Evaluation of native Trichoderma isolates for the management of sugarcane smut (Ustilago scitaminea) in sugar plantations of Ethiopia

Abstract

Biological control of sugarcane smut (Ustilago scitaminea Syd.) using Trichoderma isolates is one of the untapped potentials in Ethiopian sugarcane plantations. To this end, this study was carried out with the objective to identify potential native Trichoderma isolates for the management of sugarcane smut. A total of 120 soil samples were collected from Kesem, Metehara, and Wonji sugarcane plantation fields. Consequently, 21 Trichoderma isolates were recovered from the samples, and in-vitro antagonist study of Trichoderma isolates against U. scitaminea was done using dual culture technique on fresh PDA. Among the native isolates, 12 of them revealed a pathogen suppression potential of >90% at 7 days after inoculation and most of them had higher pathogen suppression than the commercial isolate. However, five of the isolates were selected as potential candidates based on distinct suppression features, growth rate, and antagonistic performance. Identification of the five effective Trichoderma isolates (WI4-27, MKI2-k17, MI3-m33, WI12-25, and MKI3) was done based on macroscopic and

Factors affecting the production of sugarcane yield and sucrose accumulation: suggested potential biological solutions

“…Hence, Pseudomonas aeruginosa B18 has an important role in plant growth and biological control mechanisms in sugarcane ( Singh et al., 2021a ). Furthermore, many studies have reported that biocontrol agents, such as Bacillus , Pseudomonas fluorescens , and Trichoderma spp., can inhibit the growth of Sporisorium scitamineum ( Tegene et al., 2021 ; Wang et al., 2021 ; Rees et al., 2022 ; Vijitrpanth et al., 2023 ). Most bacteria, including the denitrification-active actinomycete Marmoricola , Iamia , and Reyranella , are helpful to smut resistance ( Duan et al., 2023 ).…”

Prospecting the roles of Trichoderma in sustainable crop production: biotechnological developments and future prospects

“…Gambar 1. Bentuk morfologi spesies Trichoderma: a. Koloni T. asperellum pada media PDA (Dokumentasi pribadi Febri Doni), b. Koloni T. asperellum pada media Rose Bengal Agar (RBA) (Dokumentasi pribadi Febri Doni), c. T. harzianum dibawah mikroskop pada pembesaran 40x (Akbar et al, 2022), jurnal open akses, izin penggunaan gambar tidak diperlukan), d. Klamidospora Trichoderma pada pembesaran 400x (Tegene et al, 2021), jurnal open akses, izin penggunaan gambar tidak diperlukan), e. T. asperellum dibawah scanning mikroskop elektron pada pembesaran 10.000x (de Padua & dela Cruz, 2021), jurnal open akses, izin penggunaan gambar tidak diperlukan).…”

Morphological characterization and biocontrol potential of Trichoderma species isolated from semi-arid soils

“…Este género es utilizado para desarrollar bioproductos comerciales para el control de enfermedades en las plantas (Singh y Jadon, 2019;Carrillo et al, 2020); sin embargo, existen casos de baja eficiencia en el control de fitopatógenos, debido a que las diversas especies de Trichoderma que componen los bioproductos comerciales son originarios de regiones edafoclimáticas diferentes a la zona geográfica donde se aplica (Harman et al, 2010). Por lo tanto, es importante la selección de microorganismos nativos adaptados a las condiciones edafoclimáticas de la región donde se pretende realizar el biocontrol de las enfermedades en plantas (Al-Mekhlafi et al, 2019;Tegene et al, 2021). Alrededor del mundo, se desarrollan actividades agrícolas bajo climas desérticos con limitadas precipitaciones durante el año y temperaturas extremas con más de 40 °C.…”

“…However, there are cases of low efficiency in the control of phytopathogens, since the diverse species of Trichoderma that make up the commercial bioproducts are originally from different edaphoclimatic regions to the geographic area in which it is applied (Harman et al, 2010). It is therefore important to select native microorganisms adapted to the edaphoclimatic conditions of the place in which the biocontrol of plant diseases is intended to be carried out (Al-Mekhlafi et al, 2019;Tegene et al, 2021). Worldwide, agricultural activities are carried in deserts with limited yearly rainfall and extreme temperatures of over 40 °C.…”