Evaluation of the cytotoxic and cytostatic activities of alkaloid extracts from different parts of Peganum harmala L. (Zygophyllaceae)

Bournine, Lamine; Bensalem, Sihem; Fatmi, Sofiane; Bedjou, Fatiha; Mathieu, Véronique; Iguer-Ouada, Mokrane; Kiss, Róbert; Duez, Pierre

doi:10.1016/j.eujim.2016.10.002

Cited by 36 publications

(22 citation statements)

References 27 publications

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“…Harmine and its analogs, such as harmaline and harmane ( Figure 1 ), belonging to the β-carboline alkaloids, were isolated from Peganum harmala and Eurycoma longifolia [ 6 ]. These compounds have been garnering increasing interest due to numerous activities [ 7 ], including antitumor [ 8 , 9 ], antiviral [ 10 ], analgesic [ 11 ] and anti-inflammatory activities [ 12 ]. Although several reports have been published on the insecticidal activities, growth inhibitory activities and/or oviposition deterrence of solvent extracts and β-carboline alkaloids of P. harmala against Tribolium castaneum [ 13 ], Rhyzopertha dominica [ 14 ], Plutella xylostella [ 15 ], Spodoptera littoralis [ 16 , 17 ], Schistocerca gregaria [ 18 ] and Bactrocera oleae [ 19 ], little is known about the bioactivity of harmine against Spodoptera litura .…”

Section: Introductionmentioning

confidence: 99%

Design, Synthesis and Bioactivity Evaluation of Novel β-carboline 1,3,4-oxadiazole Derivatives

Zhang

Jiang

et al. 2017

Molecules

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A series of novel β-carboline 1,3,4-oxadiazole derivatives were designed and synthesized, and the in vitro cytotoxic activity against Sf9 cells and growth inhibitory activity against Spodoptera litura were evaluated. Bioassay results showed that most of these compounds exhibited excellent in vitro cytotoxic activity. Especially, compound 37 displayed the best efficacy in vitro (IC50 = 3.93 μM), and was five-fold more potent than camptothecin (CPT) (IC50 = 18.95 μM). Moreover, compounds 5 and 37 could induce cell apoptosis and cell cycle arrest and stimulate Sf-caspase-1 activation in Sf9 cells. In vivo bioassay also demonstrated that compounds 5 and 37 could significantly inhibit larvae growth of S. litura with decreasing the weight of larvae and pupae. Based on these bioassay results, compounds 5 and 37 emerged as lead compounds for the development of potential insect growth inhibitions.

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Section: Introductionmentioning

confidence: 99%

Design, Synthesis and Bioactivity Evaluation of Novel β-carboline 1,3,4-oxadiazole Derivatives

Zhang

Jiang

et al. 2017

Molecules

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“…The MDA-MB-231 cell line in this study was chosen, because it has been proven as a good in vitro model system to investigate the cytotoxicity and anticancer potential of various plant extracts against breast cancer (Alshatwi, 2011;Dilshad et al, 2012, Ghafari et al, 2017Nasr et al, 2018). Traditionally medicinal plants have been proven to play an important role in the treatment of various cancer diseases (Bournine et al, 2017). Numerous researches have been shown beneficial effects of plant extracts against various cancer cell lines (Pan and Ho, 2008;Farshori et al, 2013.…”

Section: Discussionmentioning

confidence: 99%

“…(Dragland et al, 2003). Traditional medicinal plants have been proven to play an important role in the treatment of various cancer diseases, since ancient (Bournine et al, 2017). Therapeutically significant bioactive compounds, such as Taxol, Vinblastine, Vinflunine, Vinorelbine, Camptothecin, Vincristine and Vindesine have been explored from plants and are established to treat the various cancer diseases (Greenwell and Rahman, 2015).…”

Section: Research Articlementioning

confidence: 99%

Cytotoxic Potential of Petroleum ether, Ethyl Acetate, Chloroform, and Ethanol Extracts of Lavandula Coronopifolia Against Human Breast Carcinoma Cell line (MDA-MB-321)

