Evaluation of sulfobetaine-type zwitterionic stationary phases for ion Chromatographic separation using water as a mobile phase

Umemura, Tomonari; Kamiya, Shinji; Itoh, Akihide; Chiba, Koichi; Haraguchi, Hiroki

doi:10.1016/s0003-2670(97)00222-5

Cited by 25 publications

(22 citation statements)

References 14 publications

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“…In EIC using a sulfobetaine-type zwitterionic stationary phase and water eluent, the elution order of anions is consistent with Hofmeister series related to the state of hydration, as reported in previous paper [18]. This fact means that the differences in hydration energy play an important role in separation selectivity for inorganic anions.…”

Section: Theoreticalsupporting

confidence: 89%

Elucidation of Ion-Pairing Elution Behavior of Anions and Cations in Electrostatic Ion Chromatography Using Water as a Mobile Phase

Umemura

2019

Chromatography

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Peculiar ion-pairing elution behavior of analyte anions with counter cations in electrostatic ion chromatography (EIC) using water as a mobile phase was theoretically elucidated based on the Donnan membrane equilibrium principle and charge balance condition. New parameters, f anion and f cation , containing a fluctuating coefficient, x, which corresponded to capacity factors, were derived from the Donnan equation. The f anion and f cation of the constituent anion and cation in ion-pairing elution were connected with each other by the x, and the capacity factor, k ion pair (of co-eluting anion and cation) could be predicted by solving the relation of f anion = f cation for x. The elution volumes for various ion pairs thus semiempirically calculated were in good agreement with those experimentally obtained. In addition, the selective ion-pairing formation in EIC could reasonably be explained by applying the parameters to a modified classical plate theory.

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Section: Theoreticalsupporting

confidence: 89%

Elucidation of Ion-Pairing Elution Behavior of Anions and Cations in Electrostatic Ion Chromatography Using Water as a Mobile Phase

Umemura

2019

Chromatography

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“…, where the ICP-MS was also used as the element-selective detector. The CHAPS-coated ODS column was prepared by the dynamic coating method, as described in previous papers [4,[19][20][21], and has unique characteristics for simultaneous separation of large and small ions/molecules, as mentioned later. The size exclusion chromatography (SEC) column used for protein separation was of Superose 12HR 10/300 GL (Amersham Bioscience, Piscataway, NJ, USA) with a molecular permeation range of 1-300 kDa, whose calibration for molecular weights (MWs) was performed using standard proteins, as usual [22,23].…”

Section: Instrumentationmentioning

confidence: 99%

“…Two separation columns were employed for molecular separation; one was a CHAPS (zwitterionic surfactant)-coated ODS column and the other was an SEC column. As described in previous papers [19][20][21][22], the CHAPS-coated ODS column has unique characteristics for simultaneous separation of ions/small molecules and large molecules (e.g., proteins), which provides useful information for metals binding or not binding with proteins.…”

Section: Fractionation Analysis Of Protein-binding Elements In Salmonmentioning

confidence: 99%

Metallomics study on all-elements analysis of salmon egg cells and fractionation analysis of metals in cell cytoplasm

Ishii

Hasegawa

Matsuura

et al. 2008

Pure and Applied Chemistry

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Salmon egg cells were the subject of investigation for all-elements analysis in a single biological cell, where the elements in egg cells were simultaneously determined by inductively coupled plasma atomic emission spectrometry (ICP-AES) and inductively coupled plasma mass spectrometry (ICP-MS) after microwave-assisted acid digestion. As a result, 74 elements among 78 stable isotope elements were determined or detected, although H, C, N, and O were determined separately by conventional CHN elemental analysis. In addition, the survey of protein-binding metallic elements as well as metalloid elements in salmon egg cell cytoplasm was performed by the fractionation analysis with a hyphenated system of highperformance liquid chromatography (HPLC)/ICP-MS using a 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate (CHAPS)-coated octadecylsilyl silica (ODS) column and a size exclusion chromatography (SEC) column.

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“…6 Besides, some stationary phases, where there is no positive/ negative charge, could achieve the separation of ions by dynamically coating ionic reagents, such as ion-pairs reagents or surfactants. [7][8][9][10] It was also found that some inorganic anions could be separated on the C30 stationary phase by the hydrophobic interaction. 11 The separation of inorganic anions in the partition mode was observed by the C30 stationary phase coated by poly(ethylene glycol) (PEG).…”

Section: Introductionmentioning

confidence: 99%

Retention Behavior of Inorganic Anions in Hydrophilic Interaction Chromatography

2017

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Evaluation of sulfobetaine-type zwitterionic stationary phases for ion Chromatographic separation using water as a mobile phase

Cited by 25 publications

References 14 publications

Elucidation of Ion-Pairing Elution Behavior of Anions and Cations in Electrostatic Ion Chromatography Using Water as a Mobile Phase

Elucidation of Ion-Pairing Elution Behavior of Anions and Cations in Electrostatic Ion Chromatography Using Water as a Mobile Phase

Metallomics study on all-elements analysis of salmon egg cells and fractionation analysis of metals in cell cytoplasm

Retention Behavior of Inorganic Anions in Hydrophilic Interaction Chromatography

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