Effect of Treatment with Compressed Propane on Lipases Hydrolytic Activity

Abstract

The objective of this work was to assess the influence of compressed propane treatment on the hydrolytic activity of three lipases (Amano PS, Amano AY30, and a non-commercial lipase from Yarrowia lipolytica) free, resuspended, and in immobilized form. To evaluate the effect of process variables on the lipase activity, a semifactorial experimental design with two levels and four variables was employed for free and immobilized lipases and a full 2 2 experimental design was carried out for lipases in solution. The residual activity was defined as the ratio of lipase activity before and after treatment with pressurized propane. For free and immobilized lipases, an enhancement in residual lipase activity in most of the experimental conditions investigated was observed. In the case of resuspended lipases, it is shown that enzyme kinetics is sensitive to treatment with compressed propane resulting in remarkable gains and losses of enzyme activity. In a general way, the results showed that the enzyme activity changes significantly depending on the enzyme, the presentation form, and the experimental conditions investigated, allowing the selection of operational conditions in terms of temperature, pressure, exposure time, and depressurization rate for advantageous application of these biocatalysts in hydrolysis reactions.

Overview of Fungal Lipase: A Review

“…The immobilized lipase had improved resistance to thermal denaturation than the native enzyme [154]. Reetz et al [155] have studied an immobilization procedure using alkyl silane precursors of the type R Si (OCH 3 ) 3 and mixtures of R Si(OCH 3 ) 3 and Si(OCH 3 ) 4 to immobilize C. antarctica lipase. Yong et al [156] used the porous hydrophilic magnetic microspheres to immobilized C. rugosa lipase.…”

Extracellular Alkaline Lipase from a Novel Fungus Curvularia sp. DHE 5: Optimization of Physicochemical Parameters, Partial Purification and Characterization

“…Consequently, lipases find application in different industrial sectors, i.e. synthesis of biosurfactants, detergent formulations, oleochemical and agrochemical industries, paper manufacturing and pharmaceutical pro cessing (2). Lipases occur widely in nature and their presence has been reported in several plants, animals and microorganisms (including bacteria, fungi, yeasts and actinomycetes).…”

Isolation and Screening of Lipase-Producing Fungi with Hydrolytic Activity

“…3.1.1.3) can be used in fat and oil modification by reactions of hydrolysis, esterification, and/or interesterification, being important catalysts in the production of specific fatty acids or glycerides from vegetable oils (Kumar et al 2005;Bradoo et al 2002). Several techniques have been developed to obtain higher conversions, as highly specific enzymes for each application, improving the possibility of industrial applications for these biocatalysts (Franken et al 2008;Soccol & Vandenberghe 2003).…”