Autotransplantation of circulating endothelial progenitor cells protects against lipopolysaccharide-induced acute lung injury in rabbit

Gao, Xiaofang; Chen, Weizhen; Liang, Zhixin; Chen, Liangan

doi:10.1016/j.intimp.2011.05.019

Cited by 26 publications

(21 citation statements)

References 31 publications

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“…Gao et al have demonstrated in a LPS-induced ARDS rabbit model that systemic application of progenitor cells inhibited the expression of adhesion molecules and the synthesis of pro-inflammatory cytokines (TNF-α or IL-1b) while significantly increasing the synthesis of anti-inflammatory cytokines (IL-10) [25]. In our study, we too observed a significantly reduced expression of adhesion molecules following administration of BMDPC.…”

Section: Discussionsupporting

confidence: 56%

“…In our study, we too observed a significantly reduced expression of adhesion molecules following administration of BMDPC. Interestingly, Gao et al were also able to show a significant reduction of the inflammation-induced apoptosis of endothelial and epithelial cells by the application of progenitor cells [25]. Based on these observations, it seems that BMDPC not only stimulate vessel regeneration and repair but also inhibit local inflammation [26].…”

Section: Discussionmentioning

confidence: 99%

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Bone marrow-derived progenitor cells attenuate inflammation in lipopolysaccharide-induced acute respiratory distress syndrome

et al. 2014

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BackgroundAcute respiratory distress syndrome (ARDS) is the most common cause of respiratory failure among critically ill patients. Novel treatment strategies are required to address this common clinical problem. The application of exogenous adult stem cells was associated with a beneficial outcome in various pre-clinical models of ARDS. In the present study we evaluated the functional capacity and homing ability of bone marrow-derived progenitor cells (BMDPC) in vitro and investigated their potential as a treatment strategy in lipopolysaccharide (LPS)-induced ARDS.ResultsEvaluation of the BMDPC showed functional capacity to form endothelial outgrowth cell colonies, which stained positive for CD133 and CD31. Furthermore, DiI-stained BMDPC were demonstrated to home to injured lung tissue. Rats treated with BMDPC showed significantly reduced histopathological changes, a reduced expression of ICAM-1 and VCAM-1 by the lung tissue, an inhibition of proinflammatory cytokine synthesis, a reduced weight loss and a reduced mortality (p < 0.03) compared to rats treated with LPS alone.ConclusionsThese findings suggest that the application of exogenous BMDPC can attenuate inflammation in LPS-induced ARDS and thereby reduce the severity of septic organ damage. Cell therapy strategies using adult stem cells might therefore become a novel and alternative option in ARDS therapy.

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Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Bone marrow-derived progenitor cells attenuate inflammation in lipopolysaccharide-induced acute respiratory distress syndrome

et al. 2014

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“…In previous studies, EPC transplantation has been demonstrated to be a useful therapeutic method with clinical practical value for treating ALI/ARDS [22,23]. In this study, we confirmed that EPCs could repair lung damage induced by LPS.…”

Section: Discussionsupporting

confidence: 83%

Protective Effects of Bone Marrow-Derived Endothelial Progenitor Cells and <b><i>Houttuynia Cordata</i></b> in Lipopolysaccharide-Induced Acute Lung Injury in Rats

Cai

Zhou

Liu

et al. 2013

Cell Physiol Biochem

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Background/Aims: Acute lung injury/acute respiratory distress syndrome (ALI/ARDS) is a serious health problem, and an effective treatment is needed for use in the clinical setting. Methods: In this study, we first constructed ALI models in Adult Sprague-Dawley rats. We then used an herbal medicine, Houttuynia cordata (HC), to enhance the effect of endothelial progenitor cells (EPCs) on ALI. Results: (1) HC improved the therapeutic effects of EPCs on lipopolysachharide-induced ALI in the rat model; (2) HC down-regulated the anti-inflammatory response by suppressing inflammatory cytokines; (3) the combination of EPC and HC reduced expression of iNOS and ET-1 and subsequently prevented lung injury. Conclusion: Combined EPC and HC therapy was more effective than either therapy alone. EPC and HC could be used in the clinical treatment of ALI.

