Accurate detection of<i>Campylobacter</i>spp. antigens by immunochromatography and enzyme immunoassay in routine microbiological laboratory

Regnath, T; Ignatius, Ralf

doi:10.1556/eujmi-d-14-00018

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…Additionally, as previously mentioned, repeatability is important. In the case of EIA assays, Regnath and Ignatius (2014) also observed a similar agreement to Granato and colleagues when comparing EIA and culture methods for the detection of C. jejuni and coli from stool samples.…”

Section: Immune-based Assays For Campylobactersupporting

confidence: 78%

Developments in Rapid Detection Methods for the Detection of Foodborne Campylobacter in the United States

et al. 2019

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The accurate and rapid detection of Campylobacter spp. is critical for optimal surveillance throughout poultry processing in the United States. The further development of highly specific and sensitive assays to detect Campylobacter in poultry matrices has tremendous utility and potential for aiding the reduction of foodborne illness. The introduction and development of molecular methods such as polymerase chain reaction (PCR) have enhanced the diagnostic capabilities of the food industry to identify the presence of foodborne pathogens throughout poultry production. Further innovations in various methodologies, such as immune-based typing and detection as well as high throughput analyses, will provide important epidemiological data such as the identification of unique or region-specific Campylobacter. Comparable to traditional microbiology and enrichment techniques, molecular techniques/methods have the potential to have improved sensitivity and specificity, as well as speed of data acquisition. This review will focus on the development and application of rapid molecular methods for identifying and quantifying Campylobacter in U.S. poultry and the emergence of novel methods that are faster and more precise than traditional microbiological techniques.

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Section: Immune-based Assays For Campylobactersupporting

confidence: 78%

Developments in Rapid Detection Methods for the Detection of Foodborne Campylobacter in the United States

et al. 2019

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“…Additionally, a real-time PCR (qPCR) is a preferable assay for rapid real-time quantification and identification of this bacteria [25][26][27]. Furthermore, several enzyme immunoassays are also available for the screening of C. jejuni in clinical samples [28][29][30].…”

Section: Introductionmentioning

confidence: 99%

A Cutoff Determination of Real-Time Loop-Mediated Isothermal Amplification (LAMP) for End-Point Detection of Campylobacter jejuni in Chicken Meat

Jainonthee

Chaisowwong

Ngamsanga

et al. 2022

Veterinary Sciences

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Campylobacter jejuni is one of the leading causes of foodborne illness worldwide. C. jejuni is commonly found in poultry. It is the most frequent cause of contamination and thus resulting in not only public health concerns but also economic impacts. To test for this bacterial contamination in food processing plants, this study attempted to employ a simple and rapid detection assay called loop-mediated isothermal amplification (LAMP). The best cutoff value for the positive determination of C. jejuni calculated using real-time LAMP quantification cycle (Cq) was derived from the receiver operating characteristic (ROC) curve modeling. The model showed an area under curve (AUC) of 0.936 (95% Wald CI: 0.903–0.970). Based on Youden’s J statistic, the optimal cutoff value which had the highest sensitivity and specificity from the model was calculated as 18.07. The LAMP assay had 96.9% sensitivity, 95.8% specificity, and 93.9 and 97.9% positive and negative predictive values, respectively, compared to a standard culture approach for C. jejuni identification. Among all non-C. jejuni strains, the LAMP assay gave each of 12.5% false-positive results to C. coli and E. coli (1 out of 8 samples). The assay can detect C. jejuni at the lowest concentration of 103 CFU/mL. Our results suggest a preliminary indicator for the application of end-point LAMP assays, such as turbidity and UV fluorescence tests, to detect C. jejuni in field operations. The LAMP assay is an alternative screening test for C. jejuni contamination in food samples. The method provides a rapid detection, which requires only 9 min with a cutoff value of Cq. We performed the extraction of DNA from pure cultures and the detection of C. jejuni using the LAMP assay within 3 h. However, we were not able to reduce the time for the process of enrichment involved in our study. Therefore, we suggest that alternative enrichment media and rapid DNA extraction methods should be considered for further study. Compared to other traditional methods, our proposed assay requires less equipment and time, which is applicable at any processing steps in the food production chain.

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Gastrointestinal Infections

2017

Clinical Microbiology for Diagnostic Laboratory Scientists

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Accurate detection ofCampylobacterspp. antigens by immunochromatography and enzyme immunoassay in routine microbiological laboratory

Cited by 3 publications

References 18 publications

Developments in Rapid Detection Methods for the Detection of Foodborne Campylobacter in the United States

Developments in Rapid Detection Methods for the Detection of Foodborne Campylobacter in the United States

A Cutoff Determination of Real-Time Loop-Mediated Isothermal Amplification (LAMP) for End-Point Detection of Campylobacter jejuni in Chicken Meat

Gastrointestinal Infections

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