Sodium desoxycholate-extracted treponemal antigen in an enzyme-linked immunosorbent assay for syphilis

Hunter, Elizabeth F.; Farshy, Carol E.; Liska, Sally; Cruce, D D; Crawford, J. Adam; Feeley, John C.

doi:10.1128/jcm.16.3.483-486.1982

Cited by 21 publications

(15 citation statements)

References 8 publications

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“…Nonetheless, our findings, coupled with those of Morrison-Plummer et al (16), suggest that electroelution-purified treponemal antigens are perhaps ideal candidate materials for RIA and enzyme immunoassays. Further testing of electroeluted materials should establish whether the sensitivity and specificity of these preparations are such that they can replace other treponemal tests, as suggested by Hunter et al (11).…”

Section: Resultsmentioning

confidence: 99%

Radioimmunoassays for the detection of antibodies to treponemal polypeptide antigens in serum

Baughn¹,

Musher²

1985

J Clin Microbiol

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Whatman, Inc., Clifton, N.J.), which in turn were subjected to linear gradients of 0 to 2 M NaCl in 0.01 M phosphate buffer, pH 7. Fractions eluting at approximately 0.15 M NaCl consisted almost entirely of flagella as verified by electron microscopy. These fractions were pooled, treated for 24 h with Nonidet P-40, and centrifuged at 100,000 x g for 2 h; the pellets were resuspended in 2 ml of 0.01 M phosphate buffer.

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Section: Resultsmentioning

confidence: 99%

Radioimmunoassays for the detection of antibodies to treponemal polypeptide antigens in serum

Baughn¹,

Musher²

1985

J Clin Microbiol

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“…Ten normal serum specimens were tested and found to be nonreactive (OD of <0.2) in the treponemal and RF ELISAs. An OD of 0.2 was determined to be the optimal cut-off point between positive and negative values based on previous studies with sera from patients with syphilis and patients without syphilis (12).…”

Section: Resultsmentioning

confidence: 99%

“…ELISA antigen. Treponemal antigen was prepared as previously described (12). Briefly, T. pallidum was grown by intratesticular inoculation in adult male rabbits.…”

Section: Methodsmentioning

confidence: 99%

Rheumatoid factor in syphilis

Cerný¹,

Farshy²,

Hunter³

et al. 1985

J Clin Microbiol

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Immunoglobulin M (IgM) antibodies directed against IgG antibodies (rheumatoid factor [RF]) are known to occur often in patients with syphilis and to interfere with serological tests measuring specific antibodies of the IgM class. In this study we examined the occurrence and specificity of the RF and demonstrated a simple method to detect and eliminate the RF for a specific Treponema pallidum IgM enzyme-linked immunosorbent assay. We measured the occurrence of the RF with a sensitive enzyme-linked immunosorbent assay and found that it increased with the duration of syphilitic disease: 1 of 13 primary syphilis serum specimens, 3 of 13 secondary syphilis serum specimens, and 10 of 27 latent syphilis serum specimens were reactive in this RF test. Those sera containing IgM RF were immunoprecipitated with anti-human gamma chain antibodies and 2% polyethylene glycol until the RF was removed. One serum specimen from a patient in the secondary stage of syphilis and eight serum specimens from patients with latent disease still presented the RF after immunoprecipitation. Removal of the IgG antibodies also improved the sensitivity of the treponemal IgM test, indicating competition of these antibodies for binding sites of the antigen. The enzyme-linked immunosorbent assays for detection of RF and antitreponemal IgM antibodies are performed on the same plate. Theoretically, only sera positive for both tests have to be immunoprecipitated. But our findings indicated an increase in sensitivity of the IgM enzyme-linked immunosorbent assay after removal of IgG antibodies responsible for competition at the binding sites.

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“…The first application of the ELISA to the area of syphilis serology was in 1975 (15). Since that time, several ELISA methods for syphilis have been described (16)(17)(18)(19)(20)(21)(22). In 1984, the first commercially available ELISA technique for the serodiagnosis of syphilis received Provisional Status from the CDC.…”

Section: Introductionmentioning

confidence: 99%

Review of the standard tests for syphilis and evaluation of a new commercial elisa, the syphilis bio‐enzabead test

Larsen

Hambie

Cruce

1987

Clinical Laboratory Analysis

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To date, there are eight serologic tests rable to the sensitivity of the microhemagconsidered Standard for the serodiagnosis glutination assay (MHA-TP) of 97.6% in of syphilis; however, the syphilis Bio-untreated syphilis. The specificity of the EnzaBead test is the first commercially syphilis Bio-EnzaBead when read as deavailable enzyme-linked immunosorbent scribed above is 94.7%, compared with assay (ELISA) for the confirmation of a re-the 99.3% of the MHA-TP and the 98.1% active nontreponemal test result in the se-of the fluorescent treponemal antibody-abrodiagnosis of syphilis. The Bio-EnzaBead sorption (FTA-ABS) test. Heating of the has a sensitivity of 95.2% in untreated specimens did not adversely affect the test syphilis when read at 405 nm with 80% of outcome, and it eliminated background the 1 + optical density (OD) used as the readings seen with the negative control breakpoint OD between reactive and non-beads. reactive results. This sensitivity is compa-

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Sodium desoxycholate-extracted treponemal antigen in an enzyme-linked immunosorbent assay for syphilis

Cited by 21 publications

References 8 publications

Radioimmunoassays for the detection of antibodies to treponemal polypeptide antigens in serum

Radioimmunoassays for the detection of antibodies to treponemal polypeptide antigens in serum

Rheumatoid factor in syphilis

Review of the standard tests for syphilis and evaluation of a new commercial elisa, the syphilis bio‐enzabead test

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