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Abstract:Chile contains some of the driest areas in the world, yet human activities in these areas require large volumes of water, the result is regions experiencing high water scarcity leading to environmental degradation, conflicts and reduced industrial productivity. The aim of this paper was to quantify the water scarcity in the central and northern regions by calculating the water scarcity index-the ratio of annual water demand to availability. A focus of the paper was to determine the impact of the main industries in each region and investigate the benefit of implementing water reduction strategies within these industries. The water resources of each investigated region were found to be greatly overexploited and particularly so in the region of Antofagasta. The mining industry was found to be the greatest water consuming sector in this region and further analysis demonstrated that the degree of water scarcity could be greatly reduced by the implementation of water reduction strategies. The agricultural sector dominated water demand in all other regions and it was found that upgrading irrigation efficiency alongside reducing consumption in mining improved the situation in all regions. Nevertheless, given the scale of water scarcity, further investigation is necessary to obtain more recent and accurate data and analyze alternative strategies.

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Indole-3-acetic acid (IAA) is one of the most important molecules produced by Azospirillum sp., given that it affects plant growth and development. Azospirillum brasilense strains Sp245 and Az39 (pFAJ64) were pre-incubated in MMAB medium plus 100 mg/mL L-tryptophan and treated with or exposed to the following (a) abiotic and (b) biotic stress effectors: (a) 100 mM NaCl or NaSO 4.0% (w/v) PEG 0.5 mM HO 0.1 mM abscisic acid, 0.1 mM 1-aminocyclopropane 1-carboxylic acid, 45 °C or daylight, and (b) 4.0% (v/v) filtered supernatant of Pseudomonas savastanoi (Ps) or Fusarium oxysporum (Fo), 0.1 mM salicylic acid (SA), 0.1 mM methyl jasmonic acid (MeJA), and 0.01% (w/v) chitosan (CH). After 30 and 120 min of incubation, biomass production, cell viability, IAA concentration (µg/mL), and ipdC gene expression were measured. Our results show that IAA production increases with daylight or in the presence of PEG, ABA, SA, CH, and Fo. On the contrary, exposure to 45 °C or treatment with HO NaCl, NaSO ACC, MeJA, and Ps decrease IAA biosynthesis. In this report, growth and IAA biosynthesis in A. brasilense under biotic and abiotic stress conditions are discussed from the point of view of their role in bacterial lifestyle and their potential application as bioproducts.

Different sets of parameters and conceptualizations of a basin can give equally good results in terms of predefined objective functions. Therefore, a need exists to tackle equifinality and quantify the uncertainty bands of a model. In this paper we use the concepts of equifinality, identifiability and uncertainty to propose a simple method aimed at constraining the equifinal parameters and reducing the uncertainty bands of model outputs, and obtaining physically possible and reasonable models. Additionally, the uncertainty of equifinal solutions is quantified to estimate the amount by which output uncertainty can be reduced by knowing how to discard most of the equifinal solutions of a model. As a study case, a conceptual model of the Chillán basin in Chile is carried out. From the study it is concluded that using identifiability analysis makes it possible to constrain equifinal solutions with reduced uncertainty and realistic models, resulting in a framework that can be recommended to practitioners, especially due to the simplicity of the method. En outre, l'incertitude de solutions équivalentes est quantifiée pour estimer dans quelle mesure l'incertitude de sortie peut être réduite en sachant comment rejeter la plupart des solutions équivalentes d'un modèle. Comme étude de cas, nous avons construit un modèle conceptuel du bassin du Chillán au Chili. De cette étude, nous concluons que l'utilisation de l'analyse d'identifiabilité permet de contraindre les solutions équivalentes avec une incertitude réduite et des modèles réalistes, ce qui définit un cadre pouvant être recommandé aux praticiens, en particulier en raison de la simplicité de la méthode.

We present the complete genome sequence of Azospirillum brasilense Az39, isolated from wheat roots in the central region of Argentina and used as inoculant in extensive and intensive agriculture during the last four decades. The genome consists of 7.39 Mb, distributed in six replicons: one chromosome, three chromids, and two plasmids.

This article reviews the state of the art with respect to the environmental effects of irrigated agriculture on water and soil quality in arid and semi-arid regions on a field scale. Information is scarce and fragmentary. Examples in selected areas of other arid and semi-arid regions in the world clearly show the importance of studying the environmental impact of irrigation practices on water and soil quality. Studies mainly refer to waterlogging and salinization. As regards agrochemicals, fertilizers have been taken into account through nitrate leaching. The impact of micropollutants such as pesticides and heavy metals on water and soil quality is studied mainly by modeling. Pharmaceutical compounds emerge as pollutants when wastewater is used for irrigation without any previous treatment, situation which is now the object of new studies. There is an obvious need to study in-depth knowledge related to appropriate technologies for the use, treatment, and reuse of wastewater which is a valuable resource in arid and semi-arid regions.