Evaluation of the microenzyme-linked immunosorbent assay with Treponema pallidum antigen

Pope, Victoria; Hunter, Elizabeth F.; Feeley, John C.

doi:10.1128/jcm.15.4.630-634.1982

Cited by 43 publications

(35 citation statements)

References 11 publications

(5 reference statements)

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“…The enzyme-linked immunosorbent assay (ELISA) for antibody to T. pallidum has been shown to offer several advantages over the current confirmatory tests for syphilis (3,9). Two of these advantages are the stability of the reagents and the use of optical density (OD) values for a more objective interpretation of the results.…”

mentioning

confidence: 99%

Evaluation of the syphilis Bio-EnzaBead assay for detection of treponemal antibody

Burdash¹,

Hinds²,

Finnerty³

et al. 1987

J Clin Microbiol

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An enzyme-linked immunosorbent assay for Treponema pallidum antibody (Syphilis Bio-EnzaBead Kit; Organon Teknika Corp., formerly Litton Bionetics, Kensington, Md.) was compared with the standard fluorescent treponemal antibody absorption test for syphilis (indirect fluorescent-antibody confirmatory test; Zeus Scientific Inc., Raritan, N.J.). Six hundred specimens tested in the rapid plasma Reagin card test (Hynson, Westcott and Dunning, Inc., Baltimore, Md.) and microhemagglutination assay for antibodies to T. pallidum (Ames Division, Miles Laboratories, Inc., Elkhart, Ind.) were used for comparison testing. One hundred and sixty-two specimens were from either persons with syphilis or persons whose history was unknown but who showed reactive serology, whereas 438 were from persons without syphilis. The reactivity of each serum by the Bio-EnzaBead test was determined visually and from optical density readings. Excellent reproducibility of visual readings was obtained; all results were within ± 1 gradation of the expected readings in all 60 coded sera tested. Overall agreement between the Bio-EnzaBead and fluorescent treponemal antibody absorption test results was 96.3% when read visually and .95.7% when using optical density readings. Our data indicate that Bio-EnzaBead results read at 405 nm and determined by using the mean antigen optical density reading of the nonreactive control plus 0.025 as the cutoff value provide an overall sensitivity and specificity of 93.0 and 98.6%, respectively, along with the best agreement with the fluorescent treponemal antibody absorption assay. Ease of performance and objectivity also contribute toward the acceptability of this assay as an alternative confirmatory test for syphilis.

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mentioning

confidence: 99%

Evaluation of the syphilis Bio-EnzaBead assay for detection of treponemal antibody

Burdash¹,

Hinds²,

Finnerty³

et al. 1987

J Clin Microbiol

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“…The use of sonicates of Treponema pallidum (6,8,9) or the axial filaments of T. phagedenis biotype Reiter as antigens in the enzyme-linked immunosorbent assay (ELISA) test for syphilis has been described (P. H. Hardy, E. E. Nell, and A. J. O'Beirne, Abstr. Annu.…”

mentioning

confidence: 99%

Sodium desoxycholate-extracted treponemal antigen in an enzyme-linked immunosorbent assay for syphilis

Hunter¹,

Farshy²,

Liska³

et al. 1982

J Clin Microbiol

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The extraction of Treponema pallidum antigen with sodium desoxycholate, based on a previously described procedure (J. Portnoy and H. J. Magnuson, J. Immunol. 75:348-355, 1955), was used in an enzyme-linked immunosorbent assay (ELISA) test for syphilis. The antigen was prepared from T. pallidum street strain no. 14, and its overall sensitivity and specificity was compared with those of sonicated antigen preparations made with phosphate-buffered saline. The optimum serum dilution for testing and the significant absorbance reading at 490 nm were selected by examination of quantitative dilutions of 91 sera from presumably normal individuals and 92 sera from syphilitics. The time and temperature of serum and conjugate incubations were also examined. With an absorbance reading of -0.2 at the 1:80 serum dilution, 88 (95.8%) of 92 sera from syphilitics were reactive in the ELISA test with desoxycholate-extracted antigen, and 82 (89.1%) were reactive with the sonicated antigen. Only one nonsyphilitic serum was reactive with each antigen. Greater sensitivity without loss in specificity was obtained with longer serum and conjugate incubations. We concluded that an ELISA test with sodium desoxycholate-extracted antigen is more sensitive than and equally specific to an ELISA with sonicated treponemal antigen.

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“…In addition, NIHII mice were inoculated with virulent organisms. These B-and T-lymphocytedeficient mice [15] also failed to develop treponemal lesions, and they did not die earlier than the non-infected controls.…”

Section: Effect Of Inoculation Of T Pallidum Into Immunocompromised mentioning

confidence: 89%

“…The treponemes were allowed to stand for 7 days at 4°C in glass tubes [15]. Before use, the treponemes were sonicated (three times for 15 sec each in a Raytheon sonifier with a setting of 40-50 platevoltage).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the Inbred Mouse as a Model for Experimental Treponema pallidum Infection

et al. 1983

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Mice of several inbred strains were studied for responses to Treponema pallidum (Nichols). Mice were injected with viable treponemes and observed daily for the presence of lesions or signs of illness. Although none of the mice developed detectable lesions, they did develop specific anti-T. pallidum antibodies that were observed as early as 21 days after inoculation with viable T. pallidum. The antibodies were detected by an enzyme-linked immunosorbent assay and by passive haemagglutination.

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Evaluation of the microenzyme-linked immunosorbent assay with Treponema pallidum antigen

Cited by 43 publications

References 11 publications

Evaluation of the syphilis Bio-EnzaBead assay for detection of treponemal antibody

Evaluation of the syphilis Bio-EnzaBead assay for detection of treponemal antibody

Sodium desoxycholate-extracted treponemal antigen in an enzyme-linked immunosorbent assay for syphilis

Evaluation of the Inbred Mouse as a Model for Experimental Treponema pallidum Infection

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