Retention Behavior of Inorganic Anions in Hydrophilic Interaction Chromatography

Takayama, Nobuyuki; Lim, Lee Wah; Takeuchi, Toyohide

doi:10.2116/analsci.33.619

Cited by 13 publications

(9 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These ions are known to interfere with the electrospray ionization and affect the sensitivity in mass spectrometry [25]. This effect can be incremental in HILIC separation since these ions can be retained by the polar stationary phase and interact with the mobile phase additive [26]. To investigate the effect of matrix inorganic ions in untargeted HILIC-MS analysis, chromatographic separation and ionization of analytes of interest were compared with sodium chloride solution spiked with the standard mix.…”

Section: Matrix Effectmentioning

confidence: 99%

Systematic Evaluation of HILIC Stationary Phases for Global Metabolomics of Human Plasma

et al. 2022

View full text Add to dashboard Cite

Polar hydrophilic metabolites have been identified as important actors in many biochemical pathways. Despite continuous improvement and refinement of hydrophilic interaction liquid chromatography (HILIC) platforms, its application in global polar metabolomics has been underutilized. In this study, we aimed to systematically evaluate polar stationary phases for untargeted metabolomics by using HILIC columns (neutral and zwitterionic) that have been exploited widely in targeted approaches. To do so, high-resolution mass spectrometry was applied to thoroughly investigate selectivity, repeatability and matrix effect at three pH conditions for 9 classes of polar compounds using 54 authentic standards and plasma matrix. The column performance for utilization in untargeted metabolomics was assessed using plasma samples with diverse phenotypes. Our results indicate that the ZIC-c HILIC column operated at neutral pH exhibited several advantages, including superior performance for different classes of compounds, better isomer separation, repeatability and high metabolic coverage. Regardless of the column type, the retention of inorganic ions in plasma leads to extensive adduct formation and co-elution with analytes, which results in ion-suppression as part of the overall plasma matrix effect. In ZIC-c HILIC, the sodium chloride ion effect was particularly observed for amino acids and amine classes. Successful performance of HILIC for separation of plasma samples with different phenotypes highlights this mode of separation as a valuable approach in global profiling of plasma sample and discovering the metabolic changes associated with health and disease.

show abstract

Section: Matrix Effectmentioning

confidence: 99%

Systematic Evaluation of HILIC Stationary Phases for Global Metabolomics of Human Plasma

et al. 2022

View full text Add to dashboard Cite

show abstract

“…It is also a reference method for the analysis of carbohydrates, , amino acids, small peptides, and glycopeptides . It can also be a suitable replacement strategy for ion exchange in the analysis of small inorganic ions . However, progress in HILIC is clearly driven by the needs of bioanalysis and, above all, metabolomics, where HILIC has been established as a reference strategy.…”

Section: Hilic For Polar and Ionizable Compoundsmentioning

confidence: 99%

Recent Advances in Chromatography for Pharmaceutical Analysis

et al. 2018

View full text Add to dashboard Cite

“…[3][4][5][6][7][8] In addition, with the downsizing of ion chromatographs, mini-columns or capillary columns are employed to IC. [9][10][11][12][13][14][15] Considering the above background, we came up with use of a guard column in IC. Guard columns packed with the same ionexchange resin as the IC separation column are generally used to remove soluble impurities and extend the lifespan of the resin in the separation column.…”

Section: Introductionmentioning

confidence: 99%

Utilization of Anion-exchange Guard Column as an Ion Chromatographic Column of Anions Including Application to Simultaneous Separation of Anions and Cations

et al. 2019

View full text Add to dashboard Cite

This study demonstrated that a guard column containing anion-exchange resin has the potential for use as a separation column for acid eluent. Specifically, a 1-cm long anion-exchange guard column with a 4.6-mm internal diameter provided good separation of monovalent inorganic anions, by elution of 8 mM tartaric acid or 4 mM malic acid. Using the guard column with acid eluent could be applied to evaluation of nitrite and nitrate ions in mountain and urban river water samples. When the guard column was connected in front of a cation-exchange separation column (15 cm long × 4.6 mm internal diameter) in a series, the system provided simultaneous separation of anions and cations in eluent of 8 mM tartaric acid and 0.5 mM 18-crown-6 ether by a single injection.

show abstract

Retention Behavior of Inorganic Anions in Hydrophilic Interaction Chromatography

Cited by 13 publications

References 22 publications

Systematic Evaluation of HILIC Stationary Phases for Global Metabolomics of Human Plasma

Systematic Evaluation of HILIC Stationary Phases for Global Metabolomics of Human Plasma

Recent Advances in Chromatography for Pharmaceutical Analysis

Utilization of Anion-exchange Guard Column as an Ion Chromatographic Column of Anions Including Application to Simultaneous Separation of Anions and Cations

Contact Info

Product

Resources

About