Al-Sheddi

2019

Asian Pac J Cancer Prev

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Background: Breast cancer is the most common cause of deaths in women. The search for traditionally used medicinal plants which can serve as non-toxic and affordable anticancer drugs is the need of the hour. This study aimed to investigate the anticancer potential of extracts of L. coronopifolia against human breast carcinoma cell line (MDA-MB-321). Methods: The MDA-MB-231 cells were plated in 96 well plates and exposed to 10-1,000 µg/ml of L. coronopifolia for 24 h. The cytotoxic response of different extracts was measured by MTT assay, neutral red uptake (NRU) assay and cellular morphological alterations under the microscope. Results: A concentration-dependent decrease in the cell viability of MDA-MB-231 cells was observed after the exposure of petroleum ether, ethyl acetate, chloroform, and ethanol extracts of L. coronopifolia. The cell viability was found to be 82%, 89% and 98% at 1000, 500 and 250 µg/ml, respectively in petroleum ether, 37%, 75% and 88% at 1,000, 500 and 250 µg/ml, respectively in ethyl acetate extract, 30%, 35% and 64% at 1,000, 500 and 250 µg/ml, respectively in chloroform extract and 44%, 65% and 82% at 1000, 500 and 250 µg/ml, respectively in ethanolic extract of L. coronopifolia exposed MDA-MB-231 cells. The results also exhibited morphological alterations in MDA-MB-231 cells exposed to various extracts. The cells treated with 250-1000 µg/ml lost their original morphology and cell linkage as compared to control cells. Conclusion: These preliminary results suggest the promising anticancer potential of petroleum ether, ethyl acetate, chloroform, and ethanol extracts of L. coronopifolia against MDA-MB-321 cells. Further studies are required to know the mechanism(s) involved in the cell death.

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“…Aiming to develop essential chemotherapeutic drugs to control various types of cancer, one of the primary tests is the tetrazolium reduction method. This test can determine the minimum inhibitory concentration required to obtain 50% of viable or unviable cells by the cytotoxicity promoted by the tested agent, the time to drug exposure and the efficacy of drug administration [2,3].…”

Section: Introductionmentioning

confidence: 99%

In vitro methodological standardization of MTT colorimetric assay for evaluation of formazan activity and drug dosage

Nepomuceno

Carvalho

Soares

et al. 2020

Preprint

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Background Aiming to develop essential chemotherapeutic drugs to control various types of cancer, one of the primary tests is the tetrazolium reduction method. The present study aims to standardize the electromagnetic spectrum that best discriminates the absorbance and to evaluate the drug dosage of single and daily doses during the cell viability and toxicity analysis by the tetrazolium reduction method, using the ethanol extract of pequi peel in canine osteosarcoma cells. Results The wavelength of 532 nm showed the best results. We found that as the treatment time increased, the formazan conversion reduced. After 72 hours of treatment, we observed clear discrimination of dose-dependent data, with up to five discriminants within 72 hours with a change in absorbance from 0.554 to 0.064 A. The wavelength of 570 nm it was not ideal since we could not discern the difference in the spectral reflectance of the treatments and, therefore, show no statistical difference among treatments. We found no statistical difference for the coefficient of variation at wavelengths of 532 and 570nm, which were 12.77% and 8.80% respectively. Conclusions The wavelength of 532nm best discriminated the absorbance, as it presented better ability to congregate the treatment groups, greater variation between the discriminant and lower coefficient of variation, during the colorimetric test to evaluate the cellular metabolism. Moreover, the ethanol extract of pequi peel in canine osteosarcoma cells showed a statistically equal effect of a single dose administration to the dose reapplied every 24 hours.

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Evaluation of the cytotoxic and cytostatic activities of alkaloid extracts from different parts of Peganum harmala L. (Zygophyllaceae)

Cited by 36 publications

References 27 publications

Design, Synthesis and Bioactivity Evaluation of Novel β-carboline 1,3,4-oxadiazole Derivatives

Design, Synthesis and Bioactivity Evaluation of Novel β-carboline 1,3,4-oxadiazole Derivatives

Cytotoxic Potential of Petroleum ether, Ethyl Acetate, Chloroform, and Ethanol Extracts of Lavandula Coronopifolia Against Human Breast Carcinoma Cell line (MDA-MB-321)

In vitro methodological standardization of MTT colorimetric assay for evaluation of formazan activity and drug dosage

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