Evaluation of the Phoenix Automated Microbiology System for Detecting Extended-Spectrum β-Lactamase in Escherichia coli, Klebsiella species and Proteus mirabilis

Lee, Kyo Kwan; Kim, Sung Tae; Hong, Ki Suk; Huh, Hee Jin; Chae, Seok-Lae

doi:10.3343/kjlm.2008.28.3.185

Cited by 4 publications

(2 citation statements)

References 15 publications

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“…Therefore, many automated identification and susceptibility testing systems are available (3,11). These can identify microorganisms and determine antibiotic susceptibility in hours; however, they require pure colonies, making overnight subculture of positive blood cultures inevitable.…”

Section: Discussionmentioning

confidence: 99%

Development and Evaluation of Oligonucleotide Chip Based on the 16S-23S rRNA Gene Spacer Region for Detection of Pathogenic Microorganisms Associated with Sepsis

et al. 2010

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Oligonucleotide chips targeting the bacterial internal transcribed spacer region (ITS) of the 16S-23S rRNA gene, which contains genus-and species-specific regions, were developed and evaluated. Forty-three sequences were designed consisting of 1 universal, 3 Gram stain-specific, 9 genus-specific, and 30 species-specific probes. The specificity of the probes was confirmed using bacterial type strains including 54 of 52 species belonging to 18 genera. The performance of the probes was evaluated using 825 consecutive samples that were positive by blood culture in broth medium. Among the 825 clinical specimens, 708 (85.8%) were identified correctly by the oligonucleotide chip. Most (536 isolates, or 75.7%) were identified as staphylococci, Escherichia coli, or Klebsiella pneumoniae. Thirty-seven isolates (4.5%) did not bind to the corresponding specific probes. Most of these also were staphylococci, E. coli, or K. pneumoniae and accounted for 6.3% of total number of the species. Sixty-two specimens (7.5%) did not bind the genus-or species-specific probes because of lack of corresponding specific probes. Among them, Acinetobacter baumannii was the single most frequent isolate (26/62). The oligonucleotide chip was highly specific and sensitive in detecting the causative agents of bacteremia directly from positive blood cultures.

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Section: Discussionmentioning

confidence: 99%

Development and Evaluation of Oligonucleotide Chip Based on the 16S-23S rRNA Gene Spacer Region for Detection of Pathogenic Microorganisms Associated with Sepsis

et al. 2010

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“…İzole edilen bakterilerin identifikasyonu ve antibiyotik duyarlılık-ları PHOENİX (Becton Dickinson Diagnostic Instrument System, Spark Md, USA) otomatize sistemde yapılmıştır (9,10) . (11) .…”

Section: Gereç Ve Yöntemunclassified

A Comparison of The Resistance Ratio Obtained in The Three Different Time Intervals in Escherichia coli Strains Against Ciprofloxacin, Cefotaxime and Imipenem

Atmaca¹,

Özekinci²,

Özcan³

et al. 2016

IKSST

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Expert Systems in Clinical Microbiology

Winstanley

Courvalin

2011

Clin Microbiol Rev

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SUMMARY This review aims to discuss expert systems in general and how they may be used in medicine as a whole and clinical microbiology in particular (with the aid of interpretive reading). It considers rule-based systems, pattern-based systems, and data mining and introduces neural nets. A variety of noncommercial systems is described, and the central role played by the EUCAST is stressed. The need for expert rules in the environment of reset EUCAST breakpoints is also questioned. Commercial automated systems with on-board expert systems are considered, with emphasis being placed on the “big three”: Vitek 2, BD Phoenix, and MicroScan. By necessity and in places, the review becomes a general review of automated system performances for the detection of specific resistance mechanisms rather than focusing solely on expert systems. Published performance evaluations of each system are drawn together and commented on critically.

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Evaluation of the Phoenix Automated Microbiology System for Detecting Extended-Spectrum β-Lactamase in Escherichia coli, Klebsiella species and Proteus mirabilis

Cited by 4 publications

References 15 publications

Development and Evaluation of Oligonucleotide Chip Based on the 16S-23S rRNA Gene Spacer Region for Detection of Pathogenic Microorganisms Associated with Sepsis

Development and Evaluation of Oligonucleotide Chip Based on the 16S-23S rRNA Gene Spacer Region for Detection of Pathogenic Microorganisms Associated with Sepsis

A Comparison of The Resistance Ratio Obtained in The Three Different Time Intervals in Escherichia coli Strains Against Ciprofloxacin, Cefotaxime and Imipenem

Expert Systems in Clinical Microbiology

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