Antioxidant activities and phenolic contents of three red seaweeds (Division: Rhodophyta) harvested from the Gulf of Mannar of Peninsular India

Abstract

The antioxidant activities of methanol extract and its solvent fractions (n -hexane, dichloromethane and ethyl acetate) of three red seaweeds (Hypnea musciformis , H. valentiae , and Jania rubens ) collected from the Gulf of Mannar of South eastern coast of India were evaluated, using different in vitro systems, viz., DPPH, ABTS, HO radical scavenging activities, H 2 O 2 scavenging ability, Fe 2+ ion chelating ability and reducing potential. Folin-Ciocalteu method was used to determine the total phenolic content of the extracts/fractions, and the results were expressed as mg of gallic acid equivalent (GAE)/g of the seaweed extracts/ fractions. Thiobarbituric acid-reactive substances (TBARS) inhibition assay was employed to assess the ability of the seaweed extracts/fractions to inhibit lipid oxidation. Ethyl acetate (EtOAc) fractions of H. musciformis exhibited significantly higher total phenolic content (205.5 mg GAE/g), DPPH· scavenging activity (IC 50 0.6 mg/mL), ABTS .+ scavenging activity (IC 50 0.51 μg/mL), Fe 2+ chelating ability (IC 50 0.70 mg/mL), H 2 O 2 scavenging activity (IC 50 0.39 mg/mL), reducing ability (Abs 700 nm 1.46) and lipid peroxidation inhibitory ability (2.71 MDAEC/kg) (P <0.05) compared to its n-hexane, DCM fractions, crude MeOH extract and MeOH extracts/fractions of H. valentiae and J. rubens. DCM fraction of J. rubens showed significantly higher hydroxyl radical scavenging activity (IC 50 0.55 mg/mL) compared with H. musciformis and H. valentiae (P <0.05). This study indicated the potential use of red seaweeds, in particular, H. musciformis as candidate species to be used as food supplement for increasing the shelf-life of food industry, and candidates in combating carcinogenesis and inflammatory diseases.

Phytochemical Composition and Antioxidant Activity of Edible Red Alga H ypnea musciformis from Bangladesh

“…In addition, methanol extract showed the presence of terpenoid which may for the increased antioxidant activity be compared to other extracts. Similar findings were also reported by Chang and Lin, , whereas Chakraborty et al () reported that methanol extract and its ethyl acetate fractions of Indian originated H. musciformis showed higher antioxidant activity compared to the present study. These differences might be due to the origin of seaweed and the choice of solvent fractions.…”

“… and Chakraborty et al . ). However, Bangladeshi seaweed as a source of antioxidant has not been determined and reports on antioxidant and DNA protection activities of H. musciformis are limited.…”

Antioxidant screening and phenolic content of ethanol extracts of selected Baja California Peninsula macroalgae

“…Although Acanthophora spicifera had low phenolic content, its extract had significant antioxidant activity. This is likely because this species belongs to the red algae family Rhodomelaceae that is rich in bromophenols, sharing one or several benzene rings, where the hydroxyl groups are substituted by a bromine group with potent antioxidant activities (Liu et al 2011;Chakraborty et al 2015). Other abundant compounds in A. spicifera are sulfated polysaccharides such as agaran, which possibly were co-extracted and might also support its antioxidant activity (Ganesan et al 2008).…”

Protective role of marine macroalgae extracts against STZ induced diabetic rats

“…Hence, FM4 was purified and characterized by marine algae. This is due to the presence of oligosaccharides, glycolipids, phenolic compounds and small molecular weight bioactive compounds and their effect was proved in experimental animals [13,14]. Marine algae produce a wide range of new secondary metabolites with various biological activities which may have potential applicability in the field of pharmaceutical industry to provide a stable platform for medicine industries [15].…”