Evaluation of shape variability of stallion sperm heads by means of image analysis and Fourier descriptors

Severa, Libor; Máchal, L.; Švábová, Lucia; Mamica, O.

doi:10.1016/j.anireprosci.2009.12.007

Cited by 27 publications

(15 citation statements)

References 30 publications

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“…The largest difference is observed near the sharp end of the eggshell. Another possibility of how to describe the egg contour with a function obtained from non-linear regression, which depends on L and B, is proposed by Severa et al [22] This procedure could be an alternative to the above-mentioned approach.…”

Section: Resultsmentioning

confidence: 98%

Novel Approaches in Mathematical Description of Hen Egg Geometry

Severa

Nedomová

Buchar

et al. 2013

International Journal of Food Properties

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Precise quantification of the profile of a hen egg can provide a powerful tool for analysis of egg shape and following solving of various technological problems. The article presents a new approach of the eggshell geometry determination using and analyzing the egg digital image and edge detection techniques. The detected points on the eggshell contour were fitted by the Fourier series. The obtained equations describing an egg profile were used to calculate the egg volume, surface area, and radius of curvature with a much higher degree of precision in comparison with previously published approaches. It was shown that radii of curvature at the critical points of the egg profile are independent on the egg shape index. The article shows and quantifies the limitations of the common and frequent procedures.

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Section: Resultsmentioning

confidence: 98%

Novel Approaches in Mathematical Description of Hen Egg Geometry

Severa

Nedomová

Buchar

et al. 2013

International Journal of Food Properties

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“…Studies by Saravia et al (2007) have demonstrated that Duroc boars have got larger and more elliptical heads than boars of other breeds. Determining differences is sperm head dimensions facilitates more precise assessment of the boar's spermiogram (Severa et al 2010). Sperm head abnormalities make it possible to distinguish fertile males from boars with decreased fertility (Gravance et al 1996).…”

Section: Discussionmentioning

confidence: 99%

Effect of sperm concentration in an ejaculate on morphometric traits of spermatozoa in Duroc boars

Kondracki¹,

Wysokińska²,

Iwanina³

et al. 2011

Polish Journal of Veterinary Sciences

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The experimental material consisted of 75 ejaculates collected form 8 Duroc boars. The ejaculates were divided into three groups according to sperm concentration in an ejaculate. An ejaculate was obtained from each boar monthly and it was used to make microscopic preparations to examine spermatozoa morphology. In each preparation morphometric measurements were taken of fifteen randomly selected spermatozoa characterized by normal morphology. The following measurements of spermatozoa were taken: length and width of the spermatozoa head, head area, length of the flagellum, perimeter of the spermatozoon head and total spermatozoon length. The results were used to calculate indicators of spermatozoa morphology. Moreover, assessments were made of frequency of morphological defects to isolate spermatozoa with primary and secondary abnormalities following the Blom classification system. It was found that the concentration of spermatozoa in the ejaculate influenced the morphometric characteristics of spermatozoa. Ejaculates with low sperm concentrations are characterized by larger spermatozoa as compared to ejaculates with high sperm concentrations. However, sperm concentration in the ejaculate does not much influence the shape of spermatozoa.

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“…Sperm concentration was evaluated haemocytometrically and the number of sperm was counted in Bürker's chamber. Morphological evaluation of the ejac ulate was conducted on preparations stained using the method of Farelly (SEVERA et al, 2010). In each preparation 200 sperm were evaluated and if they had no defect they were evaluated as "morphologically normal sperm" (PEÑA MARTÍNEZ, 2004).…”

Section: Methodsmentioning

confidence: 99%

The effect of the age of dogs on their ejaculate

Filipčík¹,

Vágenknechtová²,

Hošek³

et al. 2014

Acta Univ. Agric. Silvic. Mendelianae Brun.

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FILIPČÍK, R., VÁGENKNECHTOVÁ, M., HOŠEK, M., JARINKOVIČOVÁ, L.: The eff ect of the age of dogs on their ejaculate. Acta univ. agric. et silvic. Acta univ. agric. et silvic. Mendel. Brun., 2011, LIX, No. 3, pp. 45-50 The eff ect of the age of dogs on quantitative and qualitative parameters of the ejaculate was explored. We evaluated 43 dogs; from each dog we collected three samples on three successive days. The dogs were divided into three age categories: 1.5-2 years; 2-3 years; and 3-5 years. The maximum ejaculate volume was collected from dogs of 2-3 years of age; the average volume of ejaculate from 3 collections was 9.1 ml. Microscopic examinations of the ejaculate of this age category of dogs showed that the highest average sperm concentration was 153.62. . In older dogs the ejaculate volume decreased considerably with increasing frequency of collections (8 ml > 5 ml > 2 ml at the 3 rd collection). The amount of motile sperm was the highest in 3 to 5-year-old dogs (76%) and was related with the highest proportion of morphologically normal sperm (66.8%). However in the 3 rd collection of this age category we saw a signifi cant (P < 0.05) increase in the amount of pathologically changed sperm (29.5 < 28.8 < 41.2%), mostly in the structure of the fl agellum. Signifi cant diff erences in the quantity and quality of the ejaculate were discovered not only among the age categories of the dogs but also among the individual collections. dog ejaculate, volume of ejaculate, sperm activity, sperm concentrationThe number of breeders involved in dog breeding is relatively high. However if the owner of the dog is to be a successful breeder his dog must produce a suffi cient amount of good-quality ejaculate capable of fertilisation. The fertility of dogs can be evaluated in two ways. The breeder may wait to see if the bitch will conceive a er mating or if she gives birth to pups. However, such an approach is unprofessional; for reproduction purposes the breeder should use only dogs whose qualitative and quantitative parameters of the ejaculate had been examined. The birth of pups should be taken as the fi nal confi rmation of the fertility of the dog. DEIBEL et al. (1976), DUNPHY (1989), RIGAU et al. (2001) evaluated the basic qualitative parameters of the canine ejaculate. According to KUTZLER (2005) the optimal room temperature for ejaculate collection is 20 °C; the colour, volume, motility, concentration and morphological structure of the ejaculate should be evaluated. GUNAY et al. (2003) evaluated the sperm fraction of the ejaculate of seven German shepherd dogs. On one day the ejaculate was collected twice within 60 minutes. Between the fi rst and the second collection the authors discovered statistically signifi cant (P < 0.05) diff erences in the volume of the ejaculate and sperm concentration, but not in the motility, live sperm count and amount of morphological changes in the sperm structure. SCHAFER et al. (1997) collected canine ejaculates twice a week over a period of 6 months and they discovered si...

show abstract

Evaluation of shape variability of stallion sperm heads by means of image analysis and Fourier descriptors

Cited by 27 publications

References 30 publications

Novel Approaches in Mathematical Description of Hen Egg Geometry

Novel Approaches in Mathematical Description of Hen Egg Geometry

Effect of sperm concentration in an ejaculate on morphometric traits of spermatozoa in Duroc boars

The effect of the age of dogs on their ejaculate

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