Evaluation of silibinin on the viability, migration and adhesion of the human prostate adenocarcinoma (PC‐3) cell line

Abstract

Prostate cancer (PCA) is the most common cancer diagnosed in men and the second most common cause of death due to cancers after lung cancer. Metastasis of cancer cells involves multiple processes and various cytophysiological changes, including changed adhesion capability between cells and extracellular matrix (ECM) and damaged intercellular interaction. Silibinin, a naturally occurring flavonoid antioxidant found in the milk thistle, has recently been shown to have potent antiproliferative effect against various malignant cell lines. In the present study, PC-3 cells were incubated with various concentrations of silibinin for different times; then, cell cytotoxicity, cell adhesion and cell motility were assessed using MTT assay, cell-matrix adhesion assay and cell migration assay, respectively. The results showed that silibinin exerted a dose- and time-dependent inhibitory effect on the viability, motility and adhesion of highly metastatic PC-3 cells. These observations indicate that silibinin can probably inhibit metastasis in PCA.

Cisplatin Induces Up-Regulation of KAI1, a Metastasis Suppressor Gene, in MCF-7 Breast Cancer Cell Line

“…At concentrations of 10, 20, 40, 80 and 100 µM of cisplatin MCF-7cell viability was reduced to 84.0 ± 12.4 (statistically insignificant, p > 0.05), 67.2 ± 12.4 (p < 0.05), 35.6 ± 3.4 (p <0.001) and 30.7 ± 2.5 % (p < 0.001), respectively. LC 50 of cisplatin after 24 h was 143.4 ± 14.6 µg/ml, as reported in our previous study [10].…”

“…The cells were incubated with different concentrations of cisplatin (0, 10, 20, 40, 80 and 100 µM) at 24 h. Each concentration of cisplatin was tested on 3 wells of the 96-well plates containing 1×10 4 MCF-7cells. In each experiment, three MCF-7 cultured wells with no drug incubation were used as negative controls [10].…”

“…The 50 % lethal concentration (LC 50 ) value of cisplatin on MCF-7 cells at 24 h was calculated. LC 50 was determined by probit analysis using Pharm PCS (Pharmacologic Calculation System) statistical package (Springer Verlag, USA) [10].…”

Silibinin: An Inhibitor of Mir-181a Gene Expression in Sk-Br-3 Breast Cancer Cell Line

“…[21][22][23][24][25] It has been shown to modulate the expression of large numbers of miRNAs, small [22][23][24][25] nucleotides long non-coding RNAs, in cancer cells that lead to reduction of tumor growth. [26][27][28] Also, miRNAs play an important role in the modulation of chemosensitivity of tumor cells.…”

Silibinin reverses epithelial-to-mesenchymal transition in metastatic prostate cancer cells by targeting transcription factors

“…Silibinin possesses strong anticancer efficacy against both androgen-dependent and androgen-independent prostate cancer, wherein it inhibits not only cell growth, but also cell invasion and metastasis (11)(12)(13)(14). In prostate cancer PC-3 cells, silibinin exerts inhibitory effects on cell viability, adhesion and migration (13).…”