Evaluation of plant-mediated Silver nanoparticles synthesis and its application in postharvest Physiology of cut Flowers

Abstract

Today the use of silver nanoparticles is becoming increasingly widespread due to their wide applications as antimicrobial agent. Green synthesis of silver nanoparticles (SNPs) using the petal extract of saffron (Crocus sativus) as a reducing agent from 5 mM AgNO 3 has been investigated in this work. Diverse petal extracts quantities and reaction times were used for the synthesis of SNPs. The resulting SNPs were characterized by means of UV-Vis, XRD and FTIR techniques. SNPs were synthesized rapidly within 30 min of incubation period and synthesized SNPs showed an absorption peak at 380-400 nm in the UV-Vis spectrum. XRD spectrum confirmed the formation of metallic silver, too. Green synthesized SNPs were used as antimicrobial agent against three bacterial genera of Bacillus, Pseudomonas and Acinetobacter which contaminate preservative solution of cutflowers, too. According to the results biosynthesized SNPs using saffron petals successfully controlled these bacteria and have made them promising candidates as new generation of antimicrobials. This route is rapid, simple without any hazardous chemicals and economical to synthesized SNPs.

Current Development of Silver Nanoparticle Preparation, Investigation, and Application in the Field of Medicine

“…With these methods, molecules produced by living organisms such as bacteria, fungi, or plants replace the reducing and capping agents [15][16][17][18]. For example, AgNO 3 is added to chitosan obtained from various species of bacteria and dissolved in acetic acid.…”

Unexplored vegetal green synthesis of silver nanoparticles: A preliminary study with Corchorus olitorus Linn and Ipomea batatas (L.) Lam

“…Silver nanoparticles are receiving great attention due to their applications in different areas such as biotechnology, packaging, electronics, medicine and coatings (Solgi, 2014). The synthesis of nanoparticles using bio-entities *Corresponding author.…”

Evaluation of Piper betle mediated silver nanoparticle in post-harvest physiology in relation to vase life of cut spike of Gladiolus

“…SNPs showed more efficient antimicrobial agent due to their interaction with the bacterial cell membrane (Nabikhan et al 2010). The antimicrobial effect of SNPs is due to structural changes of the bacterial cell membrane, blockage of DNA replication, the debauchery of proton motive force, and finally cell death (Solgi et al 2009;Solgi 2014). Antimicrobial activity of plant-mediated synthesized SNPs reported earlier (Kaviya et al 2011;Maity et al 2014;Solgi 2014;Yugandhar et al 2015).…”

“…The antimicrobial effect of SNPs is due to structural changes of the bacterial cell membrane, blockage of DNA replication, the debauchery of proton motive force, and finally cell death (Solgi et al 2009;Solgi 2014). Antimicrobial activity of plant-mediated synthesized SNPs reported earlier (Kaviya et al 2011;Maity et al 2014;Solgi 2014;Yugandhar et al 2015). Thus, the PbSNPs appeared to be an effective antibacterial agent that significantly reduced the bacterial growth in vase solution as well as vascular blockage in cut spike with its increasing concentration.…”

“…The mode of action of silver nanoparticles to act as antimicrobial agent is not clearly known. The silver nanoparticle synthesis using plant extracts is preferred as this method is non-toxic, cost-effective, eco-friendly, and easy to scale up in contrast to the chemical procedures which use hazardous material causing adverse effects to this approach (Dubey et al 2010;Kaushik et al 2010;Solgi and Taghizadeh 2012;Solgi 2014). Recently, several groups of researchers have synthesized silver nanoparticles using a variety of easily available plants like banana (Bankar et al 2010), aloe (Chandran et al 2006), pomegranate and damask rose (Solgi and Taghizadeh 2012), geranium leaves (Shankar et al 2003), and basil (Ahmad et al 2010).…”