Evaluation of three methods for preservation of Azotobacter: freeze-drying, cryopreservation, and immobilization in dry polymers

Rojas‐Tapias, Daniel F.; Ortiz-Vera, Mabel; Rivera, Diego; Kloepper, Joseph W.; Bonilla, Ruth

doi:10.11144/javeriana.sc18-2.etmp

Cited by 8 publications

(5 citation statements)

References 36 publications

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“…0025, agar 15) for 48 h at 30 ºC. The concentrations of carrageenin and alginate were selected according to Rojas-Tapias et al (2013), and the concentration of HPMC was based on previous experiments in our lab (data not shown). Sodium alginate and carrageenin were purchased from FMC BioPolymers (Ewing, USA), and HPMC from Sigma-Aldrich (St. Louis, USA).…”

Section: Methodsmentioning

confidence: 99%

“…We previously studied the preservation of A. chroococcum C26 in dry polymers, and observed that alginate and carrageenin are suitable polymers to maintain the viability and activity of A. chroococcum C26 at room temperature (Rojas-Tapias et al 2013). In this study, our objective was to perform a thermodynamic analysis to determine the efficiency of alginate, carrageenin, and a new polymer (HPMC) to maintain the viability of C26, select the best polymer in basis on the Arrhenius equation and its activation energy, and predict the viability of C26 cells in each polymer throughout the time using the Arrhenius plot.…”

Section: Preservation Of Azotobacter Chroococcum Vegetative Cells In mentioning

confidence: 99%

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Preservation of Azotobacter chroococcum vegetative cells in dry polymers

et al. 2014

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We studied the preservation of Azotobacter chroococcum C26 using three dry polymers: carrageenin, sodium alginate, and HPMC, using a method of accelerated degradation. Bacterial viability, as response variable, was measured at three temperatures in four different times, which was followed by calculation of bacterial degradation rates. Results showed that temperature, time of storage, and protective agent influenced both viability and degradation rates (P;lt;0.05). We observed, using the Arrhenius thermodynamic model, that the use of polymers increased the activation energy of bacterial degradation compared to control. We obtained thermodynamic models for each polymer, based on the Arrhenius equation, which predicted the required time for thermal degradation of the cells at different temperatures. Analysis of the models showed that carrageenin was the best polymer to preserve A. chroococcum C26 since ~ 900 days are required at 4 ºC to reduce its viability in two log units. We conclude, therefore, that long-term preservation of A. chroococcum C26 using dry polymers is suitable under adequate preservation and storage conditions.

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Section: Methodsmentioning

confidence: 99%

Section: Preservation Of Azotobacter Chroococcum Vegetative Cells In mentioning

confidence: 99%

Preservation of Azotobacter chroococcum vegetative cells in dry polymers

et al. 2014

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“…and Azotobacter sp. Azotobacter has significant roles in availability of some nutritions like Nitrogen, Phosphorus, Sulphur and carbon via boosting mineralization of organic residues (Fekete et al, 1989;Levaiet al, 2008;Rojas-Tapias et al, 2013).Azotobacter genus belongs to the γ-subclass of the Proeobacteria (Tchan and New, 1984;Becking, 2004) which constitutes seven species namely, A. chroococcum, A. vinelandii, A. beijerinckii, A. paspali, A. armeniacus, A. nigricans, and A. salinestri (Jimenez et al, 2011). A. chroococcum is the most inhabitingvarious soils (Balandreau, 1986;Tchan and New, 1984;Dobereiner, 1995;Martyniuk and Martyniuk, 2003).…”

Section: Azotobactermentioning

confidence: 99%

Archaea, bacteria and termite, nitrogen fixation and sustainable plants production

