Evaluation of the Diagnostic Accuracy of Two Immunochromatographic Tests Detecting Campylobacter in Stools and Their Role in Campylobacter Infection Diagnosis

Bessède, Emilie; Asselineau, Julien; Perez, Paul; Valdenaire, Guillaume; Richer, Olivier; Lehours, Philippe; Mégraud, Françis

doi:10.1128/jcm.01567-17

Cited by 14 publications

(12 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…culture, only double-positive cases by two independent PCRs could be considered as true Campylobacter sp. positives in case of negative culture [2,8,9]. We did not find a perfect correlation between the Campylobacter PCR results obtained on BD MAX TM and the two independent PCR formats.…”

Section: Discussioncontrasting

confidence: 65%

“…The culture of Campylobacter sp. can unfortunately be falsely negative in 10% to 30% of cases [2] due to preanalytical and analytical factors. Clinical laboratories equipped with syndromic polymerase chain reaction (PCR) systems in the context of a bacterial infectious diarrhea diagnosis are tempted to use only selective agar for the pathogen(s) detected by PCR.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

How to Interpret a Positive Campylobacter PCR Result Using the BD MAXTM System in the Absence of Positive Culture?

Gueudet

Paolini²,

Buissonnière

et al. 2019

JCM

Self Cite

View full text Add to dashboard Cite

The aim of this study was to evaluate, using two independent polymerase chain reaction (PCR) formats, the results of Campylobacter detection by the BD MAXTM Enteric Bacterial Panel PCR (Becton Dickinson, Le Pont de Claix, France) in the absence of positive culture. A total of 77 samples found positive for Campylobacter on BD MAXTM but negative by culture were studied. Upon reception, one in-house real-time-PCR for Campylobacter sp. and a PCR with the RIDAGENE Bacterial Stool Panel (r-biopharm, Darmstadt, Germany) were performed. The data obtained using these two PCR formats were evaluated with respect to the cycle threshold (Ct) and fluorescence intensity values (FI) obtained on BD MAXTM. Ct and FI values were also obtained for 80 positive Campylobacter cases by culture. Among the 77 samples, 33 were positive with the two PCRs, and 37 remained negative. For the 33 double-positive PCRs samples, the Ct values obtained on BD MAXTM were lower than 30 in 93.9%, and FI > 2000 for 97% of cases. For the 37 double-negative PCRs samples, the Ct values obtained on BD MAXTM were <30 in only 18.9%, however FI were >2000 for 40.5% of cases. Positive culture cases had Ct values < 30 in 96.2% and FI > 2000 in 98.8%. We showed that the Ct values obtained on BD MAXTM can help to interpret the results. Almost 96% of the Campylobacter sp. cases detected by culture or with the two reference PCRs positive showed a Ct value on BD MAXTM, meaning that stools detected as positive with BD MAXTM and having a Ct > 30 may be false positives.

show abstract

Section: Discussioncontrasting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

How to Interpret a Positive Campylobacter PCR Result Using the BD MAXTM System in the Absence of Positive Culture?

Gueudet

Paolini²,

Buissonnière

et al. 2019

JCM

Self Cite

View full text Add to dashboard Cite

show abstract

“…Culture of Campylobacter spp. is particularly troublesome, with sensitivity reported to range from 60−76% 19,20 , and as evident from its ~30% rate of failure to detect true-positive specimens here. Personnel can expect that control EIA and molecular tests will frequently produce positive results when culture data are negative.…”

Section: Discussionmentioning

confidence: 83%

Culture Methods to Determine the Limit of Detection and Survival in Transport Media of <em>Campylobacter Jejuni</em> in Human Fecal Specimens

