Evaluation of a new cell separator for collection of peripheral blood CD34+ progenitor cells in pediatric patients

Abstract

PBPC collections by the COM.TEC cell separator had advantages of high processing rate, low product volume, and low contamination by PLTs of product. Low PLT loss was observed in pediatric patients who need to collect autologous PBPCs. However, applying CD34+ cell yield prediction was not practical for prospective scheduling of the next collection. More specified data need to be accumulated for more accurate prediction of CD34+ cell yield in pediatric patients.

Autotransplantation of circulating endothelial progenitor cells protects against lipopolysaccharide-induced acute lung injury in rabbit

“…avoiding immunological rejection and allergic response, simple to manage and easily acceptable to patients. In addition, collecting blood mononuclear cells by blood cell separator is able to eliminate the damage and loss of other blood components during the process of isolating EPC from peripheral blood [18]. Therefore, we decided to use EPCs autotransplantation, a therapeutic method with clinical practical value, in the present study.…”

Efficacy of deferred dosing of granulocyte colony-stimulating factor in autologous hematopoietic transplantation for multiple myeloma

“…9, 10 Standard of care prior to transplantation now uses agents with less myelosuppressive activity than oral melphalan, the agent most widely used in the past. Moreover, CD-34 + cell collection techniques have improved 11 and alternative agents such as plerixafor are now available to assist mobilization where G-CSF/chemotherapy alone has failed. 12 As a consequence, the median number of infused CD-34 + cells/kg has significantly increased over the past decade at our own center (3.79 (2.61–9.42) vs 4.49 (2.49–10.2, P =0.021).…”

Peripheral blood stem cell collection in low‐weight children: retrospective comparison of two apheresis devices

“…Pediatric apheresis is challenging, because of extracorporeal volume, vascular access, metabolic and hematologic issues, and psychological impact. In low‐weight children, these issues are greatly magnified and depend on the device used . The choice of the collection system is of major importance for pediatric apheresis.…”

“…The choice of the collection system is of major importance for pediatric apheresis. The COBE Spectra MNC apheresis system (CaridianBCT, Lakewood, CO) is one of the most commonly used apheresis machines and until now was one of the best‐performing apheresis systems for children 5‐8. Since 2010, a novel apheresis system has been developed: the Optia v.7.0 (TerumoBCT, Lakewood, CO) 9‐11.…”

“…In low-weight children, these issues are greatly magnified and depend on the device used. 5,6 The choice of the collection system is of major importance for pediatric apheresis. The COBE Spectra MNC apheresis system (CaridianBCT, Lakewood, CO) is one of the most commonly used apheresis machines and until now was one of the best-performing apheresis systems for children.…”