Evaluation of the Sysmex XT-2000iV haematology analyser for rat, dog and mouse whole blood samples

Mathers, R. A.; Evans, G. A.; Bleby, John; Tornow, T.

doi:10.1007/s00580-008-0734-2

Cited by 24 publications

(58 citation statements)

References 6 publications

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“…As in previous studies, RBC, HGB, and MCH were quite stable in canine blood stored at RT . Changes due to erythrocyte swelling during storage were similar to those previously reported for HCT, MCV, and MCHC.…”

Section: Discussionsupporting

confidence: 89%

“…The stability of RET has been little studied in veterinary clinical pathology. The RET percentages measured in healthy dogs with the XT‐2000iV were mildly to markedly increased at 4°C or 22°C at T24 and T48 . In people, RET were shown to be stable at 4°C up to 72 hours and unstable at RT, with decreasing counts from T24 on.…”

Section: Discussionmentioning

confidence: 96%

“…Basophil count was not reported in this study as it was shown to be unreliable in canine specimens measured with the XT‐2000iV …”

Section: Methodsmentioning

confidence: 99%

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Changes in hematology measurements in healthy and diseased dog blood stored at room temperature for 24 and 48 hours using theXT‐2000iV analyzer

Bourgès‐Abella

Geffré

Deshuillers

et al. 2014

Veterinary Clinical Pathol

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Background Changes in canine hematology measurements may occur when analyses are delayed due to shipment of specimens to a laboratory. Objective The purpose of this study was to report changes in hematologic variables in healthy and diseased canine blood measured with a Sysmex XT‐2000iV during storage at room temperature for 24 and 48 hours. Methods EDTA‐K3 blood samples from 42 healthy and diseased dogs were measured on a Sysmex XT‐2000iV analyzer within one hour of sampling, and after storage for 24 and 48 hours at room temperature in the dark. Results Storage caused little or no change in RBC count, HGB concentration and MCH, while there was a moderate increase in HCT, MCV and reticulocytes count, and a moderate decrease in MCHC. Decreased platelet counts by impedance (PLT‐I) and optical (PLT‐O) measurements were associated with increased mean platelet volume (MPV), platelet‐large cell ratio (P‐LCR) and platelet distribution width (PDW), including a right shift in the platelet histogram and a dispersion of the platelet dot plot on the scattergram. The total and differential WBC count remained stable except for decreased monocyte counts. In the scatterplots, monocytes shifted into the lymphocyte population after 24 hours, and neutrophil population shifted to the right appearing in the eosinophil gate at 48 hours of storage. The disease status had only a small effect on storage‐induced changes, and observed changes had no consequences for clinical decisions. Conclusions Blood storage at room temperature was accompanied by moderate variations in some hematologic variables, awareness of which helps in avoiding misinterpretations.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 96%

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Changes in hematology measurements in healthy and diseased dog blood stored at room temperature for 24 and 48 hours using theXT‐2000iV analyzer

Bourgès‐Abella

Geffré

Deshuillers

et al. 2014

Veterinary Clinical Pathol

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“…Samples were analyzed first with the ADVIA 120 (Siemens Healthcare Diagnostics Inc., Deerfield, IL, USA) and then with the Sysmex XT‐2000iV (Sysmex Corporation, Kobe, Japan). Both instruments use multispecies software previously validated for dogs …”

Section: Methodsmentioning

confidence: 99%

Grey eosinophils in sighthounds: frequency in 3 breeds and comparison of eosinophil counts determined manually and with 2 hematology analyzers

Giori

Gironi

Scarpa

et al. 2011

Veterinary Clinical Pathol

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“…11 The evaluation of linearity in the current study is limited by the fact that the preparation of a platelet-rich blood sample was not performed as it would have required a large blood volume. A previous study, however, reported good linearity for a leukocyte count up to 100 × 10 9 /l and a PLT up to 4,140 × 10 9 /l for feline specimens.…”

mentioning

confidence: 99%

Evaluation of the automated hematology analyzer Sysmex XT-2000iV™ compared to the ADVIA® 2120 for its use in dogs, cats, and horses

et al. 2011

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The automated laser-based hematology analyzer Sysmex XT-2000iV™ providing a complete blood cell count (CBC) and 5-part differential has been introduced in large veterinary laboratories. The aim of the current study was to determine precision, linearity, and accuracy of the Sysmex analyzer. Reference method for the accuracy study was the laser-based hematology analyzer ADVIA® 2120. For evaluation of accuracy, consecutive fresh blood samples from healthy and diseased cats (n = 216), dogs (n = 314), and horses (n = 174) were included. A low intra-assay coefficient of variation (CV) of approximately 1% was seen for the CBC except platelet count (PLT). An intra-assay CV ranging between 2% and 5.5% was evident for the differential count except for feline and equine monocytes (7.7%) and horse eosinophils (15.7%). Linearity was excellent for white blood cell count (WBC), hematocrit value, red blood cell count (RBC), and PLT. For all evaluated species, agreement was excellent for WBC and RBC, with Spearman rank correlation coefficients (r(s)) ranging from >0.99 to 0.98. Hematocrit value correlated excellently in cats and dogs, whereas for horses, a good correlation was evident. A good correlation between both analyzers was seen in feline and equine PLT (r(s) = 0.89 and 0.92, respectively), whereas correlation was excellent for dogs (r(s) = 0.93). Biases were close to 0 except for mean corpuscular hemoglobin concentration (4.11 to -7.25 mmol/l) and canine PLT (57 × 10(9)/l). Overall, the performance of the Sysmex analyzer was excellent and compared favorably with the ADVIA analyzer.

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Evaluation of the Sysmex XT-2000iV haematology analyser for rat, dog and mouse whole blood samples

Cited by 24 publications

References 6 publications

Changes in hematology measurements in healthy and diseased dog blood stored at room temperature for 24 and 48 hours using theXT‐2000iV analyzer

Changes in hematology measurements in healthy and diseased dog blood stored at room temperature for 24 and 48 hours using theXT‐2000iV analyzer

Grey eosinophils in sighthounds: frequency in 3 breeds and comparison of eosinophil counts determined manually and with 2 hematology analyzers

Evaluation of the automated hematology analyzer Sysmex XT-2000iV™ compared to the ADVIA® 2120 for its use in dogs, cats, and horses

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