Evaluation of the rapid RIDAQUICK Campylobacter® test in a general hospital

Gómez-Camarasa, Cristina; Gutiérrez‐Fernández, José; Rodríguez‐Grangér, Javier; Sampedro-Martínez, Antonio; Sorlózano-Puerto, Antonio; Navarro-Marí, José María

doi:10.1016/j.diagmicrobio.2013.11.009

Cited by 11 publications

(6 citation statements)

References 21 publications

(23 reference statements)

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“…An only slightly lower PPA (87.1%; 95% CI, 70.5-95.5%) of the same ICA with culture/PCR than reported by us can be calculated for data recently published from Spain [9]. This difference may be due to distinct patient populations or different transport conditions and/or times.…”

Section: Discussionsupporting

confidence: 45%

“…Although confi rmation by PCR would have been desirable but unfortunately not feasible, some ICA-positive but culture-negative samples in our study might have been true positives as previously shown for both the immunoCard STAT! and the RidaQuick assay [7,9], although also false-positive results have been reported for both assays [9,13,14]. The new generation of the EIA tested has been improved compared with the previous type, which only had a sensitivity of 69% and a specifi city of 87% compared with culture [6].…”

Section: Discussionmentioning

confidence: 99%

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Accurate detection ofCampylobacterspp. antigens by immunochromatography and enzyme immunoassay in routine microbiological laboratory

Regnath¹,

Ignatius²

2014

European Journal of Microbiology and Immunology

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Campylobacter spp. are fastidious microorganisms, and their detection by culture depends on the freshness of the stool sample and the skills of the laboratory staff. To improve laboratory diagnosis, assays for the detection of specific antigens have been developed. Here, we evaluated two assays for the detection of Campylobacter spp.-specific antigens, i.e., one immunochromatographic test and one enzyme-linked immunosorbent assay (EIA), in 38 frozen Campylobacter spp.-positive specimens and prospectively in 533 fresh stool samples with a conventional enzyme immunoassay (EIA) and culture.Both assays were positive for 36 samples with Campylobacter jejuni and one with Campylobacter coli among 38 Campylobacter spp.-positive frozen samples. One Campylobacter lari-positive sample was identified by the immunochromatographic assay (ICA) only. In a prospective study performed within the course of routine microbiology, both assays were positive for 24/25 C. jejuni culture-positive samples (positive percent agreement, 96.0% [95% CI: 78.9-100%]). ICA and EIA also were positive for 14 and 10 culture-negative samples, respectively (negative percent agreement: ICA, 97.2% [95% CI: 95.4-98.4%]; EIA, 98.0% [95% CI: 96.4-99.0%]). In conclusion, the high agreement between both antigen-detection assays and culture indicates that both assays may be initially performed followed by culture only upon a positive test result.

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Section: Discussionsupporting

confidence: 45%

Section: Discussionmentioning

confidence: 99%

Accurate detection ofCampylobacterspp. antigens by immunochromatography and enzyme immunoassay in routine microbiological laboratory

Regnath¹,

Ignatius²

2014

European Journal of Microbiology and Immunology

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“…Our study shows a very interesting performance of the ICTs in terms of PPV and NPV compared to previous studies (9, 10). One prior study evaluated the Ridaquick Campylobacter performance and obtained equivalent results in terms of specificity (97%) but reported a higher sensitivity (87%) (15). Differences can be explained by the fact that they did not use a CRS, only a molecular test when culture and the Ridaquick Campylobacter were discordant.…”

