Evaluation of the functional roles of fungal endophytes of Phragmites australis from high saline and low saline habitats

Soares, Marcos Antônio; Li, Haiyan; Kowalski, Kurt P.; Bergen, Marshall S.; Torres, Mónica S.; White, James F.

doi:10.1007/s10530-016-1160-z

Cited by 50 publications

(42 citation statements)

References 110 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…oxysporum colonizes only plants from places that are free from contamination with heavy metals [29,45,73,80,81]. Our results confirm this observation, since isolates of F .…”

Section: Discussionsupporting

confidence: 83%

Endophytic fungal communities of Polygonum acuminatum and Aeschynomene fluminensis are influenced by soil mercury contamination

et al. 2017

Self Cite

View full text Add to dashboard Cite

The endophytic fungal communities of Polygonum acuminatum and Aeschynomene fluminensis were examined with respect to soil mercury (Hg) contamination. Plants were collected in places with and without Hg +2 for isolation and identification of their endophytic root fungi. We evaluated frequency of colonization, number of isolates and richness, indices of diversity and similarity, functional traits (hydrolytic enzymes, siderophores, indoleacetic acid, antibiosis and metal tolerance) and growth promotion of Aeschynomene fluminensis inoculated with endophytic fungi on soil with mercury. The frequency of colonization, structure and community function, as well as the abundant distribution of taxa of endophytic fungi were influenced by mercury contamination, with higher endophytic fungi in hosts in soil with mercury. The presence or absence of mercury in the soil changes the profile of the functional characteristics of the endophytic fungal community. On the other hand, tolerance of lineages to multiple metals is not associated with contamination. A. fluminensis depends on its endophytic fungi, since plants free of endophytic fungi grew less than expected due to mercury toxicity. In contrast plants containing certain endophytic fungi showed good growth in soil containing mercury, even exceeding growth of plants cultivated in soil without mercury. The data obtained confirm the hypothesis that soil contamination by mercury alters community structure of root endophytic fungi in terms of composition, abundance and species richness. The inoculation of A. fluminensis with certain strains of stress tolerant endophytic fungi contribute to colonization and establishment of the host and may be used in processes that aim to improve phytoremediation of soils with toxic concentrations of mercury.

show abstract

“…oxysporum colonizes only plants from places that are free from contamination with heavy metals [29,45,73,80,81]. Our results confirm this observation, since isolates of F .…”

Section: Discussionsupporting

confidence: 83%

Endophytic fungal communities of Polygonum acuminatum and Aeschynomene fluminensis are influenced by soil mercury contamination

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…, Soares et al. , Shearin et al. ), characterized and compared soil oomycete communities (Nelson and Karp , Crocker et al.…”

Section: Introductionmentioning

confidence: 99%

“…As a species considered invasive in most of North America, Phragmites has been well studied, including some exploration of select microbial groups. Previous studies have examined leaf, root, and seed fungal endophytes of nonnative Phragmites (Clay et al 2016, Soares et al 2016, Shearin et al 2018, characterized and compared soil oomycete communities (Nelson and Karp 2013, Crocker et al 2015, Cerri et al 2017, and compared soil bacterial (Bowen et al 2017) and archaeal (Yarwood et al 2016) communities between Phragmites lineages. However, the functional significance of Phragmites-associated microbiomes remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Root endophytes and invasiveness: no difference between native and non‐native Phragmites in the Great Lakes Region

