Evaluation of Oxidative Stress in Type 2 Diabetes Mellitus and Follow-up Along with Vitamin E Supplementation

Abstract

Increased oxidative stress is a widely accepted participant in the development and progression of diabetes and its complications. The present study has been undertaken to evaluate oxidative stress in type 2 diabetes mellitus and effect of vitamin E supplementation on oxidative stress. In all 120 subjects were enrolled in the present study, 40 subjects are age and sex matched controls. Test group comprised of clinically diagnosed (n = 80) type 2 diabetic patients. Biochemical parameters like serum MDA, nitric oxide, superoxide dismutase, erythrocyte reduced glutathione and platelet aggregation were analyzed in control and diabetic group. Test group is further categorized as Group I (n = 40) diabetics were treated by only hypoglycemic drugs and Group II (n = 40) diabetics were treated by hypoglycemic drugs with vitamin E supplementation. All above biochemical parameters were again reassessed after 3 months follow-up in both group and its values were compared with its respective baseline levels. The study shows, reduction of oxidative stress, improvement in antioxidant enzymes and endothelial dysfunction in group II, those were on treatment of hypoglycemic drugs along with vitamin E supplementation. Hence the present study may conclude that vitamin E supplementation along with hypoglycemic drugs may be beneficial to type 2 DM patients to minimize vascular complications.

Evaluation of Oxidative Stress in Type 2 Diabetes Mellitus Patients

“…In this study the lipid peroxidation product, MDA measured as TBARS is significantly increased in type 2 diabetics than in controls. This finding is in accordance with findings of other studies as illustrated in Table: 5 [20], [21], [22], [23], [24], [25], [26], [27], [28]. The present study showed significantly increased ceruloplasmin levels in cases when compared to controls.…”

Dietary Supplementation During Diabetes Therapy and the Potential Risk of Interactions

“…Shinde et al [22] demonstrated a reduction in oxidative stress and an improvement in antioxidant enzyme activity and vascular endothelial function in T2DM patients treated with anti-diabetic agents supplemented with vitamin E, as compared with patients treated only with glucose--lowering drugs. However, the pro-oxidative action of vitamin E, which depends on the dose, the duration of supplementation and the duration of diabetes, may alter the expected effect of supplementation [22]. In the present study, the participants' dietary intake of vitamin E and C were rather inadequate, therefore supplementation with these antioxidants would be probably favorable.…”

“…In turn, vitamin E supplementation by T2DM patients can help to reduce the risk of developing cardiovascular disease by preventing the oxidation of low-density lipoproteins [15]. Shinde et al [22] demonstrated a reduction in oxidative stress and an improvement in antioxidant enzyme activity and vascular endothelial function in T2DM patients treated with anti-diabetic agents supplemented with vitamin E, as compared with patients treated only with glucose--lowering drugs. However, the pro-oxidative action of vitamin E, which depends on the dose, the duration of supplementation and the duration of diabetes, may alter the expected effect of supplementation [22].…”

Behavior of the total antioxidant status in a group of subjects with metabolic syndrome

“…At the same time, this finding needs further investigation thus, up to now, many research have regarded the use of the antioxidant supplementation in type 2 diabetic subjects with conflicting results. In fact, several studies have demonstrated that in diabetic subjects vitamin E supplementation improves blood glucose levels [45], and oxidative status parameters [46]. As well as the combination of vitamin E and C reduced HbA1c and increased the activity of some antioxidant enzymes [47].…”