Evaluation of phenolic contents and antioxidant activities of brown seaweeds belonging to Turbinaria spp. (Phaeophyta, Sargassaceae) collected from Gulf of Mannar

Chakraborty, Kajal; Praveen, Nammunayathuputhenkotta Krishnankartha; Vijayan, K K; Rao, Gonugontla Syda

doi:10.1016/s2221-1691(13)60016-7

Cited by 69 publications

(50 citation statements)

References 30 publications

(51 reference statements)

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“…In the present study was aimed to explore the antibacterial, antioxidant and anticancer properties of T. conoides through evaluating cytotoxicity, cell cycle arrest and apoptosis in HepG2 cell line. Brown seaweeds are commercially important renewable resources because which are possessed enormous amount secondary metabolites of potential bioactive compounds such as terpenoid, phlorotanins, phenolic, flavonoids, fucoidans, sterols and glycolipids are extensively documented [11]. Screening of secondary metabolites in the present study was showed that the except resins and alkaloid, all other secondary metabolites were found to be presence in the ethanol extract of T. conoides (Table 1).…”

Section: Discussionmentioning

confidence: 50%

“…In-vitro antioxidant activity measured by single assay is reflects the chemical reactivity under the specific conditions. Therefore, it is necessary to assay the solvent fractions through various methods revealed an overall idea about their broad spectrum of antioxidant activity [11]. The principal of assays based on quantification and radical scavenging property by different mechanisms viz., donating hydrogen molecule, electron transfer, reducing ability and metal ion chelating ability also considered.…”

Section: Discussionmentioning

confidence: 99%

“…In which, cyclohexane extract was possessed a broad array of antibacterial activity and exhibited remarkable antifungal property over the other extracts [6]. Turbinaria spp such as T. conoides and T. ornata collected from Gulf of Mannar of Southeastern coast of India were also reported to possess antioxidant activity in ethyl acetate fractions due to the presence of total phenolic contents [11]. Hence, several literatures emphasized that the potential implication of marine products from brown seaweeds exhibited antitumour activity.…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial, antioxidant and anticancer properties of Turbinaria conoides (J. Agardh) Kuetz

et al. 2017

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Background: Cancer causes leading death in the world population due to exposure of various carcinogenic/mutagenic agents, radiation and life style. There are 2.6 million new cases diagnosed each year. Therefore, the objective is aimed to study the antibacterial and antioxidant activities of solvent fractions of ethanolic extract of T. conoides and its anticancer activity also evaluated by analyzing cytotoxicity, cell cycle arrest and apoptosis in HepG2 cell line. Methods: Antibacterial activity was done by disc diffusion method and expressed as in millimeter diameter of zone inhibition. Antioxidant activity was done by ABTS radical assay, superoxide radical assay, iron chelation and uric acid formation inhibitory assays. The cytotoxicity efficacy was estimated using MTT assay. Annexin-V FITC kit was used to estimate the apoptosis and cell cycle arrest by flowcytometer. Morphological changes of cell through alteration of nuclear content and mitochondrial membrane potential were also examined using Hoechst and JC1 stains under fluorescence microscopy, respectively. Results: Highest antibacterial activity, TAA and RAA were found in EAF followed by DMF, HF and AF. Cytotoxicity of EAF was found to be 67% at 24 h and 83% at 48 h over the standard of quercetin (86% at 48 h). The cancer cells were found to be significantly (p < 0.05) higher in the proliferative G0/G1 phase where as significantly decreased in the S phase. Hence, treatment with T. conoides fraction showed statistically (p < 0.05) significant increase of apoptotic cells than that of quercetin standard (32%, 80 μg/mL). The apoptotic cell formation might be due to the change of nuclear content and mitochondrial membrane potential were further confirmed in HepG2 cells under fluorescence microscopy. Conclusion: Ethyl acetate fraction of T. conoides showed highest antibacterial, antioxidant and anticancer activity through exhibiting synergistic effects over the respective standard compounds.

