Evaluation of Mycobacterium tuberculosis 16 and 71 Heat Shock Proteins in the Cerebrospinal Fluid of Tuberculous Meningitis Patients

Shekhawat, Seema D.

doi:10.9734/bjmmr/2012/1002

Cited by 3 publications

(3 citation statements)

References 30 publications

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“…Our results showed that the levels of host Hsp25, Hsp60, Hsp70, and Hsp90 and MTB Hsp16 were significantly increased in the TB group compared to the high-risk exposure, lowrisk exposure and healthy control groups, which is consistent with our previous reports [14,21,22]. However, an even more interesting finding of this study is the increased levels of the same panel of Hsp(s), which are considered to be ''danger signals,'' in the sera of the high-risk exposure group compared to the low-risk exposure and healthy control groups.…”

Section: Discussionsupporting

confidence: 93%

“…Thus, the host Hsp(s) should be increased in the high-risk exposure group, where the chances of disease are the greatest compared to the low-risk exposure group and healthy controls. It is well known that bacterial Hsp(s) are highly immunogenic and capable of inducing antibody production and T cell activation [14,21,30]. MTB Hsp16 is required for the survival of bacteria in the stationary phase, and therefore, the maintenance of a constant cellular level of this protein is an important strategy for MTB to survive in the dormant state [35].…”

Section: Parameters [Mean With Sd] Tb (N = 10)mentioning

confidence: 99%

“…They function as chaperones and co-chaperones, binding intracellular polypeptide chains and misfolded proteins, preventing aggregation and supporting folding and transport [20]. Our earlier findings suggest that Hsp65 and Hsp71 can be used for the diagnosis of TB [14,21]. We have also reported that Hsp16 is more specific to latency and can be used as a diagnostic marker for LTBI [22].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of heat shock proteins for discriminating between latent tuberculosis infection and active tuberculosis: A preliminary report

Shekhawat

Purohit

Taori

et al. 2016

Journal of Infection and Public Health

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The diagnosis of a latent tuberculosis infection (LTBI) is of the utmost concern. The available tests, the tuberculin skin test (TST) and the Quantiferon-TB Gold test (QFT-G) cannot discriminate between active TB and LTBI. Therefore, the aim of the study is to identify new biomarkers that can discriminate between active TB and LTBI and can also assess the risk of the individual developing active TB. In total, 55 blood samples were collected, of which 10 samples were from the active TB infection group, 10 were from the high-risk exposure group, 23 were from the low-risk exposure group, and 12 were from healthy controls living in a non-TB endemic area. A panel of heat shock proteins (Hsps), including host Hsp25, Hsp60, Hsp70, and Hsp90 and Mycobacterium tuberculosis (MTB) Hsp16, were evaluated in all of the collected samples using ELISA. The levels of the host Hsp(s) (Hsp25, Hsp60, Hsp70 and Hsp90) and MTB Hsp16 were significantly (p<0.05) elevated in the active TB group compared to the high-risk exposure group, the low-risk exposure group and the control group. Notably, the levels of the same panel of Hsp(s) were elevated in the high-risk exposure group compared to the low-risk exposure group. On follow-up, out of the 10 high-risk exposure participants, 3 converted into active TB, indicating that this group has the highest risk of developing TB. Thus, the evaluated panel of Hsp(s) can discriminate between LTBI and active TB. They can also identify individuals who are at the highest risk of developing active TB. Because they can be rapidly detected, Hsp(s) have an edge over the existing diagnostic tools for LTBI. The evaluation of these proteins will be useful in designing better diagnostic methods for LTBI.

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Section: Discussionsupporting

confidence: 93%