This study aims to evaluate the phosphate solubilization potential of the Trichoderma spp. isolates found in the commercial product Trichoplus JCO. The strains were isolated, molecularly identified and grown in vitro. The soluble phosphate concentration was quantified at 3, 6, 9 and 12 days after transplanting. Rice was inoculated with the Trichoderma strains in greenhouse conditions with 10 to 60 x10 8 conidia per gram of rice and then planted. At 30 and 45 days after planting (DAP), plant growth characteristics were determined. The phosphate concentrations of most of the Trichoderma strains showed a general trend of increasing from days 3 to 9. At 30 DAP, Tr. Harz and Tr. TSM had a higher significant difference. At 45 DAP, the rice inoculated with Tr. Harz, Tr. Euc and Tr. TSM had the greatest increase in the total dry matter compared with the control. The rice inoculated with Tr. Euc and Tr. TSM had the best relative efficiencies, and the rice inoculated with Tr. Din and Tr. TSM demonstrated the highest P-UEF. Finally, the rice inoculated with Tr. Harz (T. asperelloides), Tr. Euc (T. harzianum) and Tr. TSM (T. harzianum) had the highest efficiency in phosphate solubilization with increases in rice biomass.
The objective of this study was to evaluate the efficiency of Bacillus subtilis inoculation in the agronomic performance of the soybean cv. M 9144 RR crop under field conditions. Two field experiments were developed with inoculation by B. subtilis in soybeans in two regions. The experiments were conducted in 2015/2016 harvest from December to April. The experiment was performed in a randomized block design with four replicates with and without inoculation by B. subtilis. Consequently, biomass and productivity were determined. The B. subtilis inoculation increased soybean biomass in both assessment regions. This bacterium showed increased soybean yield in Gurupi and Araguaçu soils (Tocantins, Brazil), with increases above 14.9 and 8.8%, respectively. The inoculation of the strain B. subtilis UFT-Bs10 provided biomass increase, nodulation, stand maintenance and increased yield of soybean cv. M 9144 RR in field conditions.
Filamentous fungi of the genus Trichoderma are known for their activity as plant growth promoters. The objective of this work was to evaluate the efficiency of the Trichoderma asperellum UFT 201 strain as a plant growth promoter in soybean. The ability of phosphate solubilization, indole acetic acid synthesis and the effect on soybean plant growth under greenhouse conditions were evaluated in four experiments. In vitro and in vivo analyzes were performed. Plant biomass characteristics such as shoot dry mass (SDM), root dry mass (RDM) and total dry mass (TDM) were evaluated. T. asperellum UFT 201 showed a significantly higher concentration (p <0.05) with a phosphate solubilization capacity of 67.8% higher than the positive control treatment (Trichoderma harzianum). The production of indole acetic acid (IAA) by T. asperellum UFT 201 was 26.7% higher than the positive control. The effect on the biomass treated by T. asperellum UFT 201 was higher (p <0.05) than the positive and absolute controls. Thus, inoculation by T. asperellum UFT 201 showed high efficiency as a growth promoter in soybean.
The goal of this study was to evaluate the combined inoculation of rhizobia and Trichoderma spp. in cowpea, their ability to promote growth and use as a biological control agent for foliar blight (Rhizoctonia solani). The study was conducted in a field experiment in a randomized block design with four replications. The treatments consisted of seed-applied rhizobia with and without Trichoderma spp., and to the soil at planting time and 15 days after planting (DAP). The rhizobia inoculation was performed with a mixture of strains INPA 03-11B and UFLA 03-84. For the treatments with Trichoderma spp., a commercial powder product, Trichoplus JCO was used. The application of Trichoderma spp. and rhizobia at 15 DAP, in both the seed and in the soil, showed the best results (p < 0.05). The application of Trichoderma spp. did not inhibit the nodulation process, and the best results were obtained when the fungus was inoculated with rhizobia. The inoculation treatments were positive for Trichoderma spp. The Trichoderma spp. treatments showed improvements in stand counts, survival and effectiveness against R. solani, reinforcing that the usage of Trichoderma spp. as a seed and soil treatment.
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