Evaluation of the phosphate solubilization potential of trichoderma strains (trichoplus jco) and effects on rice biomass

Abstract

This study aims to evaluate the phosphate solubilization potential of the Trichoderma spp. isolates found in the commercial product Trichoplus JCO. The strains were isolated, molecularly identified and grown in vitro. The soluble phosphate concentration was quantified at 3, 6, 9 and 12 days after transplanting. Rice was inoculated with the Trichoderma strains in greenhouse conditions with 10 to 60 x10 8 conidia per gram of rice and then planted. At 30 and 45 days after planting (DAP), plant growth characteristics were determined. The phosphate concentrations of most of the Trichoderma strains showed a general trend of increasing from days 3 to 9. At 30 DAP, Tr. Harz and Tr. TSM had a higher significant difference. At 45 DAP, the rice inoculated with Tr. Harz, Tr. Euc and Tr. TSM had the greatest increase in the total dry matter compared with the control. The rice inoculated with Tr. Euc and Tr. TSM had the best relative efficiencies, and the rice inoculated with Tr. Din and Tr. TSM demonstrated the highest P-UEF. Finally, the rice inoculated with Tr. Harz (T. asperelloides), Tr. Euc (T. harzianum) and Tr. TSM (T. harzianum) had the highest efficiency in phosphate solubilization with increases in rice biomass.

Phylogenetic Diversity of Trichoderma Strains and Their Antagonistic Potential against Soil-Borne Pathogens under Stress Conditions

“…Our results proved that both T. asperellum strains (Ta1 and Ta2) had a higher phosphate solubilization index (6 and 8 mm, respectively) as per a previous report [78] that T. asperellum Q1 was able to produce phosphatase enzymes for phosphate solubilization under salt stress conditions. Similar results were also recorded in another study [8], which found that T. asperelloides and T. harzianum were the highest phosphate solubilizers of five strains studied and suggested that Trichoderma strains used the solubilized phosphate in their cellular processes. These results were logical as it has been reported [79] that fungi have a large capability for solubilizing rock phosphate.…”

“…The assay was modified from previously described method [8,56] to test the ability of Trichoderma strains for phosphate solubilization. Briefly, each strain was singly cultured on a Petri dish (100 mm) containing 10 mL of NBRIP agar composed of 10.0 g/L glucose,…”

“…This means that Trichoderma used its ability for faster growth as an essential mechanism to compete with pathogenic fungi. Moreover, rapid growth rate helps Trichoderma strains to compete with pathogens by solubilizing phosphate faster to facilitate their mycelial growth [8]. In comparison, most plant pathogenic fungi are incapable of solubilizing phosphate.…”

Phosphorus-solubilizing Trichoderma spp. from Amazon soils improve soybean plant growth

“…According to Cornejo, Trichoderma enhances the lateral roots instead of the formation of new roots. Many authors have reported that during the process of P solubilization, the pH of the medium becomes acidified, probably due to the production of organic acids 30,[50][51][52] . Thus, a correlation between the decrease in pH and the increase in P solubilization influences the biomass increase of the lateral roots 12 and consequently increases the surface of P absorption by plants.…”

Seedling root-dip in phosphorus and biofertilizer added soil slurry method of nutrient management for transplanted rice in acid soil

“…These results were supported by the past finding that RP maintained higher amount of AvlP in soil than SSP (Mandal and Khan, 1972). Again, the combined application of RP and PSB further increased soil AvlP compared to their individual application (Thakuria et al, 2009;Borges Chagas et al, 2015). Thus, it seems that PSB+RP and SRD in SSP Soil slurry+PSB+RP application could maintain a synchrony between the supply of soil AvlP and the rate of P uptake in plant over the crop growing period.…”