Evaluation of SWCNTs-loaded DNA vaccine encoding predominant antigen epitope VP4-3 against type II GCRV

Zheng, Yonghua; Qiu, De-Kui; Guo, Zi‐Rao; Gong, Yu-Ming; Wang, Gao‐Xue; Zhu, Bin

doi:10.1016/j.aquaculture.2020.736197

Cited by 13 publications

(6 citation statements)

References 55 publications

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“…This fully demonstrates the superiority of SWCNTs-based vaccines. The studies of Zhu et al, Hu et al and Zheng et al also confirmed this point (Hu et al, 2020;Zheng et al, 2021;Zhu et al, 2014;Zhu, Liu, Gong, et al, 2015).…”

Section: Svcv Has Aroused Widespread Interest Among Researchers Inmentioning

confidence: 77%

Optimization of immunization procedure for SWCNTs‐based subunit vaccine with mannose modification against spring viraemia of carp virus in common carp

Gong

Zhang

et al. 2021

Journal of Fish Diseases

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Immersion vaccination of single-walled carbon nanotubes loaded with mannosemodified glycoprotein (SWCNTs-MG) vaccine has been proved to be effective in preventing spring viraemia of carp virus (SVCV). Immunization procedure has immense consequence on the immune effect of the immersion vaccine. However, immunization procedure optimization for SWCNTs-MG vaccine against SVCV has not been reported. In this study, accordingly, a full-factor experiment was designed to optimize the immunization procedure of SWCNTs-MG vaccine by three aspects of vaccine dose (30 mg/L, 40 mg/L and 50 mg/L), immunization density (8 fish L −1 , 24 fish L −1 and 48 fish L −1 ) and immunization time (6, 12 and 24 hr). Furthermore, we used the immunization group (A1B2C1, 30 mg/L, 24 fish L −1 and 6 hr) in the previous study as a positive control (PC) to evaluate the immunization effect optimized conditions from the expression of immune-related genes and relative percentage survival (RPS). At 28 days post-vaccination (DPV), common carps were intraperitoneal injected SVCV challenged test indicated that the A1B2C2 group (30 mg/L, 24 fish L −1 , 12 hr) displayed superiority of protective efficacy compare with other groups and the RPS with 77.9%, which was 15.6% higher than the PC group of RPS with 62.3%.Moreover, the expression of immune-related genes such as IL-10, CD4 and MHC-II was also significantly higher than PC group. The specific experimental flow chart is shown in Figure 1. Conclusively, these results demonstrated that vaccine dose, immunization density and immunization time are 30 mg/L, 24 fish L −1 and 12 hr, which is the more appropriate immunization programme with juvenile carp for SWCNTs-MG vaccine. This study provides a profitable reference for improving the immune efficiency of aquatic immersion vaccine.

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Section: Svcv Has Aroused Widespread Interest Among Researchers Inmentioning

confidence: 77%

Optimization of immunization procedure for SWCNTs‐based subunit vaccine with mannose modification against spring viraemia of carp virus in common carp

Gong

Zhang

et al. 2021

Journal of Fish Diseases

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“…In addition, the plasma was stored at −20°C before using. The ELISA method was prepared as described in our previous study (Zheng et al, 2021). Briefly, the purified MCP protein was used as the antigen, and bound antibodies were anti‐His tag IgG antibody and goat‐mouse IgG antibody, which were diluted at 1:1500 with PBST.…”

Section: Methodsmentioning

confidence: 99%

Antigenic epitope screening and functional modification of mannose enhance the efficacy of largemouth bass virus subunit vaccines