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“…We used a lentiviral vector to mediate BMP2 gene transfer to [31]. Consistent with the effectiveness of EPCs shown in other studies [4][5][6][7][8], BMP2-transduced EPCs minimized LPS-induced ALI in rats in terms of pathological changes, arterial oxygen content, wet-to-dry lung-weight ratio, BALF protein content, and expression of the inflammatory cytokines TNF-α and IL-10. The endothelial-repair effect of BMP2-transduced EPCs was particularly noticeable in relation to wet-to-dry lung-weight ratio and BALF protein content, indicating improved microvascular permeability and barrier integrity.…”

Section: Discussionmentioning

confidence: 64%

“…Endothelial progenitor cells (EPCs) were first discovered by Asahara et al in 1997 [3], and their potential regenerative and immunomodulatory capabilities in oleic acidor lipopolysaccharide (LPS)-induced ALI have since been extensively documented [4][5][6][7][8]. Transplanted EPCs can differentiate into endothelial cells, preserve the function and integrity of the alveolar-capillary barrier, and adjust cytokine secretion to modulate the immune response [9,10].…”

Section: Introductionmentioning

confidence: 99%

Intravenous Transplantation of BMP2-Transduced Endothelial Progenitor Cells Attenuates Lipopolysaccharide-Induced Acute Lung Injury in Rats

Yin¹,

Liang²,

Yen³

et al. 2015

Cell Physiol Biochem

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Background/Aims:Acute lung injury (ALI) and its aggressive stage, acute respiratory distress syndrome (ARDS), are characterized by diffuse damage and increased permeability of the endothelial barrier, leading to alveolar infiltrates and interstitial edema. Enhancing endothelial integrity represents a novel therapeutic strategy for ALI/ARDS. Endothelial progenitor cells (EPCs) have been reported to participate in endothelial repair of ALI and also serve as a tool for gene therapy. Further, bone morphogenetic protein 2 (BMP2) is an essential signaling molecule that regulates the fate of different cell types. The aim of our study is to explore whether bone marrow-derived EPCs transduced with lentiviral-mediated BMP2 gene might benefit lipopolysaccharide (LPS)-induced ALI in a rat model. Methods: Rats were divided randomly into five groups. The sham group was given normal saline via the trachea and right jugular vein. The other four groups underwent intratracheal-LPS-induced ALI followed after 30 min by treatment with either normal saline, EPCs, EPCs transduced with empty lentiviral vector (EPCs-null), or EPCs transduced with BMP2 (EPCs-BMP2) via the right jugular vein. Results: We found that the lung injury score, oxygenation, and inflammatory response were significantly ameliorated in the three EPC-treated groups (EPCs, EPCs-null, and EPCs-BMP2). In addition, EPCs-BMP2 further improved endothelium repair and capillary permeability, causing markedly reduced wet-to-dry lung-weight ratio and BALF protein content, and increased levels of BMP2 protein, BMP2 mRNA, and eNOS protein in lung tissues. Conclusion: Transplantation of BMP2-transduced EPCs effectively attenuates edema and protein exudation compared with EPCs alone in LPS-induced ALI via enhanced expression of BMP2 and eNOS.

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Autotransplantation of circulating endothelial progenitor cells protects against lipopolysaccharide-induced acute lung injury in rabbit

Cited by 26 publications

References 31 publications

Bone marrow-derived progenitor cells attenuate inflammation in lipopolysaccharide-induced acute respiratory distress syndrome

Bone marrow-derived progenitor cells attenuate inflammation in lipopolysaccharide-induced acute respiratory distress syndrome

Protective Effects of Bone Marrow-Derived Endothelial Progenitor Cells and <b><i>Houttuynia Cordata</i></b> in Lipopolysaccharide-Induced Acute Lung Injury in Rats

Intravenous Transplantation of BMP2-Transduced Endothelial Progenitor Cells Attenuates Lipopolysaccharide-Induced Acute Lung Injury in Rats

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