Evaluation of the Anti-angiogenic Activity of Saponins from Maesa lanceolata by Different Assays

Abstract

Angiogenesis, in which a vascular network is established from pre-existing vessels, is a complex multistep process. Mechanisms underlying angiogenesis can be investigated using a variety of in vitro, ex vivo and in vivo approaches. Evaluation of several promising plants and plant metabolites, including terpenoids, revealed promising anti-angiogenic activity. Since the maesasaponins displayed anti-angiogenic activity in the chick chorioallantoic membrane (CAM) assay, their activity was further investigated in several test systems. The rat aorta ring assay was compared with the placental vein assay and then selected for the ex vivo investigation of the saponins. Besides their effect on the viability of HUVEC, the anti-angiogenic capacity of the compounds was also investigated in an in vivo zebrafish assay. The activity of the saponins in the viability assay was more pronounced than in the rat aorta ring assay and similar to the effect observed in the CAM assay. The use of different test systems, however, implies different results in the case of saponins.

Characterization of vitamin–cisplatin-loaded chitosan nano-particles for chemoprevention and cancer fatigue

“…Therefore, the synergistic combinations can be used for advanced drug delivery purposes. Anti-angiogenesis activity along with CAM assay has also been used to study the action of nano-particles on blood vessels formation (Foubert et al, 2012). The formation of wide, thick vascular zones indicates the anti-angiogenic activity of F4 nanoparticles on the blood vessels.…”

“…An anti-angiogenesis activity was conducted using the chick chorioallantoic membrane (CAM) assay (Foubert et al, 2012). The 7-d-old fertilized eggs were purchased, disinfected with 70% ethanol, and transferred to an incubator (GENEI, Bangalore, India) at 37 C with 75% humidity; they were kept in these optimal growth conditions for 24 h. To ensure equal distribution of the blood vessels, the eggs were turned over every 6 h. After 24 h, the eggs were wiped with ethanol (70%) to prevent any mild infections, and then the eggshells were sawed with a metal saw blade.…”

Aureobasidium pullulans produced β-glucan is effective to enhance Kurosengoku soybean extract induced Thrombospondin-1 expression

“…As THBS1 is known to be a protein exhibiting anti-angiogenic activity 26 , 27 , and anti-angiogenic activity has been established in the soybean isoflavone, genistein 44 , 45 . In addition, anti-angiogenic activity of soy saponins has also been reported 46 . As shown in Fig.…”

Inhibiting Angiogenesis by Anti-Cancer Saponins: From Phytochemistry to Cellular Signaling Pathways

“…The structure–activity relationship studies of Pulsatilla saponins showed that the presence of the carboxyl group at the C-28 position was necessary for the anti-cancer activities of these saponins, although it increased their hemolytic effects [ 204 , 205 , 206 , 207 , 208 ]. Platycodin D ( Figure 5 ) [ 209 , 210 ], julibroside J8 [ 211 ], maesasaponins (I-VII.1) [ 212 ], astragaloside IV [ 213 ], capilliposide B ( Figure 5 ) [ 214 ], raddeanin A ( Figure 5 ) [ 215 , 216 ], and gleditsiosides B [ 217 ] are other triterpenoid saponins with anti-angiogenesis activities described in Table 1 . Triterpenoid saponins showed anti-angiogenesis activities via blocking the pro-angiogenesis mediators, including VEGF and HIF-1α, as well as suppressing the PI3K/Akt, MAPKs, and NF-κB as stimulation angiogenesis cellular signaling pathways.…”