Jia

Xia

Jiang

et al. 2022

Journal of Fish Diseases

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Major capsid protein (MCP) can be used as a subunit vaccine against largemouth bass virus (LMBV). However, subunit vaccines usually have low immunogenicity. Here, to identify the major immunogenicity determinant region of the MCP gene, we truncated the MCP of the LMBV gene into four parts (MCP‐1, MCP‐2, MCP‐3 and MCP‐4). Enzyme‐linked immunosorbent assay (ELISA) was used to identify the antigenicity of these four truncated MCP proteins. Then, the highly antigenic truncated protein was modified with mannose and connected with functionalized single‐walled carbon nanotubes (SWCNTs) as carriers. Largemouth basses were immunized by bath immersion, challenged with LMBV on the 28th day after immunization and evaluated for related immune indicators. The results indicated that the MCP‐2 protein could induce a higher antibody titre than the other truncated MCP proteins. We found that the levels of immune‐related genes (TNF‐α, CD40, IgM, IFNγ and IL‐10) in the spleen and kidney were significantly increased in the MCP‐2 and MCP‐2‐Man groups. ELISA results showed that the antibody content in the serum increased significantly in the MCP‐2 group 7 days post‐vaccination and increased with days in all the vaccinated groups, with the highest observed on the 21st day. Notably, the MCP‐2‐Man vaccine (10 mg L−1) showed durability of immunoprotection efficacy that could protect largemouth basses from LMBV challenge, and the immune protection rate reached 78.94%. These results suggest that MCP‐2 might be the major immunogenicity determinant region of LMBV and that the mannose‐modified MCP‐2 vaccine can induce stronger adaptive immune responses.

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“…Grass carp bath immunized with SWCNT vaccine (10 mg L À1 ) for 12 h exhibited enhanced immune responses and the highest RPS of 88.33% against GCRC-II challenge. Furthermore, SWCNTs were used as delivery vectors for pvDNA-VP4-3 subunit vaccine against GCRV-II ( Zheng et al 2021). Immunization (i.m) of grass carp with SWCNT-pvDNA-VP4-3 markedly increased the serum antibody level and conferred higher protection (RPS: 74%) in fish against GCRV-II challenge (Zheng et al 2021).…”

Section: Cnts Against Viral Infections In Aquaculturementioning

confidence: 99%

“…Furthermore, SWCNTs were used as delivery vectors for pvDNA-VP4-3 subunit vaccine against GCRV-II ( Zheng et al 2021). Immunization (i.m) of grass carp with SWCNT-pvDNA-VP4-3 markedly increased the serum antibody level and conferred higher protection (RPS: 74%) in fish against GCRV-II challenge (Zheng et al 2021). The outcomes of these studies indicated that SWCNTs increased the retention time of DNA vaccines in the fish body, improving its expression intensity.…”

Section: Cnts Against Viral Infections In Aquaculturementioning

confidence: 99%

Applications of carbon nanotubes and polymeric micro‐/nanoparticles in fish vaccine delivery: progress and future perspectives

Giri

Kim

Kang

et al. 2021

Reviews in Aquaculture

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Newly evolving strains of pathogenic microorganisms in aquaculture are posing formidable challenge in controlling and/or eradication of diseases affecting aquaculture. Many research studies have aimed to address this challenge, and the delivery of vaccines using biocompatible nano-delivery systems is widely investigated. Nanoparticles (NPs) have tremendous potential for application as delivery vehicles of novel vaccines. At present, various polymeric and non-polymeric NPs are utilized to deliver vaccines in aquatic species. This review comprehensively discusses recent research advancements in the utilization of carbon nanotubes and polymeric micro-/nanoparticles for the delivery of drug/nutrients to control fish diseases. Further, this review compares the potentials of various vaccine delivery systems utilized to control fish pathogens. The necessity of non-toxic and eco-friendly strategies for aquaculture has also been emphasized in this review.

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Evaluation of SWCNTs-loaded DNA vaccine encoding predominant antigen epitope VP4-3 against type II GCRV

Cited by 13 publications

References 55 publications

Optimization of immunization procedure for SWCNTs‐based subunit vaccine with mannose modification against spring viraemia of carp virus in common carp

Optimization of immunization procedure for SWCNTs‐based subunit vaccine with mannose modification against spring viraemia of carp virus in common carp

Antigenic epitope screening and functional modification of mannose enhance the efficacy of largemouth bass virus subunit vaccines

Applications of carbon nanotubes and polymeric micro‐/nanoparticles in fish vaccine delivery: progress and future perspectives

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