Evaluation of the antioxidant activity of Ruellia tuberosa

Chen, Fu An; Wu, An‐Bang; Shieh, Pochuen; Kuo, Daih Huang; Hsieh, Chi Ying

doi:10.1016/j.foodchem.2004.09.046

Cited by 77 publications

(49 citation statements)

References 17 publications

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“…However, in cell experiments, RTL was extracted using different solvents and the resulting extract was found to have caused cancer cell toxicity (Lin et al., 2006). Some studies obtained different layers of extraction using different solvents to extract RTL, which was found to have free radicals (DPPH, hydrogen peroxide, and ROO¯) scavenging activity, and one of the best effects was obtained with extraction with ethyl acetate (Chen, Wu, Shieh, Kuo, & Hsieh, 2006; Phakeovilay et al., 2013). However, currently, the optimal concentration of RTL extract to be used has not been determined.…”

Section: Discussionmentioning

confidence: 99%

“…We demonstrate that various concentrations of both RTLW and RTLE enhanced glucose uptake ability in TNF‐α‐induced insulin‐resistant C2C12 cells model. It is presumed that the effect of RTL on improving the IR of C2C12 may be due to RTL contains abundant phytochemicals that possess antioxidative ability (Chen et al., 2006; Phakeovilay et al., 2013), anti‐inflammatory activity (Alam et al., 2009), etc. Our data reveal that RTL extracts may improve IR in animal or cell experiments.…”

Section: Discussionmentioning

confidence: 99%

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Ameliorative effect of Ruellia tuberosa L. on hyperglycemia in type 2 diabetes mellitus and glucose uptake in mouse C2C12 myoblasts

Lin

Zeng

et al. 2018

Food Science & Nutrition

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Ruellia tuberosa L. (RTL) exhibits a wide range of phytochemical activities, for example, on treatment of diabetes mellitus (DM), in Orient. There is, however, few study regarding the effect of RTL on glycemic‐related homeostasis in type 2 DM (T2DM). We investigated the effect of RTL aqueous and ethanolic extracts on hypoglycemia in high‐fat diet (HFD)‐fed plus streptozotocin (STZ)‐induced T2DM rats, and examined the effect of RTL on glucose uptake in tumor necrosis factor‐α‐induced insulin‐resistant mouse C2C12 myoblasts, a mouse skeletal muscle cell line. The administration of 100 or 400 mg kg BW−1 day−1 of RTL aqueous or ethanolic extracts once a day for 4 weeks significantly ameliorated hyperglycemia, hyperinsulinemia, and the insulin resistance (IR) index in diabetic rats. RTL either aqueous or ethanolic extract at a concentration of 25–800 μg/ml significantly improved glucose uptake in insulin‐resistant mouse C2C12 myoblasts, indicating inhibiting the IR in skeletal muscles. These evidences suggest that RTL ameliorates hyperglycemia in HFD/STZ‐induced T2DM rats may be attributed to the alleviation of IR in skeletal muscles.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Ameliorative effect of Ruellia tuberosa L. on hyperglycemia in type 2 diabetes mellitus and glucose uptake in mouse C2C12 myoblasts

Lin

Zeng

et al. 2018

Food Science & Nutrition

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“…BLE antioxidant activity was evaluated on the basis of the scavenging capacity toward a stable DPPH free radical as described previously (22,30,31). A stock solution of BLE (1 mg/ml) was prepared and diluted with methanol to a suitable concentration at 0.5 mg/ml.…”

Section: Preparation Of the Burdock Leaf Extract (Ble)mentioning

confidence: 99%

Inhibitory effect of burdock leaves on elastase and tyrosinase activity

Horng

Chiang

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. Burdock (Arctium lappa L.) leaves generate a considerable amount of waste following burdock root harvest in Taiwan. To increase the use of burdock leaves, the present study investigated the optimal methods for producing burdock leaf extract (BLE) with high antioxidant polyphenolic content, including drying methods and solvent extraction concentration. In addition, the elastase and tyrosinase inhibitory activity of BLE was examined. Burdock leaves were dried by four methods: Shadow drying, oven drying, sun drying and freeze-drying. The extract solution was then subjected to total polyphenol content analysis and the method that produced BLE with the highest amount of total antioxidant components was taken forward for further analysis. The 1,1-diphenyl-2-pycrylhydrazyl scavenging, antielastase and antityrosinase activity of the BLE were measured to enable the evaluation of the antioxidant and skin aging-associated enzyme inhibitory activities of BLE. The results indicated that the total polyphenolic content following extraction with ethanol (EtOH) was highest using the freeze-drying method, followed by the oven drying, shadow drying and sun drying methods. BLE yielded a higher polyphenol content and stronger antioxidant activity as the ratio of the aqueous content of the extraction solvent used increased. BLE possesses marked tyrosinase and elastase inhibitory activities, with its antielastase activity notably stronger compared with its antityrosinase activity. These results indicate that the concentration of the extraction solvent was associated with the antioxidant and skin aging-associated enzyme inhibitory activity of BLE. The reactive oxygen species scavenging theory of skin aging may explain the tyrosinase and elastase inhibitory activity of BLE.In conclusion, the optimal method for obtaining BLE with a high antioxidant polyphenolic content was freeze-drying followed by 30-50% EtOH extraction. In addition, the antielastase and antityrosinase activities of the BLE produced may be aid in the development of skincare products with antiwrinkle and skin-evening properties. IntroductionThe skin is the largest organ of the human body and has essential functions, including protecting internal organs and regulating body temperature. Collagen and elastin, which are naturally occurring structural proteins in the dermis of the skin, maintain the plumpness, flexibility and elasticity of the skin. Skin aging presents as wrinkling, roughness, dryness, decreased elasticity and uneven pigmentation (1,2). In addition to intrinsic aging, extrinsic aging caused by exposure of the skin to solar radiation is an important concern. Reactive oxygen species (ROS) caused by ultraviolet (UV) radiation are clinically associated with specific markers of photoaging, including wrinkling, and increased elastin and collagen damage (2,3). Elastase digests elastin (4,5). Pigmentary changes due to the accumulation of melanin are another major characteristic of photoaging. Tyrosinase is a key enzyme that catalyzes melanin biosynthesis in...

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“…Many plants of genus Ruellia have been used as diuretic, anti-diabetic, anti-pyretic, analgesic, anti-oxidant, anti-hypertensive and gastroprotective [6,7]. Phytochemical constituents which have a wide range of biological activities such as glycoside, alkaloids, flavonoids, triterpenoids have been reported from genus Ruellia [8,9].…”

Section: Introductionmentioning

confidence: 99%