Characterization of Constituents and Anthelmintic Properties of Hagenia abyssinica

Thomsen, Henrieke

doi:10.3797/scipharm.1109-04

Cited by 35 publications

(23 citation statements)

References 33 publications

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“…The main nonpolar constituents of the flowers are protokosin and kosotoxin (8) (Lounasma et al, 1974). Recently, we investigated for the first time the polar constituents of the female flowers using the non-parasitic nematode Caenorhabditis elegans for the anthelmintic assay and found out that the polar compounds, which are mainly flavonoids, are not responsible for the anthelimintic activities, confirming earlier assumptions that the activity is due to the nonpolar kosins, such as kosotoxin (Thomsen et al, 2012).…”

Section: Hagenia Abyssinica (Rosaceae) Koso (Amharic)supporting

confidence: 63%

Unique Bioresources from Ethiopia for Food, Medicine and Cosmetics

Dagne

2014

Novel Plant Bioresources

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Section: Hagenia Abyssinica (Rosaceae) Koso (Amharic)supporting

confidence: 63%

Unique Bioresources from Ethiopia for Food, Medicine and Cosmetics

Dagne

2014

Novel Plant Bioresources

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“…Also in previous assay developments, we found that GFP does not always cease to fluorescence if the expressing organism dies. 18 This was not yet observed for YFP. Thus, for these two reasons (background and stability) we decided for YFP.…”

Section: Assay Developmentmentioning

confidence: 83%

A fluorescence-based bioassay for antibacterials and its application in screening natural product extracts

et al. 2015

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The reliable assessment of the biological activity of a minor component embedded in a complex matrix of several hundred compounds is a difficult but common task in the search for natural product-based antibiotics, for example, by bioassay-guided fractionation. To quantify the antibiotic properties, it is necessary to assess the cell viability. Direct measurements use CFU counts, OD measurements or detection via fluorescent or reducible dyes. However, natural extracts often already possess intrinsic dye, fluorescent, reducing or protein denaturing properties, or they contain insoluble compounds or general protein-binding (tanning) polyphenols as disturbing features, while at the same time very little of the selective antibiotic sought after is present. A promising alternative is provided by intrinsically produced bright fluorescent proteins. In this paper, a rapid, robust and concentration-dependent assay for screening antibiotics with genetically modified mutants of Bacillus subtilis 168 (PabrB-iyfp) is presented. The Gram-positive bacteria exhibit a native fluorescence during their exponential growth phase due to the expression of improved yellow fluorescent protein. To demonstrate the applicability in the field of natural product research, several compounds and extracts were screened for antibacterial activity, with an emphasis on those from the fungal genus Hygrophorus (waxy caps).

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“…However, it generally belongs to those phenolic substances that are being used in veterinary and human medicine with low toxicity (EMEA/MRL/046/95-FINAL). PGL and its chemical derivatives have been previously tested for in vivo toxicity in several animal model systems, including Caenorhabditis elegans and zebrafish [57][58][59] . Given our previously published results and the high concentrations required for organ or systemic toxicity in either zebrafish or mice and rats, we herein considered the use of PGL in vivo doses of a similar millimolar (mM) scale 59,60 .…”

Section: Discussionmentioning

confidence: 99%

Exploitation of Drosophila Choriogenesis Process as a Model Cellular System for Assessment of Compound Toxicity: the Phloroglucinol Paradigm

Keramaris¹,

Κωνσταντόπουλος²,

Margaritis³

et al. 2020

Sci Rep

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Phloroglucinol (1,3,5 tri-hydroxy-benzene) (PGL), a natural phenolic substance, is a peroxidase inhibitor and has anti-oxidant, anti-diabetic, anti-inflammatory, anti-thrombotic, radio-protective, spasmolytic and anti-cancer activities. PGL, as a medicine, is administered to patients to control the symptoms of irritable bowel syndrome and acute renal colic, in clinical trials. PGL, as a phenolic substance, can cause cytotoxic effects. Administration of PGL up to 300 mg/kg (bw) is well tolerated by animals, while in cell lines its toxicity is developed at concentrations above the dose of 10 μg/ml. Furthermore, it seems that tumor or immortalized cells are more susceptible to the toxic power of PGL, than normal cells. However, studies of its cytotoxic potency, at the cellular level, in complex, differentiated and metamitotic biological systems, are still missing. In the present work, we have investigated the toxic activity of PGL in somatic epithelial cells, constituting the follicular compartment of a developing egg-chamber (or, follicle), which directs the choriogenesis (i.e. chorion assembly) process, during late oogenesis of Drosophila melanogaster. our results reveal that treatment of in vitro growing Drosophila follicles with PGL, at a concentration of 0.2 mM (or, 25.2 μg/ml), does not lead to follicle-cell toxicity, since the protein-synthesis program and developmental pattern of choriogenesis are normally completed. Likewise, the 1 mM dose of PGL was also characterized by lack of toxicity, since the chorionic proteins were physiologically synthesized and the chorion structure appeared unaffected, except for a short developmental delay, being observed. In contrast, concentrations of 10, 20 or 40 mM of PGL unveiled a dose-dependent, increasing, toxic effect, being initiated by interruption of protein synthesis and disassembly of cell-secretory machinery, and, next, followed by fragmentation of the granular endoplasmic reticulum (ER) into vesicles, and formation of autophagic vacuoles. Follicle cells enter into an apoptotic process, with autophagosomes and large vacuoles being formed in the cytoplasm, and nucleus showing protrusions, granular nucleolus and condensed chromatin. PGL, also, proved able to induce disruption of nuclear envelope, activation of nucleus autophagy (nucleophagy) and formation of a syncytium-like pattern being produced by fusion of plasma membranes of two or more individual follicle cells. Altogether, follicle cell-dependent choriogenesis in Drosophila has been herein presented as an excellent, powerful and reliable multi-cellular, differentiated, model biological (animal) system for drug-cytotoxicity assessment, with the versatile compound PGL serving as a characteristic paradigm. In conclusion, PGL is a substance that may act beneficially for a variety of pathological conditions and can be safely used for differentiated somatic-epithelial-cells at clinically low concentrations. At relatively high doses, it could potentially induce apoptotic and autophagic cell death, thus being likely ...

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Characterization of Constituents and Anthelmintic Properties of Hagenia abyssinica

Cited by 35 publications

References 33 publications

Unique Bioresources from Ethiopia for Food, Medicine and Cosmetics

Unique Bioresources from Ethiopia for Food, Medicine and Cosmetics

A fluorescence-based bioassay for antibacterials and its application in screening natural product extracts

Exploitation of Drosophila Choriogenesis Process as a Model Cellular System for Assessment of Compound Toxicity: the Phloroglucinol Paradigm

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