Evaluation of the genotoxic potential of different delivery methods of at-home bleaching gels: a single-blind, randomized clinical trial

Monteiro, Marcílio Jorge Fernandes; Lindoso, Jéssica Bruna Corrêa; Conde, Nikeila Chacon de Oliveira; Silva, Luciana Mendonça da; Loguércio, Alessandro Dourado; Pereira, Juliana Vianna

doi:10.1007/s00784-018-2659-8

Cited by 24 publications

(19 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The potential genotoxic effect of the smoking and bleaching combination was probably neutralized by the eukaryotic cells [ 16 ]. As similar results were presented in previous studies [ 11 , 27 , 28 ] which used carbamide peroxide, bleaching treatment is being demonstrated as safe for smokers as it is for nonsmokers.…”

Section: Discussionsupporting

confidence: 89%

See 1 more Smart Citation

Influence of smoking on oral cells genotoxicity after at-home bleaching using 22% carbamide peroxide: a cohort study

et al. 2021

Self Cite

View full text Add to dashboard Cite

Objective This study evaluated the influence of smoking on the oral cells genotoxicity before and after at-home bleaching using 22% carbamide peroxide (CP). Materials and methods This is a prospective observational analytics cohort study which evaluated nonsmokers (NS; n = 24) and smokers (S; n = 16) patients. At-home bleaching was performed using 22% CP gel in individual trays for 1 h per day for 14 days in both groups. Scrapped cells from marginal gums were collected before the bleaching treatment (D0- baseline ) and 1 day (D1), 15 days (D15), and 1 month (D30) after its finishing. Cells were stained with Giemsa 10%, and the micronucleus (MN) and metanuclear alterations (MA) were counted by a trained operator in 1000 cells per patient. The collections and data analysis occurred blindly. Data was analyzed by Kruskal–Wallis, Dunn, and Mann–Whitney test (α = 0.05). Results MN frequency was not influenced by smoking or bleaching. An increase of MA was observed between D0 and D30 for both groups ( p < 0.001); however, no statistical difference was found between NS and S ( p > 0.05) in the evaluation times. Conclusion Smoking associated with 22% carbamide peroxide gel for at-home bleaching does not show genotoxic potential analyzed by the MN counts. However, a significant increase of MA was found for smokers and nonsmokers. Clinical relevance Despite of the increase in MA, smoking associated with 22% CP peroxide at-home bleaching showed no important genotoxic potential (MN) for oral cells. Therefore, at-home bleaching treatment is safe for nonsmokers and smokers even with a high carbamide peroxide concentration of 22%.

show abstract

Section: Discussionsupporting

confidence: 89%

“…Most of the studies on CP genotoxicity observed inexpressive MN counts during and after bleaching (no genotoxic effect) [ 6 , 11 , 12 , 27 ]. A previous study of our group [ 15 ] evaluated MA counts during and after at-home bleaching using 22% CP.…”

Section: Discussionmentioning

confidence: 99%

Influence of smoking on oral cells genotoxicity after at-home bleaching using 22% carbamide peroxide: a cohort study

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Tooth bleaching is a conservative and little invasive esthetic procedure. Although the literature describes different bleaching techniques, at-home tooth bleaching is the most used due to excellent long-term results (Almeida, et al, 2015;Monteiro, et al, 2019). This technique generally implies the use of a bleaching agent applied to an individualized impression tray, which the patient uses in periods varying from 30 minutes to one whole night, for at least two weeks (Almeida, et al, 2015;Alonso De La Peña, et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Can tooth bleaching agents cause genotoxicity in the oral epithelium? A systematic review with meta-analysis

Menezes

Ribeiro

Takeshita

2022

RSD

View full text Add to dashboard Cite

Objective: To assess, with a systematic review (SR) with meta-analysis, the occurrence of genotoxic effects on the oral epithelium after exposure to tooth bleaching agents. Material and methods: This review was performed according to the PRISMA protocols. To identify relevant studies, a systematic search was performed in the following electronic databases: PubMed, Scopus, Embase, LILACS, and Google Scholar. The research question was "Can tooth bleaching agents cause genotoxicity in the oral epithelium?”. The treatment effects were defined as the standardized mean difference (SMD) and 95% confidence intervals (CI) were established. Results: 154 studies were selected and, after screening titles and abstracts, seven full-text manuscripts were assessed for eligibility, of which four studies were included in the meta-analysis. There were no statistically significant differences in the frequency of micronuclei before and after exposure (SMD= -0.14, 95% CI, 0.98 to 0.60, p=0.74), with a Tau2 index = 1.00; Chi2 = 70.20; p-value <0.00001; and I² of 93%, indicating high heterogeneity among the studies. Conclusion: Considering the limitations of the present SR, tooth bleaching agents do not lead to genotoxic damage in the oral epithelium but with a small effect and low level of evidence. In this way, the use of tooth bleaching agents is safe on the oral mucosa but randomized clinical trials that are more standardized in all stages are required to produce more robust evidence.

show abstract

“…Several protocols using different bleaching agents have been proposed to overcome the issues associated with CP [ 8 ]. Among the potential bleaching agents used for at-home applications is the hydrogen peroxide (HP), which represents a reliable alternative to CP as it provides a faster release and greater efficiency, thus decreasing usage times to 30 min–1 h per day [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

A Comparative Evaluation of Nanohydroxyapatite-Enriched Hydrogen Peroxide Home Bleaching System on Color, Hardness and Microstructure of Dental Enamel

et al. 2021

View full text Add to dashboard Cite

This study aimed to evaluate two hydrogen peroxide (HP)-based at-home bleaching systems in order to analyze whether nano-hydroxyapatite (nHA) addition may represent a reliable and safe solution for tooth whitening without altering dental microstructure and hardness. Human third molars (N = 15) were treated with two bleaching agents, one containing 6%HP (6HP) and the other 6% HP nHA-enriched (6HP-nHA) with average particle diameter ranging from 5–20 nm. Their effects on enamel were assessed using a spectrophotometer, Vickers microhardness (VMH) test and Scanning Electron Microscopy (SEM), comparing the treated groups with the non-treated control group (CTR). Color analysis revealed improvement in whiteness in both groups compared to CTR. VMH test results showed no differences among the groups. SEM analysis highlighted no evident changes in the enamel microstructure of tested groups compared to CTR. At high magnification, in 6HP group, a slight increase in irregularities of enamel surface morphology was observed, while 6HP-nHA group displayed removal of the aprismatic layer but preservation of the intact prismatic structure. These results suggest that the 6HP-nHA agent may be recommended to provide reliable whitening treatment, without damaging the enamel micromorphology and hardness.

show abstract

Evaluation of the genotoxic potential of different delivery methods of at-home bleaching gels: a single-blind, randomized clinical trial

Cited by 24 publications

References 44 publications

Influence of smoking on oral cells genotoxicity after at-home bleaching using 22% carbamide peroxide: a cohort study

Influence of smoking on oral cells genotoxicity after at-home bleaching using 22% carbamide peroxide: a cohort study

Can tooth bleaching agents cause genotoxicity in the oral epithelium? A systematic review with meta-analysis

A Comparative Evaluation of Nanohydroxyapatite-Enriched Hydrogen Peroxide Home Bleaching System on Color, Hardness and Microstructure of Dental Enamel

Contact Info

Product

Resources

About