Evaluation of the anti-tumour action and acute toxicity of kosins from Hagenia abyssinica

Woldemariam, Tibebe Z.; Fell, Anthony F.; Linley, P. Alex; Bibby, Michael C.; Phillips, Roger M.

doi:10.1016/0731-7085(92)80080-7

Cited by 18 publications

(11 citation statements)

References 6 publications

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“…However, compound 2 and 3 did not exhibit any toxicity on both the cell lines with 100% viability of cells at their highest concentration of 100 µg/mL. Similar to our findings, strong cytotoxic effects of compound 5 against MAC tumour cells with EC 50 value of 1.5 µg/mL was reported by Woldemariam et al (1992). Compound 2 was reported as nontoxic constituent of Viburnum jucundum (Rios et al, 2001).…”

Section: In Vitro Cytotoxicity Assaysupporting

confidence: 79%

“…Assignment of signals was facilitated by COSY, HSQC and HMBC experiments. The known compounds obtained in this study, phytol acetate (1) (Itoh et al, 2003), triacontanol (2), (Tsai et al, 2007), phytol (3) (Itoh et al, 2003), and alpha kosin (5) (Woldemariam et al, 1992) were identified by comparison of their physical and spectroscopic data with literature reports.…”

Section: Identification Of Isolated Compoundsmentioning

confidence: 99%

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The potential of Leucosidea sericea against Propionibacterium acnes

Sharma

Kishore

Hussein

et al. 2014

Phytochemistry Letters

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Highlights:· Significant anti-bacterial and antioxidant activity displayed by crude extract of Leucosidea sericea and isolated compound. · Electron microscopy studies proved the lethal effects of plant extract and compound 5 on the cells of P. acnes. 2 Graphical abstractAntibacterial bioassay of ethanol extract of Leucosidea sericea and isolated compounds against Propionibacterium acnes. · AbstractThe present study reports on the potential of Leucosidea sericea addressing acne vulgaris.Four known compounds namely, phytol acetate, triacontanol, phytol and alpha kosin and one new compound namely, (E)-3,7,11,15-tetramethylheptadec-2-ene-1,1 7-diol have been isolated for the first time from this plant. The ethanol extract of leaves and one of the isolated compounds, alpha kosin exhibited significant minimum inhibitory concentration (with MIC values 15.7 and 1.9 µg/mL, respectively) against acne inducing bacteria, Propionibacterium acnes. Moreover, the transmission electron micrographs showed the efflux of intracellular content of the cells of P. acnes caused by plant extract and alpha kosin. The ethanol extract of L. sericea exhibited significant anti-inflammatory activity by suppressing interleukin 8 (IL 8) and tumour necrosis factor (TNF α) in coculture of human U937 cells and heat killed P.acnes at concentrations of 25.0, 12.5 and 6.2 µg/mL.

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Section: In Vitro Cytotoxicity Assaysupporting

confidence: 79%

Section: Identification Of Isolated Compoundsmentioning

confidence: 99%

The potential of Leucosidea sericea against Propionibacterium acnes

Sharma

Kishore

Hussein

et al. 2014

Phytochemistry Letters

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“…The species thus became incorporated in the European Pharmacopoeia (Lounasmaa et al 1973) and several papers have since reported on the medical aspects of kosins which have e.g. antitumour activity (Abegaz et al 1999;Lounasmaa et al 1974;Woldemariam et al 1992). Although no longer marketed in Europe, H. abyssinica is still widely used in Ethiopia for the treatment of tapeworm.…”

Section: Introductionmentioning

confidence: 97%

Analysis of genetic diversity in the endangered tropical tree species Hagenia abyssinica using ISSR markers

