Evaluation of Probiotic Properties of Novel Brazilian Lactiplantibacillus plantarum Strains

Coelho-Rocha, Nina Dias; Jesus, Luís Cláudio Lima de; Barroso, Fernanda Alvarenga Lima; Silva, Tales Fernando da; Ferreira, Ênio; Gonçalves, José Eduardo; Martins, Fernanda Pereira; Carvalho, Rodrigo Dias de Oliveira; Barh, Debmalya; Azevedo, Vasco

doi:10.1007/s12602-022-09978-6

Cited by 18 publications

(7 citation statements)

References 87 publications

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“…Here, none of the treatments was able to maintain food and liquid intake in face of 5-FU administration. This inability to attenuate the decrease of food and liquid intake has been reported for other probiotic species in this animal model, like Lactobacillus delbrueckii [51,52], Lactiplantibacillus plantarum (formerly Lactobacillus plantarum) [21], and Lacticaseibacillus rhamnosus (formerly Lactobacillus rhamnosus) [42]. Among the four postbiotic formulations tested, only two, heat-inactivated CEC15 (CECi) and EcN cell-free supernatant (EcNs), were able to attenuate weight loss.…”

Section: Discussionsupporting

confidence: 59%

“…More recently we evaluated and compared the protective effect of both strains, EcN and CEC15, in a model of intestinal mucositis induced by the drug 5-Fluorouracil (5-FU) in mice [20]. This model presents high severity in the in ammatory process and allows a better evaluation of probiotics' anti-in ammatory effects [21]. We demonstrated the preventive bene cial issue of CEC15 in this acute in ammatory model [20].…”

Section: Introductionmentioning

confidence: 99%

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Postbiotic effect of Escherichia coli CEC15 and Escherichia coli Nissle 1917 on amurine model of 5-FU-induced intestinal mucositis

Glória,

Silva,

Gomes

et al. 2023

Preprint

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Postbiotics are preparations of inanimate microorganisms and/or their components that confers a health benefit on the host. The Escherichia coli strains Nissle 1917 (EcN) and CEC15 have shown beneficial effects, in their live form, in murine models of intestinal inflammation, such as colitis and mucositis. However, the use of live bacteria, in special by immunocompromised individuals, had raised concerns. Based on this, the present study evaluated the effects as postbiotic of heat-inactivated, and cell-free supernatant preparations of EcN and CEC15 in attenuating 5-fluorouracil (5-FU)-induced intestinal mucositis in mice and compared them with the probiotic effects of the live preparations. BALB/c mice were fed daily, by gavage, with 1010 CFU of live or inactivated bacteria, or with 300 µL of cell-free supernatant for 12 days. On the 10th day, all animals, except for the control group, received an intraperitoneal injection of 5-FU (300 mg/kg). After 72 h of 5-FU administration, animals were euthanized, and the ileum and blood were collected for analysis. Treatments with live and heat-inactivated CEC15 mitigated weight loss, preserved intestinal length, reduced histological damage, maintained goblet cells, decreased neutrophil infiltration, and modulated expression of inflammatory and barrier genes, when compared to 5-FU mucositis controls. EcN showed more limited effects. CEC15 upregulated mRNA expression of the mucin MUC2 and tight junction protein TJP1. Overall, CEC15 demonstrated protective effects against 5-FU-induced mucositis, whatever the way it was administered: live bacteria, heat-inactivated bacteria, or cell-free supernatant. This suggests CEC15 mediates a protective response via secreted metabolites and does not require viability.

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Section: Discussionsupporting

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Postbiotic effect of Escherichia coli CEC15 and Escherichia coli Nissle 1917 on amurine model of 5-FU-induced intestinal mucositis

Glória,

Silva,

Gomes

et al. 2023

Preprint

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“…Therefore, all tested strains were assayed for adhesion on Caco-2 monolayers. The adhesion capacity of CB56 and 11A strains resulted higher than other L. plantarum probiotic candidates previously tested [3,47,66] and in the range of values observed for well-known probiotics [49,63,67,68]. The biofilm-forming capacity of LAB confers protection against hostile environmental conditions and has the potential to delay pathogens' growth [69][70][71], e.g.…”