Section: Discussionmentioning

confidence: 98%

Evaluation of the Diagnostic Accuracy of Two Immunochromatographic Tests Detecting Campylobacter in Stools and Their Role in Campylobacter Infection Diagnosis

et al. 2018

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The detection of campylobacters in stools is performed essentially by culture, but this technique has a low sensitivity. New detection methods are now available. Among them, immunochromatography tests (ICTs) are very attractive in that they offer a result within 15 min. However, previous studies suggest that these tests have a relatively low specificity. The objective of this study was to evaluate the performance of these tests. During the study period, all patients who consulted the emergency units and had a stool culture were included. Their stool samples were tested with two ICTs, Ridaquick Campylobacter and Immuno STAT! Campy. Stools were also tested by a home-made PCR and two commercially available enzyme-linked immunosorbent assays (ELISAs) when one of the ICTs was positive. The composite reference standard (CRS) was defined as positive if the culture was positive or, in case of a negative culture, if the PCR and one of the ELISAs were positive simultaneously. Three hundred and five patients were included. Among the 50 positive specimens with Ridaquick Campylobacter, 47 were considered true positives by the CRS, corresponding to a positive predictive value (PPV) of 94.0%. Among the 52 positive specimens with Immuno STAT! Campy, 44 were considered true positives by the CRS, corresponding to a PPV of 84.6%. The negative predictive values were estimated at 94.9 and 92.4% for the Ridaquick Campylobacter and Immuno STAT! Campy tests, respectively. ICTs appear to be very efficient and allow a very rapid detection of campylobacters, which is important for treating early campylobacter infections with an adapted antibiotherapy.

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“…In a another study by Zaghloul et al,(2012) [45] in Ain shams,Egypt,the sensitivity of IC was 86.9%, specificity 98.7%, positive and negative predictive values were 83.3% and 99.7%, respectively.…”

Section: Awadallah Et Al (2014)mentioning

confidence: 85%

Immunochromatography versus Conventional Culture Method for Detection of Campylobacter Jejuni and Campylobacter Coli in Gastroenteritis

Arafa

Youssef

Tabl

et al. 2017

EJMM

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Campylobacter jejuni (C.jejuni) , Campylobacter coli (C.coli), modified charcoal cefoperazol dextrose agar(MccDA) Background:Campylobacter is an important food-borne diarrheal disease in the whole world. The use of cultureindependent diagnostic tests , such as stool antigen tests, for the detection of Campylobacter in stool is of particular interest.Objectives:This study aimed to compare between conventional culture method and Immunochromatography (I.C) For Detection Of C.jejuni and C. coli in Gastroenteritis at Benha University hospitals, and determine their antibiotic susceptibility pattern.Methodology: This study was carried out on 100 patients who developed gastroenteritis like symptoms attending the out patient clinic of Benha University Hospitals or admitted to the Pediatric and Internal medicine departments. Stool samples were cultured on modified charcoal cefoperazol dextrose agar (mCCDA) and were examined for Ag detection by I.C test using RIDA®QUICK kit .Antimicrobial susceptibility testing was performed for twelve different antibiotics by the Kirby-Bauer disc diffusion method. Results: Campylobacter was detected in 18% of samples by culture method. 72.2% of them were C. jejuni, while the other 27.8% were C. coli. Out of 18 positive by culture ,16(88.9%) were positive by I.C. However, out of 82 negative by culture , 2(2.4%) were positive by I.C. The diagnostic accuracy values of the RidaQuick Campylobacter® versus culture were: sensitivity of 88.9%, specificity of 97.6%, and positive and negativepredictive values of 88.9% and 97.6%, respectively. Conclusion: RIDAQUICK Campylobacter®provide a rapid and reliable alternative for conventional culture in laboratory diagnosis of enteric infections with C. jejuni and C. coli.

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Evaluation of the rapid RIDAQUICK Campylobacter® test in a general hospital

Cited by 11 publications

References 21 publications

Accurate detection ofCampylobacterspp. antigens by immunochromatography and enzyme immunoassay in routine microbiological laboratory

Accurate detection ofCampylobacterspp. antigens by immunochromatography and enzyme immunoassay in routine microbiological laboratory

Evaluation of the Diagnostic Accuracy of Two Immunochromatographic Tests Detecting Campylobacter in Stools and Their Role in Campylobacter Infection Diagnosis

Immunochromatography versus Conventional Culture Method for Detection of Campylobacter Jejuni and Campylobacter Coli in Gastroenteritis

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