Evaluation of radish (Raphanus sativus L.) peroxidase activity after high-pressure treatment with carbon dioxide

Fricks, Alini Tinoco; Souza, Dayse Pereira Barbosa; Oestreicher, Enrique G.; Antunes, O. A. C.; Girardi, Jonathan S.; Oliveira, Débora de; Dariva, Cláudio

doi:10.1016/j.supflu.2005.11.019

Cited by 42 publications

(37 citation statements)

References 42 publications

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“…Conversely, some works present in the literature show that solvents with low dielectric constant, such as propane, could keep or even enhance enzyme activity and stability (Knez and Habulin 2002;Oliveira et al 2006a;Oliveira et al 2006b;Fricks et al 2006;Andrade et al 2008). Since the solvent properties affect the specific interaction with enzymes, different effects may be obtained depending on the studied enzyme (Kasche et al 1988;Fricks et al 2006).…”

Section: Lyophilized Lipase Amano Psmentioning

confidence: 99%

“…The experiments involving the lyophilized and immobilized lipases were performed in a laboratory-scale unit similar to that employed by Fricks et al (2006), which consists basically of a solvent (propane) reservoir, two thermostatic baths, a syringe pump (ISCO 260D), a stainless steel vessel with an internal volume of 3 ml, and an absolute pressure transducer (Smar, LD301) equipped with a portable programmer (Smar, HT201) with a precision of ±0.37 bar, as schematically presented in Fig. 1.…”

Section: High-pressure Treatment Of Solid Enzymementioning

confidence: 99%

“…According to many authors, supercritical carbon dioxide could remove the essential water responsible for keeping the enzymatic activity (Primo et al 2007;Oliveira et al 2006a;Oliveira et al 2006b;Fricks et al 2006).…”

Section: Lyophilized Lipase Amano Psmentioning

confidence: 99%

“…Since the solvent properties affect the specific interaction with enzymes, different effects may be obtained depending on the studied enzyme (Kasche et al 1988;Fricks et al 2006).…”

Section: Lyophilized Lipase Amano Psmentioning

confidence: 99%

“…In fact, enzyme stability and activity may depend on the enzyme species, the characteristics of the compressed fluid, the water content of the enzyme/support/reaction mixture, and the process variables involved, which means that very distinct effects can be achieved depending on the characteristics of the system under investigation (Chaudhary et al 1996;Kamat et al 1995;Kamat et al 1993;Kamat et al 1992;Taniguchi et al 1987;Tedjo et al 2000;Chen et al 1992;Ishikawa et al 1996;Oliveira et al 2006a;Oliveira et al 2006b;Gieβauf and Gamse 2000;Fricks et al 2006).…”

Section: Introductionmentioning

confidence: 99%

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Effect of Treatment with Compressed Propane on Lipases Hydrolytic Activity

Franken

Marcon

Treichel

et al. 2008

Food Bioprocess Technol

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The objective of this work was to assess the influence of compressed propane treatment on the hydrolytic activity of three lipases (Amano PS, Amano AY30, and a non-commercial lipase from Yarrowia lipolytica) free, resuspended, and in immobilized form. To evaluate the effect of process variables on the lipase activity, a semifactorial experimental design with two levels and four variables was employed for free and immobilized lipases and a full 2 2 experimental design was carried out for lipases in solution. The residual activity was defined as the ratio of lipase activity before and after treatment with pressurized propane. For free and immobilized lipases, an enhancement in residual lipase activity in most of the experimental conditions investigated was observed. In the case of resuspended lipases, it is shown that enzyme kinetics is sensitive to treatment with compressed propane resulting in remarkable gains and losses of enzyme activity. In a general way, the results showed that the enzyme activity changes significantly depending on the enzyme, the presentation form, and the experimental conditions investigated, allowing the selection of operational conditions in terms of temperature, pressure, exposure time, and depressurization rate for advantageous application of these biocatalysts in hydrolysis reactions.

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Section: Lyophilized Lipase Amano Psmentioning

confidence: 99%

Section: High-pressure Treatment Of Solid Enzymementioning

confidence: 99%

Section: Lyophilized Lipase Amano Psmentioning

confidence: 99%

“…Since the solvent properties affect the specific interaction with enzymes, different effects may be obtained depending on the studied enzyme (Kasche et al 1988;Fricks et al 2006).…”

Section: Lyophilized Lipase Amano Psmentioning

confidence: 99%