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Background: The purpose of this study is to evaluate the effect of green synthesized silver nanoparticles on postharvest physiology of cut flower in relation to improvement of its vase life as well as keeping quality of the flowers taking gladiolus cut spikes as a model. Results: The synthesized Piper betle mediated silver nanoparticles (PbSNPs) were characterized by using UVvisible spectroscopy and transmission electron microscopy studies. It was revealed that PbSNPs ranges from 30 to 50 nm in diameter and spherical in shape. The experiments were conducted to study the effect of PbSNPs on post-harvest physiology, vascular blockage, prolonging vase life, and keeping the quality of cut spike of gladiolus. The treatment consisted of six vase solutions, viz. distilled water (control), 4% sucrose (T 1 ), 4% sucrose + 100 ppm 5-SSA (T 2 ), 4% sucrose + 2 ppm PbSNPs (T 3 ), 4% sucrose + 4 ppm PbSNPs (T 4 ), and 4% sucrose + 6 ppm PbSNPs (T 5 ). Experimental outcomes depicted that T 4 vase solution gives the best results in all the aspects statistically compared to its positive control (T 2 ). The results also show PbSNPs in vase solution might have played a significant role for scavenging the reactive oxygen species by inducing antioxidant enzyme system at the time of senescence, leading to a decrease in lipid peroxidation and increase in the membrane stability.Conclusions: This study revealed that application of T 4 vase solution helps to maintain spikes fresh and dry weight, reduce the vascular blockage, improve the antioxidative defense, and stabilize the membrane integrity that leads to delay senescence.

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Background: There is insufficient evidence in the literature whether pregnancy with anti-thyroid peroxidase antibody negative subclinical hypothyroidism is benefited with treatment.Methods: The 100 uncomplicated primigravida women before 16 weeks of gestation who were anti thyroid peroxidase antibody negative and diagnosed as subclinical hypothyroid based on serum thyroid stimulating hormone (TSH), Free T4 (FT4) and anti-thyroid peroxidase antibody (anti TPO Ab) were enrolled in this study. They were divided into case and control group having 50 patients in each arm. Case group were treated with levothyroxine therapy as per the recommended dose. Maternal and perinatal outcome were compared between the two groups.Results: In our study we had found increased percentage of cases of antepartum hemorrhage (APH), pregnancy induced hypertension (PIH), pre-labour rupture of membrane (PROM), preterm delivery, meconium-stained liquor, intrauterine growth restriction (IUGR), low birth weight (LBW), APGAR score at 1 and 5 minutes, neonatal hyperbilirubinemia and NICU admission among women who were not treated but it was not statistically significant when compared with control group.Conclusions: When compared between the treated and non-treated group in anti TPO Ab negative subclinical hypothyroid patients, we didn’t find any significant difference in parameters studied by us. In view of inadequate literature, controversy exists whether to treat or not anti TPO negative subclinical hypothyroidism in pregnancy with levothyroxine.