Evaluation of the phytochemical composition and protective activities of methanolic extracts of Centaurea borysthenica and Centaurea daghestanica (Lipsky) Wagenitz on cardiomyocytes treated with doxorubicin

Abstract

Centaurea L. is a genus of the family Asteraceae that comprises over 600 taxa. Representatives of the Centaurea genus were used as natural medications for many diseases. Methanolic-aqueous extracts from aerial parts of two Centaurea species: C. borysthenica Gruner and C. daghestanica (Lipsky) Wagenitz were studied for their polyphenolic composition and potential protective effect on cardiomyocytes treated with doxorubicin. Effectiveness of doxorubicin in cancer therapy is limited by a dose-dependent cardiotoxicity. Oxidative stress is a widely recognized mechanism of this phenomenon. One of the most important strategies has been an application of drug together with antioxidant agents. A cardioprotective effect of selected extracts of Centaurea species was suspected in this study. Cell viability, oxidative stress, and mitochondrial membrane potential analyses showed protective activity of the methanolic extract of C. borysthenica and C. daghestanica on rat cardiomyocytes treated with doxorubicin. Although C. borysthenica is more effective as a cardiomyocyte protective agent, in higher concentrations it weakened the drug activity. C. daghestanica extract did not change the doxorubicin efficacy in the evaluated experiment. Interestingly, both tested extracts were cytotoxic for myeloma cells. The detected antioxidant activity of the studied extracts can be used in the prevention of doxorubicin-induced cardiotoxicity.

Flavonoids and Other Phenolic Compounds from Medicinal Plants for Pharmaceutical and Medical Aspects: An Overview

“…The obtained results from oxidative stress, cell viability, and mitochondrial membrane potential tests displayed their cardioprotective activity of both C. borysthenica and C. daghestanica extracts on rat cardiomyocytes treated with doxorubicin anticancer drug. According to this study, they found an attractive point that C. daghestanica methanolic extracts did not affect on efficacy of doxorubicin in this experiment [ 53 ].…”

“…The cardioprotective effects from various kinds of phenolics and/or flavonoids occurring in medicinal plants have been investigated from many researches since many decades ago [ 1 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 ]. The comprehensive review of Razavi-Azarkhiavi and his team illustrated cardioprotective role of various phenolic compounds against cardiotoxicity of doxorubicin which is the extensively used anticancer medicine for lymphomas, leukemia and breast cancers in clinical application that contains vulnerable side effect as cardiotoxicity such as pericarditis, arrhythmias, myocarditis, and acute heart failure [ 52 ].…”

“…( ] were analyzed together with their protective effects on cardiomyocytes treated with doxorubicin [ 53 ]. The obtained results from oxidative stress, cell viability, and mitochondrial membrane potential tests displayed their cardioprotective activity of both C. borysthenica and C. daghestanica extracts on rat cardiomyocytes treated with doxorubicin anticancer drug.…”

An assessment of phenolic profiles, fatty acid compositions, and biological activities of twoHelichrysumspecies:H. plicatumandH. chionophilum

“…The bioactive compounds obtained from plants have been used as the main resource of natural products recently (Babotă et al, ; Bahadori, Kirkan, & Sarikurkcu, ; Korga et al, ). The herbal products become very popular especially because the uses of synthetic drugs have several concerns (Zengin et al, ).…”

Cytotoxic activity of methanolic fractions of different Marrubium spp. against melanoma cells is independent of antioxidant activity and total phenolic content

“…FA, FB and FC fractions obtained from M. vulgare, M. friwaldskyanum and M. cylleneum were added to the cell cultures in the following final concentrations: 200, 100, 50, 25 and 12.5 µgÁmL À1 . Further measurement was performed as previously described [27]. One percent Triton X-100 was used as a positive control for the MTT assay.…”