et al. 2018

Self Cite

View full text Add to dashboard Cite

Microbial interactions could play an important role in plant invasions. If invasive plants associate with relatively more mutualists or fewer pathogens than their native counterparts, then microbial communities could foster plant invasiveness. Studies examining the effects of microbes on invasive plants commonly focus on a single microbial group (e.g., bacteria) or measure only plant response to microbes, not documenting the specific taxa associating with invaders. We surveyed root microbial communities associated with co‐occurring native and non‐native lineages of Phragmites australis, across Michigan, USA. Our aim was to determine whether (1) plant lineage was a stronger predictor of root microbial community composition than environmental variables and (2) the non‐native lineage associated with more mutualistic and/or fewer pathogenic microbes than the native lineage. We used microscopy and culture‐independent molecular methods to examine fungal colonization rate and community composition in three major microbial groups (bacteria, fungi, and oomycetes) within roots. We also used microbial functional databases to assess putative functions of the observed microbial taxa. While fungal colonization of roots was significantly higher in non‐native Phragmites than the native lineage, we found no differences in root microbial community composition or potential function between the two Phragmites lineages. Community composition did differ significantly by site, with soil saturation playing a significant role in structuring communities in all three microbial groups. The relative abundance of some specific bacterial taxa did differ between Phragmites lineages at the phylum and genus level (e.g., Proteobacteria, Firmicutes). Purported function of root fungi and respiratory mode of root bacteria also did not differ between native and non‐native Phragmites. We found no evidence that native and non‐native Phragmites harbored distinct root microbial communities; nor did those communities differ functionally. Therefore, if the trends revealed at our sites are widespread, it is unlikely that total root microbial communities are driving invasion by non‐native Phragmites plants.

show abstract

“…In natural systems, in particular, salinity stress is predicted to increase in the future with sea-level rise and an increase in hurricanes and storms that cause saltwater intrusion into freshwater and brackish areas. Microbial symbionts are well known to promote salinity tolerance of the plant host [7][8][9][10]. On the other hand, weakened plant condition due to salinity stress may allow pathogens to express more virulent lifesyles [11].…”

Section: Introductionmentioning

confidence: 99%

“…F. solani is known for its pathogenicity in many crop systems including peas, beans, potatoes and curbutis [12,13]. We use Oryza sativa (rice) as a model plant from wetland environments [7] to test the effect of salinity on symbiont lifestyle switching.…”

Section: Introductionmentioning

confidence: 99%

Does salinity affect lifestyle switching in the plant pathogen Fusarium solani?

Eydoux

Farrer

2020

Access Microbiology

View full text Add to dashboard Cite

Symbiotic microbes that live within plant hosts can exhibit a range in function from mutualistic to pathogenic, but the reason for this lifestyle switching remains largely unknown. Here we tested whether environmental stress, specifically salinity, is a factor that can trigger lifestyle switching in a fungus mainly known as a pathogen, Fusarium solani. F. solani was isolated from roots of Phragmites australis (common reed) in saline coastal marshes of Louisiana, USA, and we used Oryza sativa (rice) as a model organism from wetland environments to test the symbiont lifestyle. We plated rice seeds on control plates or plates with F. solani at three levels of salinity (0, 8 and 16 p.p.t.), then assessed germination and seedling growth after 20 days. Salinity strongly reduced percentage germination, slowed the timing of germination and reduced growth of rice. F. solani slowed germination, and it also caused a minor increase in root growth at medium salinity and a minor decrease in root growth at high salinity. Overall, despite being a common pathogen in other crop species (peas, beans, potatoes and many types of cucurbits), we found little evidence that F. solani has a strong pathogenic lifestyle in rice and we found weak evidence that pathogenicity may increase slightly with elevated salinity. These results have implications for both crops and native plant health in the future as soil salinization increases worldwide.

show abstract

Evaluation of the functional roles of fungal endophytes of Phragmites australis from high saline and low saline habitats

Cited by 50 publications

References 110 publications

Endophytic fungal communities of Polygonum acuminatum and Aeschynomene fluminensis are influenced by soil mercury contamination

Endophytic fungal communities of Polygonum acuminatum and Aeschynomene fluminensis are influenced by soil mercury contamination

Root endophytes and invasiveness: no difference between native and non‐native Phragmites in the Great Lakes Region

Does salinity affect lifestyle switching in the plant pathogen Fusarium solani?

Contact Info

Product

Resources

About