Section: Discussionmentioning

confidence: 90%

“…Binding the intestinal mucosa is another criterion for selecting probiotics, as it indicates their potential to initiate colonisation and persist in the gut, thereby reinforcing the intestinal barrier. Human enterocyte-like Caco-2 cells are a well-accepted in vitro model for evaluating such property [ 62 , 65 , 66 ]. Therefore, all tested strains were assayed for adhesion on Caco-2 monolayers.…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory Activity on Human Macrophages by Cell-Free Supernatants to Explore the Probiotic and Postbiotic Potential of Lactiplantibacillus plantarum Strains of Plant Origin

Rocchetti

Russo

Simone

et al. 2023

Probiotics & Antimicro. Prot.

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Upon dietary administration, probiotic microorganisms can reach as live cells the human gut, where they interact with the microbiota and host cells, thereby exerting a beneficial impact on host functions, mainly through immune-modulatory activities. Recently, attention has been drawn by postbiotics, i.e. non-viable probiotic microbes, including their metabolic products, which possess biological activities that benefit the host. Lactiplantibacillus plantarum is a bacterial species that comprises recognised probiotic strains. In this study, we investigated in vitro the probiotic (and postbiotic) potential of seven L. plantarum strains, including five newly isolated from plant-related niches. The strains were shown to possess some basic probiotic attributes, including tolerance to the gastrointestinal environment, adhesion to the intestinal epithelium and safety. Besides, their cell-free culture supernatants modulated cytokine patterns in human macrophages in vitro, promoting TNF-α gene transcription and secretion, while attenuating the transcriptional activation and secretion of both TNF-α and IL-8 in response to a pro-inflammatory signal, and enhancing the production of IL-10. Some strains induced a high IL-10/IL-12 ratio that may correlate to an anti-inflammatory capacity in vivo. Overall, the investigated strains are good probiotic candidates, whose postbiotic fraction exhibits immunomodulatory properties that need further in vivo studies. The main novelty of this work consists in the polyphasic characterisation of candidate beneficial L. plantarum strains obtained from relatively atypical plant-associated niches, by an approach that explores both probiotic and postbiotic potentials, in particular studying the effect of microbial culture-conditioned media on cytokine pattern, analysed at both transcriptional and secretion level in human macrophages.

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“…An explanation for the above issues may also be found in the paper by Coelho-Roche et al [37]. They aimed to investigate the probiotic properties and the gastrointestinal protective effects of nine novel L. plantarum strains isolated in Brazil.…”

Section: Lactiplantibacillus Plantarummentioning

confidence: 99%

Looking for the Ideal Probiotic Healing Regime

et al. 2023

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Wound healing is a multi-factorial response to tissue injury, aiming to restore tissue continuity. Numerous recent experimental and clinical studies clearly indicate that probiotics are applied topically to promote the wound-healing process. However, the precise mechanism by which they contribute to healing is not yet clear. Each strain appears to exert a distinctive, even multi-factorial action on different phases of the healing process. Given that a multi-probiotic formula exerts better results than a single strain, the pharmaceutical industry has embarked on a race for the production of a formulation containing a combination of probiotics capable of playing a role in all the phases of the healing process. Hence, the object of this review is to describe what is known to date of the distinctive mechanisms of each of the most studied probiotic strains in order to further facilitate research toward the development of combinations of strains and doses, covering the whole spectrum of healing. Eleven probiotic species have been analyzed, the only criterion of inclusion being a minimum of two published research articles.

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Evaluation of Probiotic Properties of Novel Brazilian Lactiplantibacillus plantarum Strains

Cited by 18 publications

References 87 publications

Postbiotic effect of Escherichia coli CEC15 and Escherichia coli Nissle 1917 on amurine model of 5-FU-induced intestinal mucositis

Postbiotic effect of Escherichia coli CEC15 and Escherichia coli Nissle 1917 on amurine model of 5-FU-induced intestinal mucositis

Immunomodulatory Activity on Human Macrophages by Cell-Free Supernatants to Explore the Probiotic and Postbiotic Potential of Lactiplantibacillus plantarum Strains of Plant Origin

Looking for the Ideal Probiotic Healing Regime

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