Evaluation of Three Rapid Methods for the Direct Identification of
            <i>Staphylococcus aureus</i>
            from Positive Blood Cultures

Chapin, Kimberle; Musgnug, Michael C.

doi:10.1128/jcm.41.9.4324-4327.2003

Cited by 87 publications

(67 citation statements)

References 19 publications

(10 reference statements)

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“…The sensitivity of this agglutination method after a short period of subculture incubation is higher than that reported for direct testing by Staphaurex Plus on the bacterial pellet (23%) (5) and is also higher than that reported for the tube coagulase test (65 to 85%) (1,4) and comparable to the sensitivities of fluorescent in situ hybridization (98%) (1,3) and the thermostable DNase test (100%) (2). Although this technique utilized the Slidex Staph Plus test, which is considered a reliable agglutination test (6), other agglutination tests will probably have comparable results.…”

Section: Rapid Identification Of Staphyloccocus Aureus In Positive-tementioning

confidence: 52%

Rapid Identification of Staphyloccocus aureus in Positive-Testing Blood Cultures by Slidex Staph Plus Agglutination Test

et al. 2008

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In a prospective study from April 2003 until February 2004, we investigated the accuracy of the Slidex Staph Plus agglutination test (bioMérieux, Marcy-l'Étoile, France) on positive blood cultures containing gram-positive cocci in clusters resembling staphylococci. This agglutination test utilizes latex particles sensitized with human fibrinogen and S. aureus-specific monoclonal antibodies. These monoclonal antibodies detect protein A by the Fc fragment of immunoglobulin G as well as different polysaccharide antigens on the bacterial cell surface. Parallel to a routine subculture on blood agar plates (Columbia III blood agar base with 5% sheep red blood cells; Becton Dickinson), 6 ml from each positive Bactec (Becton Dickinson, Sparks, MD) bottle was injected in a serum separator tube (Becton Dickinson). This tube was centrifuged at 2,000 ϫ g for 10 min. Subsequently, the supernatant was removed and a sample from the top of the separator layer containing the bacteria was taken with a sterile swab and subcultured on a blood agar plate. After incubation for a minimum of 4 h and a maximum of 6 h at 35°C with 5% CO 2 , growth from this blood agar plate was tested with the Slidex Staph Plus test. The following day, the same agglutination test was performed on colonies from blood agar plates routinely subcultured and incubated overnight. All agglutination-positive isolates were confirmed to be S. aureus with a probe hybridization assay (AccuProbe; Gen-Probe, San Diego, CA).A total of 249 positive blood cultures containing staphylococci were evaluated. Growth from a 4-to 6-h subculture on blood agar was compared with growth from an overnight subculture. Four blood cultures yielding coagulase-negative staphylococci were excluded from the analysis due to insufficient growth in the direct assay. There were 56 agglutination-testpositive overnight cultures, of which one was negative by the probe hybridization assay. Two of these agglutination-test-positive overnight cultures were negative by the 4-to 6-h test. There were 189 overnight cultures negative by the agglutination test. One blood culture was Slidex positive by the 4-to 6-h test, but after overnight culture, it showed autoagglutination. The culture was, erroneously, not tested for autoagglutination, and the isolate was probe hybridization negative. The sensitivity, specificity, and positive and negative predictive values for the agglutination test culture with a short incubation period (4 to 6 h) compared with the test agglutination test with overnight culture as the reference method were 96% (95% confidence interval [CI], 87 to 99%), 99% (95% CI, 97 to 99.9%), 98% (95% CI, 89 to 99.9%), and 99% (95% CI, 96 to 99.8%), respectively.The direct Slidex Staph Plus test method is easy to perform and provides reliable same-day identification results in comparison with the reference method. The sensitivity of this agglutination method after a short period of subculture incubation is higher than that reported for direct testing by Staphaurex Plus on the bacterial pellet (23%...

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Section: Rapid Identification Of Staphyloccocus Aureus In Positive-tementioning

confidence: 52%

Rapid Identification of Staphyloccocus aureus in Positive-Testing Blood Cultures by Slidex Staph Plus Agglutination Test

et al. 2008

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“…However, a lower sensitivity (84.1%) for DTC test using undiluted blood culture broth was reported by Chapin and Musgnug [3,7] which may be due to carryover of the inhibitors of coagulation used in blood culture media.…”

Section: Discussionmentioning

confidence: 97%

“…Identification methods should be economical and yield quick results, especially in a developing country like India. DTC is a tube coagulase tests done using the blood culture broth directly, reducing the turnaround time (TAT) by 24 hours [3]. It is simple and an inexpensive test.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of Direct Tube Coagulase Test in Diagnosing Microbiology Section Staphylococcal Bacteremia

Sandeep¹

2014

JCDR

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“…Although many major advancements in technology have made rapid microbiological and molecular testing available for use in the hospital setting, PNA FISH is the only technique with published data describing its clinical impact in rapidly identifying BSIs [16][17][18][19].…”

Section: Impact Of Rapid Microbiological and Molecular Techniquesmentioning

confidence: 99%

The Utility of Rapid Microbiological and Molecular Techniques in Optimizing Antimicrobial Therapy

Eiland

Beyda

Han³

et al. 2010

SRX Pharmacology

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Early treatment of bloodstream infections with appropriate, definitive antimicrobial therapy has proven to reduce mortality, length of hospital stay, and healthcare costs. Culture-based testing methods require up to five days for final pathogen identification and susceptibility reporting, forcing use of broad spectrum empiric therapy. Recently, multiple rapid microbiological and molecular testing methods have been developed that reduce the time to identify the pathogen and susceptibility, allowing optimal antimicrobial therapy to be prescribed earlier. Real-time polymerase chain reaction and gene microarray have been described in literature, yet only peptide nucleic acid fluorescent in-situ hybridization has published data justifying its use based on clinical outcomes and cost savings. Target enriched multiplex polymerase chain reaction was developed to identify both the pathogen and multiple genes associated with resistance from blood within 6 hours and this methodology was studied in our hospital to assess effectiveness at optimizing antimicrobials in staphylococcal bloodstream infections.

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Evaluation of Three Rapid Methods for the Direct Identification of Staphylococcus aureus from Positive Blood Cultures

Cited by 87 publications

References 19 publications

Rapid Identification of Staphyloccocus aureus in Positive-Testing Blood Cultures by Slidex Staph Plus Agglutination Test

Rapid Identification of Staphyloccocus aureus in Positive-Testing Blood Cultures by Slidex Staph Plus Agglutination Test

Evaluation of Direct Tube Coagulase Test in Diagnosing Microbiology Section Staphylococcal Bacteremia

The Utility of Rapid Microbiological and Molecular Techniques in Optimizing Antimicrobial Therapy

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