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Section: Discussionmentioning

confidence: 50%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antibacterial, antioxidant and anticancer properties of Turbinaria conoides (J. Agardh) Kuetz

et al. 2017

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“…The TBARS formation inhibitory assay was performed by earlier described method (Chakraborty et al 2013) with suitable modifications. The model system used for this assay was lyophilized green mussel (Perna viridis L.) as a lipid source.…”

Section: Hydrogen Peroxide (H 2 O 2 ) Scavenging Activitymentioning

confidence: 99%

“…DNA, cell membranes, proteins and other cellular constituents are target site of the degradation processes, and consequently induce different kinds of serious human diseases including viz., chronic inflammation, atherosclerosis, cancer, cardiovascular disorders, and ageing (Ruberto et al 2001). Antioxidant compounds play an important role against these diseases, which explains their considerable commercial potential in medicine, food production and the cosmetic industry (Chakraborty et al 2013). Therefore, consumption, and addition of antioxidant in food materials protects the body as well as foods against these degradation processes.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant activities and phenolic contents of three red seaweeds (Division: Rhodophyta) harvested from the Gulf of Mannar of Peninsular India

2013

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The antioxidant activities of methanol extract and its solvent fractions (n -hexane, dichloromethane and ethyl acetate) of three red seaweeds (Hypnea musciformis , H. valentiae , and Jania rubens ) collected from the Gulf of Mannar of South eastern coast of India were evaluated, using different in vitro systems, viz., DPPH, ABTS, HO radical scavenging activities, H 2 O 2 scavenging ability, Fe 2+ ion chelating ability and reducing potential. Folin-Ciocalteu method was used to determine the total phenolic content of the extracts/fractions, and the results were expressed as mg of gallic acid equivalent (GAE)/g of the seaweed extracts/ fractions. Thiobarbituric acid-reactive substances (TBARS) inhibition assay was employed to assess the ability of the seaweed extracts/fractions to inhibit lipid oxidation. Ethyl acetate (EtOAc) fractions of H. musciformis exhibited significantly higher total phenolic content (205.5 mg GAE/g), DPPH· scavenging activity (IC 50 0.6 mg/mL), ABTS .+ scavenging activity (IC 50 0.51 μg/mL), Fe 2+ chelating ability (IC 50 0.70 mg/mL), H 2 O 2 scavenging activity (IC 50 0.39 mg/mL), reducing ability (Abs 700 nm 1.46) and lipid peroxidation inhibitory ability (2.71 MDAEC/kg) (P <0.05) compared to its n-hexane, DCM fractions, crude MeOH extract and MeOH extracts/fractions of H. valentiae and J. rubens. DCM fraction of J. rubens showed significantly higher hydroxyl radical scavenging activity (IC 50 0.55 mg/mL) compared with H. musciformis and H. valentiae (P <0.05). This study indicated the potential use of red seaweeds, in particular, H. musciformis as candidate species to be used as food supplement for increasing the shelf-life of food industry, and candidates in combating carcinogenesis and inflammatory diseases.

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Anti-stress activity of Sargassum polycystum extracts using a cold restraint stress model

Lailatussifa

Husni

Nugroho

2016

Food Sci Biotechnol

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Anti-stress effects of polyphenol extracts of were evaluated. Polyphenol extracts of and diazepam were compared for anti-stress activities using a cold restraint animal stress model. extracts were administered orally at dosages of 150 and 450 mg/kg. Diazepam, an anti-stress agent, was used as a standard drug at 0.18 mg/kg p.o. Both dosages of extracts showed good anti-stress effects. Due to cold restraint stress there was an imbalance in levels of biochemical parameters, including glucose, triglycerides, cholesterol, alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate amino transferase (AST), which were near normalized following adminstration of extracts. Polyphenol extracts of S. polycystum at oral dosages of 150 and 450 mg/kg exerted anti-stress effects.

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Evaluation of phenolic contents and antioxidant activities of brown seaweeds belonging to Turbinaria spp. (Phaeophyta, Sargassaceae) collected from Gulf of Mannar

Cited by 69 publications

References 30 publications

Antibacterial, antioxidant and anticancer properties of Turbinaria conoides (J. Agardh) Kuetz

Antibacterial, antioxidant and anticancer properties of Turbinaria conoides (J. Agardh) Kuetz

Antioxidant activities and phenolic contents of three red seaweeds (Division: Rhodophyta) harvested from the Gulf of Mannar of Peninsular India

Anti-stress activity of Sargassum polycystum extracts using a cold restraint stress model

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