Buss

Thacker

Santiago

2020

JoVE

View full text Add to dashboard Cite

A culture from human stool for diagnosis of Campylobacter-based intestinal illness takes several days, a wait that taxes the fortitude of the physician and the patient. A culture is also prone to false negative results from random loss of viability during specimen handling, overgrowth of other fecal flora, and poor growth of several pathogenic Campylobacter species on traditional media. These problems can confound clinical decisions on patient treatment and have limited the field from answering fundamental questions on Campylobacter growth and infections. We describe a procedure that estimates the lower limit of bacterial numbers that can be detected by a culture and a method for quantifying survival of C. jejuni in media used for transport of this fragile organism. Knowing this information, it becomes possible to set clinically relevant detection thresholds for diagnostic tests and address unstudied issues of whether non-symptomatic colonization is prevalent, if co-infection with other enteric pathogens is common, or if bacterial load correlates with symptoms or serious sequelae. The study also included testing of 1,552 prospectively collected patient diarrheal fecal specimens that were initially classified by conventional culture and further tested by a new enzyme immunoassay. Positive and discrepant specimens were then screened by four molecular methods to assign true-positive or true-negative status. The 5 non-culture methods showed complete agreement on all 48 positive and discrepant specimens, while the culture mis-identified 14 (28%). The specimens that were incorrectly identified by culture included 13 false negative and 1 false positive sample. This basic protocol can be used with multiple Campylobacter spp. and will allow the numbers of Campylobacter bacteria that produce symptoms of gastroenteritis in humans to be determined and for prevalence rates to be updated.

show abstract

“…There are also nonculture-based methods for the direct detection of campylobacters in human or animal faeces and processed food samples, which allow the identification of this fastidious organism without the specialized media and equipment needed for Campylobacter culture. Several enzyme immunoassays (EIA), which are based on antigenantibody interaction, have been developed for this purpose in human faeces and are commercially available in a form of kits (Bessède et al, 2018;Dediste et al, 2003;Granato et al, 2010;Tolcin et al, 2000). While the culture-independent diagnostic tests (CIDTs) are convenient to use, the sensitivity, specificity, and positive predictive value of Campylobacter stool antigen tests have found to be highly variable (Bessède et al, 2011;Giltner et al, 2013;Granato et al, 2010) and therefore their use as standalone tests for direct detection of Campylobacter in stool is questioned.…”

Section: Direct Detection Methodsmentioning

confidence: 99%

The genus Campylobacter: detection and isolation methods, species identification & typing techniques

Natsos

Mouttotou

Ahmad³

et al. 2019

J Hellenic Vet Med Soc

View full text Add to dashboard Cite

Campylobacter is well recognized as the leading cause of bacterial foodborne diarrheal disease worldwide; while, poultry has been identified as a significant cause of campylobacter infection in humans. The C. jejuni has been found to be the predominant species isolated from poultry samples and, yet, responsible for the majority of human campylobacteriosis. Campylobacter spp. are small, oxidase positive, microaerophilic, curved gram-negative rods exhibiting corkscrew motility and colonize the intestinal tract of most mammalian and avian species. From its very first description in late 19th century by Theodor Escherich until nowadays, a lot of research has been carried out providing a wealth of information regarding its microbiological properties. Since novel technologies constantly emerge, increasingly advanced methods for detection, identification and typing of Campylobacter spp. are becoming available. The aim of this article is to review the recent bibliography on Campylobacter focusing, especially, on its survival and growth characteristics, the laboratory methods used for its detection and isolation from clinical, animal, environmental, and food samples, the reported methods applied for its speciation, as well as the typing systems developed for subtyping of Campylobacter.

show abstract

Evaluation of the Diagnostic Accuracy of Two Immunochromatographic Tests Detecting Campylobacter in Stools and Their Role in Campylobacter Infection Diagnosis

Cited by 14 publications

References 16 publications

How to Interpret a Positive Campylobacter PCR Result Using the BD MAXTM System in the Absence of Positive Culture?

How to Interpret a Positive Campylobacter PCR Result Using the BD MAXTM System in the Absence of Positive Culture?

Culture Methods to Determine the Limit of Detection and Survival in Transport Media of <em>Campylobacter Jejuni</em> in Human Fecal Specimens

The genus Campylobacter: detection and isolation methods, species identification & typing techniques

Contact Info

Product

Resources

About