Evaluation of¹³¹I-Pentamidine for scintigraphy of experimentallyLeishmania tropica-infected hamsters

Abstract

We aimed to assess the ability of (131)I-Pentamidine scintigraphy to detect the lesions of Leishmania tropica infection. An experimental model of cutaneous leishmaniasis was developed. The presence of cutaneous leishmaniasis was confirmed. Pentamidine was radioiodinated with (131)I. The radiolabeled pentamidine was validated by the requisite quality control tests to check its radiolabeling efficiency, in vitro stability. (131)I-Pentamidine (activity: 18.5 MBq/100 µl) was injected intracardiacally into infected hamsters. Static whole body images of the hamsters were acquired under the gamma camera at 5 and 30 min, 2, 6 and 24 h following the administration. On the scintigrams, anatomically adjusted regions of interest (ROIs) were drawn over the right feet (target) and left feet (not-target) and various organs. Accumulation of (131)I-Pentamidine at sites of infection is expressed as the target to non-target (T/NT) ratio. The results T/NT ratio decreased with time. In concluding the (131)I-Pentamidine has poor sensitivity in detection of L. tropica infection.

Awareness of the existence of Leishmaniasis as Protozoan Zoonosis in Indonesia

“…Menurut tinjauan sejarah evolusi Leishmaniasis oleh Akhoundi et al (2016) Sebagai binatang peliharaan, anjing dapat dengan mudah dikontrol oleh dokter hewan untuk pengendalian leishmanisis, namun anjing liar atau spesies rubah seperti Lycalopex vetulus, cerdocyonthus sulit dikontrol dan dapat menyebabkan wabah. Beberapa jenis rodensia seperti Didelphis albiventris (opossum), Mus musculus (tikus domestik), Microtus socialis, Rattus rattus (tikus hitam), Cercomys cunicularius (tikus liar), Mesocricetus auratus (hamster Syria) dikenal sebagai penyebar Leishmaniasis di Amerika, Afrika dan Asia (Inceboz et al 2014;Pourmohammadi et al 2008).…”

Epidemiology and Ecology of Leishmaniasis

“…Different rodents such as Didelphis albiventris (opossum), Mus musculus (domestic mouse), Microtus socialis, Rattusrattus (black rat), Cercomys cunicularius (wild rat), Mesocricetus auratus (Syrian hamsters) in America, Africa and Asia lead to spread of leishmaniasis [47][48][49][50][51].…”

Current Topics in Neglected Tropical Diseases

“…Twenty-five percent of CHIKV cases have been estimated to result in chronic inflammatory rheumatism, although this study did not address the issue of differences between genotypes [50]. Other symptoms, such as chronic pain, mental health issues, and nonspecific manifestations, are also common in this phase [51][52][53][54], with arthralgia and arthritis with pain accompanied by articular edema or morning joint stiffness being the most frequent clinical manifestations [55].…”

“…Among 19 patients treated with MTX, 13 had a good clinical response (68.4%). Among these patients, 54.1% were positive for rheumatoid factor, and 28.6% had anti-cyclic citrullinated peptide antibodies [50]. As such, many of the patients were diagnosed with seronegative RA, which can closely mimic the signs and symptoms of CCA, and which might respond to similar treatments.…”