Cytotoxic Potential of Petroleum ether, Ethyl Acetate, Chloroform, and Ethanol Extracts of Lavandula Coronopifolia Against Human Breast Carcinoma Cell line (MDA-MB-321)

Abstract

Background: Breast cancer is the most common cause of deaths in women. The search for traditionally used medicinal plants which can serve as non-toxic and affordable anticancer drugs is the need of the hour. This study aimed to investigate the anticancer potential of extracts of L. coronopifolia against human breast carcinoma cell line (MDA-MB-321). Methods: The MDA-MB-231 cells were plated in 96 well plates and exposed to 10-1,000 µg/ml of L. coronopifolia for 24 h. The cytotoxic response of different extracts was measured by MTT assay, neutral red uptake (NRU) assay and cellular morphological alterations under the microscope. Results: A concentration-dependent decrease in the cell viability of MDA-MB-231 cells was observed after the exposure of petroleum ether, ethyl acetate, chloroform, and ethanol extracts of L. coronopifolia. The cell viability was found to be 82%, 89% and 98% at 1000, 500 and 250 µg/ml, respectively in petroleum ether, 37%, 75% and 88% at 1,000, 500 and 250 µg/ml, respectively in ethyl acetate extract, 30%, 35% and 64% at 1,000, 500 and 250 µg/ml, respectively in chloroform extract and 44%, 65% and 82% at 1000, 500 and 250 µg/ml, respectively in ethanolic extract of L. coronopifolia exposed MDA-MB-231 cells. The results also exhibited morphological alterations in MDA-MB-231 cells exposed to various extracts. The cells treated with 250-1000 µg/ml lost their original morphology and cell linkage as compared to control cells. Conclusion: These preliminary results suggest the promising anticancer potential of petroleum ether, ethyl acetate, chloroform, and ethanol extracts of L. coronopifolia against MDA-MB-321 cells. Further studies are required to know the mechanism(s) involved in the cell death.

Cytotoxic effects of Lavandula angustifolia seed extracts on the viability of Huh-7 and Chang liver cells

“…Natural plant species have been used not only as sources of food, but also as remedies for many diseases in the ancient era. Different reports suggested that there are unidentified properties in different parts of plants that can be used in the treatment of certain diseases [ 6 ]. The current investigation intended to reveal the potential anticancer and anti-proliferative activities of lavender plant extracts in liver cancer cell line in an in vitro model.…”

In Vitro Assessment of Antiplasmodial and Antitrypanosomal Activities of Chloroform, Ethyl Acetate and Ethanol Leaf Extracts of Oedera genistifolia

“…Widiyastuti et al [39] reported that the ethanolic extracts (flowers, stems and leaves) of Stachytarpheta jamaicensis (L.) Vahl had no toxic effect on HeLa cells. On the contrary, Al-Sheddi et al [40] reported the cytotoxic effect of ethyl acetate, chloroform and ethanol extracts of Lavandula coronopifolia against MDA-MB-321 cells and in the same vein, the crude leaves ethanol extract of Lasiosiphon eriocephalus exhibited a high toxicity effect against HeLa cells [41]. In vitro cytotoxicity studies revealed that ethanol extracts of leaves of Tamilnadia uliginosa exhibited toxic effects on HeLa cells [42].…”