Feyissa

Nybom

Bartish

et al. 2007

Genet Resour Crop Evol

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Genetic diversity within and among 12 populations of the dioecious tropical tree species Hagenia abyssinica (Bruce) J.F. Gmel. in Ethiopia was examined with eight inter simple sequence repeat (ISSR) primers. A total of 104 clearly scorable bands were generated, among which 84 (81%) were polymorphic. Jaccard similarity coefficient was calculated for pairwise comparisons among all 120 individuals and ranged from 0.30 to 0.88 while average withinpopulation similarity ranged from 0.53 to 0.66. Within-population variability was estimated as percentage polymorphic loci (ranging from 52% to 87%), Shannon's information index (0.30-0.50) and Nei's genetic diversity (0.21-0.35). The highest variability values were obtained for one recently planted population and for one wild population growing in an undisturbed primary forest area. Significant overall differentiation among populations was detected by both Shannon's information index (0.26) and G ST (0.25). Relatedness among samples was estimated with a principal coordinate analysis, and relatedness among populations was estimated with a cluster analysis (UPGMA). A Mantel test indicated a significant association between genetic and geographic distances, and an autocorrelation analysis showed significant evidence of gene flow over distances up to 30 km. This study is the first of its kind for H. abyssinica, which has decreased recently in Ethiopia and now must be regarded as an endangered species. Both within-population and between-population diversity estimates are typical of outcrossing, longlived and late successional species, suggesting that recent anthropogenic disturbances have not yet had much impact on population genetic parameters. DNA marker data can, however, be used to identify the most suitable sites for in situ conservation and for collection of material for establishment of genebanks and plant improvement programs.

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“…Since the constituents are highly poisonous and hepatotoxic, the drug is now obsolete in Europe, even though Kosso shows remarkable anthelmintic activity and is still used widely in Ethiopia as taenicide. In addition to its anthelmintic activity kosins also show anti-cancer activity against murine adeno-carcinoma cell lines (MAC) in vitro and in vivo [11]. These cell lines behave very similarly to tumors of the human colon.…”

Section: Introductionmentioning

confidence: 99%

“…Children infested with the parasite resist taking the drug and are sometimes punished by their parents in order to force them to swallow the bitter concoction. Although the nonpolar kosotoxin is believed to be the active principle, some contributing components may well exist in the crude water extract of Hagenia abyssinica and influence its activity [11]. The occurrence of phloroglucinol glycosides possessing a variety of biological activities have been reported from other natural sources [18].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Constituents and Anthelmintic Properties of Hagenia abyssinica

Thomsen

2012

Sci. Pharm.

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The dried female flowers of Hagenia abyssinica (Bruce) J. F. Gmel. (Rosaceae) are traditionally used as an anthelmintic remedy in Ethiopia and formerly were incorporated into the European Pharmacopoeia. One-, two- and tricyclic phloroglucinol derivatives (kosins) were suggested to be the active principles. However, polar constituents may also contribute to the activity. Therefore, we investigated for the first time the polar constituents. We isolated typical Rosaceae constituents such as quercetin 3-O-β-glucuronide, quercetin 3-O-β-glucoside and rutin. Polar kosin glycosides or derivatives could not be detected.The anthelmintic activity of fractions of different polarity were tested against the blood fluke Schistosoma mansoni, the liver flukes Clonorchis sinensis and Fasciola hepatica and the intestinal fluke Echinostoma caproni. The anthelmintic activity decreased with increasing polarity of the tested fractions. ESI-MS investigations indicated the predominant occurrence of kosins in the active fractions.Using the anthelmintic active extracts of Hagenia abyssinica we developed a simple, inexpensive bioassay against the non-parasitic nematode Caenorhabditis elegans, which can be used as an initial screening procedure for anthelmintic properties of crude extracts of plants or fungi. The anthelmintic activity of test extracts against the model organism was determined in a microtiter plate assay by enumeration of living and dead nematodes under a microscope.

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Evaluation of the anti-tumour action and acute toxicity of kosins from Hagenia abyssinica

Cited by 18 publications

References 6 publications

The potential of Leucosidea sericea against Propionibacterium acnes

The potential of Leucosidea sericea against Propionibacterium acnes

Analysis of genetic diversity in the endangered tropical tree species Hagenia abyssinica using ISSR markers

Characterization of Constituents and Anthelmintic Properties of Hagenia abyssinica

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