Evaluation of the COM.TEC cell separator in predicting the yield of harvested CD34+ cells

Movassaghi, Kamran; Jaques, Gabrielle; Schmitt-Thomssen, A.; Fischer, Eike G.; Paulus, Margit; Heuft, H G; Schwella, Nimord

doi:10.1111/j.1537-2995.2007.01196.x

Cited by 4 publications

(8 citation statements)

References 44 publications

(94 reference statements)

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“…The autoMNC program is an established program that has been shown to result in higher CE and better prediction of the CD34+ yield. [ 17 18 ]…”

Section: Methodsmentioning

confidence: 99%

“…Collection efficiency, a percentage measure of the cell separator's ability to extract maximum number of CD34+ cells from the cells available in the donor's blood was calculated as follows[ 15 16 17 18 19 ]:…”

Section: Methodsmentioning

confidence: 99%

“…In addition, an alternate formula, which factored in the postprocedural peripheral blood CD34+ cell count was used to calculate CE in 48 autologous cases. [ 18 19 20 ]…”

Section: Methodsmentioning

confidence: 99%

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Autologous peripheral blood stem cell harvest: Collection efficiency and factors affecting it

Tiwari

Pandey

Subbaraman

et al. 2016

Asian J Transfus Sci

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Background:Harvest of hematopoietic progenitor cells via leukapheresis is being used increasingly for transplants in India. Adequate yield of cells per kilogram body weight of recipient is required for successful engraftment. Collection efficiency (CE) is an objective quality parameter used to assess the quality of leukapheresis program. In this study, we calculated the CE of the ComTec cell separator (Fresenius Kabi, Germany) using two different formulae (CE1 and CE2) and analyzed various patient and procedural factors, which may affect it.Materials and Methods:One hundred and one consecutive procedures in 77 autologous donors carried out over 3 years period were retrospectively reviewed. Various characteristics like gender, age, weight, disease status, hematocrit, preprocedure total leukocyte count, preprocedure CD34 positive (CD34+) cells count, preprocedure absolute CD34+ cell count and processed apheresis volume effect on CE were compared. CE for each procedure was calculated using two different formulae, and results were compared using statistical correlation and regression analysis.Results:The mean CE1 and CE2 was 41.2 and 49.1, respectively. CE2 appeared to be more accurate indicator of overall CE as it considered the impact of continued mobilization of stem cells during apheresis procedure, itself. Of all the factors affecting CE, preprocedure absolute CD34+ was the only independent factor affecting CE.Conclusion:The only factor affecting CE was preprocedure absolute CD34+ cells. Though the mean CE2 was higher than CE1, it was not statistically significant.

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“…The autoMNC program is an established program that has been shown to result in higher CE and better prediction of the CD34+ yield. [ 17 18 ]…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Autologous peripheral blood stem cell harvest: Collection efficiency and factors affecting it

Tiwari

Pandey

Subbaraman

et al. 2016

Asian J Transfus Sci

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“…The auto mononuclear cell (MNC) collection program of the COM.TEC cell separator (Fresenius HemoCare GmbH, Bad Homburg, Germany) was recently introduced for the collection of PBPCs in patients and allogeneic donors. There have been several reports regarding collection variables and contents of the harvest with the COM.TEC cell separator 4‐6 and its comparative performance with other cell separators 7 . However, there have been no reports on the performance and clinical feasibility of the instrument for the collection of PBPC in pediatric groups.…”

mentioning

confidence: 99%

Evaluation of a new cell separator for collection of peripheral blood CD34+ progenitor cells in pediatric patients

Kim¹,

Choung²,

Kim³

et al. 2010

Transfusion

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PBPC collections by the COM.TEC cell separator had advantages of high processing rate, low product volume, and low contamination by PLTs of product. Low PLT loss was observed in pediatric patients who need to collect autologous PBPCs. However, applying CD34+ cell yield prediction was not practical for prospective scheduling of the next collection. More specified data need to be accumulated for more accurate prediction of CD34+ cell yield in pediatric patients.

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“…The amount of CD34 1 cells mobilized greatly varies patient wise, depending on: diagnoses, previous chemotherapy and, probably, other poorly known factors [11,17,18]. The yield of CD34 1 cells per apheresis depends on mobilizing agents and other factors, the most important are: the number of previous chemotherapy cycles, the time elapsed between the last chemotherapy and the apheresis procedure, and the amount of PBPCs at the beginning of the apheresis [19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Large‐volume‐apheresis facilitates autologous transplantation of hematopoietic progenitors in poor mobilizer patients

Majado

Minguela

González-García

et al. 2009

J of Clinical Apheresis

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Given that pre-apheresis CD34(+) cell count (PA-CD34) predicts the apheresis' yield, a minimum of 5 to 20 PA-CD34/microl is required in many institutions to initiate cell collection. The aim of this study was to clarify whether large-volume-apheresis (LVA) could facilitate progenitor cell transplantation in patients with low PA-CD34. Apheresis was initiated in 226 patients, disregarding PA-CD34, at days: +5 in G-CSF, +10 in cyclophosphamide+G-CSF, and +15 to +20 in other chemotherapy+G-CSF mobilization, when leucocytes >2.5 x 10(9)/L. Four times the blood volume was processed. Patients were grouped according to their PA-CD34: >or=10/microl (group-A, n = 143); <10/microl but >or=5/microl (group-B, n = 40) and <5/microl (group-C, n = 43). No differences were found in diagnoses, gender, age, previous treatments or mobilization regimen between groups. Enough CD34(+) cells (>1.9 x 10(6)/kg) were obtained in 31 patients (72%) from group-C, although in this group two mobilizations were needed in 20 patients (46.5%), compared to 5 (3.5%) and 1 (2.5%) in groups A and B, respectively (P < 0.01). Evenly three apheresis or more were required in 28 patients (65.1%) from group-C, compared to 8 (5.6%) and 6 (15.0%) in groups A and B, respectively (P < 0.01). In conclusion LVA can facilitate autologous transplantation in poor-mobilizer-patients, low PA-CD34 should not be an inflexible exclusion factor.

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Evaluation of the COM.TEC cell separator in predicting the yield of harvested CD34+ cells

Cited by 4 publications

References 44 publications

Autologous peripheral blood stem cell harvest: Collection efficiency and factors affecting it

Autologous peripheral blood stem cell harvest: Collection efficiency and factors affecting it

Evaluation of a new cell separator for collection of peripheral blood CD34+ progenitor cells in pediatric patients

Large‐volume‐apheresis facilitates autologous transplantation of hematopoietic progenitors in poor mobilizer patients

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