in vials and slides

“…To date, there have been several reports on the storage conditions of LBC samples for the ICC quality control, analyzed using cell lines [14][15][16][17] or clinical samples. 9,18,19 However, these studies used LBC samples processed with ThinPrep ®14-16,18 or SurePath™. 17,19 We previously used LBC samples prepared using CP to examine the storage period of samples for the ICC in detecting estrogen receptor (ER) and human epidermal growth factor receptor 2 (HER2).…”

“…17,19 We previously used LBC samples prepared using CP to examine the storage period of samples for the ICC in detecting estrogen receptor (ER) and human epidermal growth factor receptor 2 (HER2). 9 There are no publications specific to ICC controls in LBC samples using CP. This study is the first to investigate in detail storage conditions suitable for the ICC in LBC samples prepared with CP.…”

“…According to previous reports, LBC samples were stored either in a preservation solution or on a slide. Moreover, the storage temperature was set to RT for samples stored in preservation solution, 9,14,16,17 and various temperatures for samples stored on slides. 14,15,18,19 Zappacosta et al 16 investigated whether Thinprep ® -processed samples stored in PreservCyt solution at RT could detect antigens after a long storage period.…”

“…The cells collected in the CP vials are agitated using wind pressure, collected onto an externally mounted filter with negative pressure, and smeared onto a slide glass. 9 As a result, cells are not subject to excessive strain. In this study, the immunoreactivity of clinical samples stored long-term for ICC controls using CP was investigated with a focus on two storage conditions: CP vial storage at room temperature (RT: 20-25 C) and CP slide storage in the refrigerator (2-6 C).…”

Breast metastasis from rectal carcinoma: A case report and review of the literature

“…The first step in diagnosis is to definitively identify whether the breast mass is a primary tumor or metastasis; this can be accomplished by combining the results of pathology and IHC. ER, PR and Her-2 are biomarkers for breast cancer, and CK20, CDH17, SATB2 and CDX-2 are biomarkers for rectal cancer[ 8 , 9 ]. Additionally, some other suspected immunohistochemical makers depended on what cancer is suspected.…”