Sun

Shahrajabian

Cheng

2021

Not Bot Horti Agrobo

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Certain bacteria and archaea are responsible for biological nitrogen fixation. Metabolic pathways usually are common between archaea and bacteria. Diazotrophs are categorized into two main groups namely: root-nodule bacteria and plant growth-promoting rhizobacteria. Diazotrophs include free living bacteria, such as Azospirillum, Cupriavidus, and some sulfate reducing bacteria, and symbiotic diazotrophs such Rhizobium and Frankia. Three types of nitrogenase are iron and molybdenum (Fe/Mo), iron and vanadium (Fe/V) or iron only (Fe). The Mo-nitrogenase have a higher specific activity which is expressed better when Molybdenum is available. The best hosts for Rhizobium legumiosarum are Pisum, Vicia, Lathyrus and Lens; Trifolium for Rhizobium trifolii; Phaseolus vulgaris, Prunus angustifolia for Rhizobium phaseoli; Medicago, Melilotus and Trigonella for Rhizobium meliloti; Lupinus and Ornithopus for Lupini, and Glycine max for Rhizobium japonicum. Termites have significant key role in soil ecology, transporting and mixing soil. Termite gut microbes supply the enzymes required to degrade plant polymers, synthesize amino acids, recycle nitrogenous waste and fix atmospheric nitrogen. The positive effects of Arbuscular mycorrhizal (AM) fungi such as growth promotion, increased root length, leaf area, stem diameter, transplant performance and tolerance to stresses have been reported previously.

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“…In the case of non-leguminous plants, there is no symbiotic relation, therefore the plant is inoculate with free-living N2 fixing bacteria, such as Azotobacter and Azospirillium. These were inoculated in patula pine (Orozco & Martínez, 2009), pastures (Garrido et al 2010, Cárdenas et al 2010, Cárdenas et al 2014), stevia (Borda et al 2011), vegetables (Jiménez et al 2011, eucalyptus (Obando et al 2010, Rojas et al 2013, and maize (Rojas et al 2012). In high efficiency plants having photosynthesis (C4 plants) sugar cane and corn, successful results have been obtained when inoculated into the rhizosphere.…”

Section: Analysis Of Co-occurrencementioning

confidence: 99%

Scientometric analysis of Colombian research on bio-inoculants for agricultural production

et al. 2016

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The excessive use of synthetic chemical inputs in agricultural production has led to the disruption of biogeochemical cycles. One of the alternatives that arose within the systems of sustainable agriculture was the partial or total replacement of chemicals by biological substances. The analysis of relevant scientific literature has become a tool for assessing the quality of knowledge generation and its impact on the environment. A scientometric analysis was conducted of Colombian research on bio-inoculants from 2009 through 2014 in journals added to the Web of SciencesTM in order to identify the characteristics of the main target crops, the microorganisms used, and the beneficial effects on agriculture. In this work, 34 articles were identified: 24 (71%) were research on bio-fertilizer development and 10 (29%) on bio-pesticides. Articles mainly focused on the study of Gram-negative bacilli affecting the area (77%), while others focused on issues and topics surrounding vegetables (30%).The analysis of co-occurrence of keywords identified: i) several genera of microorganisms (e.g. Azotobacter Bradyrhizobium) and sustainable agriculture as issues that have a leading role in this scientific field, ii) plant growth promoting rhizobacteria (PGPR) as an emerging issue, iii) biological nitrogen fixation (BNF) as a subject which has risen in a complementary manner and iv) endophytic bacteria and biodiversity as issues in growth. This study showed that research in Colombia could be targeted on issues such as endophytic bacteria, diversity and productivity.

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Evaluation of three methods for preservation of Azotobacter: freeze-drying, cryopreservation, and immobilization in dry polymers

Cited by 8 publications

References 36 publications

Preservation of Azotobacter chroococcum vegetative cells in dry polymers

Preservation of Azotobacter chroococcum vegetative cells in dry polymers

Archaea, bacteria and termite, nitrogen fixation and sustainable plants production

Scientometric analysis of Colombian research on bio-inoculants for agricultural production

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