Evaluation of host Hsp(s) as potential biomarkers for the diagnosis of tuberculous meningitis

Shekhawat, Seema D.; Purohit, Hemant J.; Taori, Girdhar M.; Daginawala, Hatim F.; Kashyap, Rajpal S.

doi:10.1016/j.clineuro.2015.11.008

Cited by 10 publications

(5 citation statements)

References 27 publications

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“…Host-derived tests CSF adenosine deaminase has shown sensitivities ranging from 50 to 100% and specificity from 63 to 99%, further, adenosine deaminase has difficulty distinguishing TBM from bacterial meningitis [47,97,[100][101][102]. CSF adenosine deaminase cannot be used alone to rule out TBM [97,102,103].…”

Section: Tuberculosis Meningitismentioning

confidence: 99%

“…Delta-like 1 ligand, fetuin and vitamin D binding protein are other immune biomarkers that have been considered for TBM, yet have shown limited utility in an African HIV-infected population [104]. Heat shock proteins found at higher levels in CSF in patients with TBM compared with non-TBM patients and so have been theorized to be a biomarker for TBM -their role as a potential diagnostic test is not clear [100]. Though no immune markers have proven to be adequate diagnostic tests for TBM thus far, given the inadequate of performance of any one test to detect TB bacilli to date in CSF, they may play a role as adjunctive tests in the future [103].…”

Section: Tuberculosis Meningitismentioning

confidence: 99%

“…Other TB meningitis testing Detection of TB lipoarabinomannan (LAM) in the CSF has been explored as a potential diagnostic technique for TBM [91,94,98,100,120]. Patel et al tested CSF for LAM antigen and found a sensitivity of 64% and specificity of 69% for TBM [120].…”

Section: Nucleic Acid Amplification Testsmentioning

confidence: 99%

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Methods for Rapid Diagnosis of Meningitis Etiology in Adults

2020

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Infectious meningitis can be caused by viral, bacterial or fungal pathogens. Despite widely available treatments, many types of infectious meningitis are still associated with significant morbidity and mortality. Delay in diagnosis contributes to poor outcomes. Cerebrospinal fluid cultures have been used traditionally but are time intensive and sensitivity is decreased by empiric treatment prior to culture. More rapid techniques such as the cryptococcal lateral flow assay (IMMY), GeneXpert MTB/Rif Ultra (Cepheid) and FilmArray multiplex-PCR (Biofire) are three examples that have drastically changed meningitis diagnostics. This review will discuss a holistic approach to diagnosing bacterial, mycobacterial, viral and fungal meningitis.

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Section: Tuberculosis Meningitismentioning

confidence: 99%

Section: Tuberculosis Meningitismentioning

confidence: 99%

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Methods for Rapid Diagnosis of Meningitis Etiology in Adults

2020

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“…Hsp27 is a biomarker of infections and also a therapeutic target in cancer [24]. A study showed that the host Hsps such as Hsp25, Hsp60, Hsp70 and Hsp90 were used as potential biomarkers for the diagnosis of tuberculous meningitis (TBM) [25]. Another study indicated that the serum level of Hsp27 as a potential marker was increased in Egyptians with Type 2 Diabetes [26].…”

Section: Introductionmentioning

confidence: 99%

Significance of serum antibodies against HPV E7, Hsp27, Hsp20 and Hp91 in Iranian HPV-exposed women

et al. 2019

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BackgroundAmong different types of human papillomavirus (HPV), types 16 and 18 were known to be high-risk agents causing mainly cervical cancer. Up to now, the potential of HPV E7 protein has been proved as a diagnostic marker of cervical cancer. Moreover, the levels of anti-heat shock protein (Hsp) and anti-high mobility group box-1 (HMGB1) antibodies in cancer patients have been useful in tumor diagnosis. The goal of the present study was to determine the efficiency of the potential serologic markers including HPV E7, Hsp20, Hsp27 proteins and Hp91 peptide in Iranian HPV-exposed women, for the first time.MethodsAt first, the recombinant HPV E7, Hsp20 and Hsp27 proteins were expressed in E. coli system, and purified by affinity chromatography under native conditions. Then, antibody responses were detected against the recombinant proteins as well as Hp91 peptide as potential markers in 49 Iranian women who were seropositive for HPV-16 and 18 L1 capsids (i.e., HPV-exposed women) and 49 controls using indirect ELISA.ResultsOur data indicated that the seroreactivities of women exposed to HPV16, HPV18 and both of them against the recombinant E7, Hsp20, Hsp27 proteins and Hp91 peptide were significantly higher than those in control group (p < 0.05 for HPV16 or HPV18; p < 0.01 for both of them versus all markers). HPV-exposed women with high antibody responses to HPV-16 and 18 L1 capsids as a commercial biomarker had significant seroreactivity to HPV-16 and 18 E7 and Hsp27 (p < 0.05). The recombinant E7 and Hsp27 proteins showed higher efficiency than Hsp20 and Hp91 for detection of individuals exposed to HPV infections (p < 0.05).ConclusionGenerally, the levels of serum E7 and Hsp27 were increased in HPV-16 and 18 L1- seropositive women suggesting their potential value as a diagnostic marker for HPV infections.Electronic supplementary materialThe online version of this article (10.1186/s12879-019-3780-2) contains supplementary material, which is available to authorized users.

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“…Оксидативный стресс сопровождается усиленным синтезом sHsp -они активируют или стабилизируют глюкозо-6-фосфат дегидрогеназу, фермент, продуктом которого является НАДН. Повышение уровня НАДНДГ увеличивает активность глутатион-редуктазы, что обеспечивает поддержание нормального уровня вос-становленного глутатиона, фактора антиоксидантной защиты [11,22,28].…”

Section: таблица белки теплового шока в эукариотических клеткахunclassified

Heat Shock Proteins. Properties. Role in Adaptation. Methodological Approaches to Definition

Максимович

Бонь

2020

BIOMEDICINE

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The aim of this review article is to generalize and systematize literature data on the properties of heat shock proteins, as well as their role in adaptation processes and experimental methods of their investigation. The synthesis of heat shock proteins is a universal response to stress, which plays an important role in protecting cells from negative external impacts. Heat shock proteins participate in fundamental cellular processes. Altered expression of heat shock proteins can serve as an important diagnostic marker of cellular responses to damage.

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Evaluation of host Hsp(s) as potential biomarkers for the diagnosis of tuberculous meningitis

Cited by 10 publications

References 27 publications

Methods for Rapid Diagnosis of Meningitis Etiology in Adults

Methods for Rapid Diagnosis of Meningitis Etiology in Adults

Significance of serum antibodies against HPV E7, Hsp27, Hsp20 and Hp91 in Iranian HPV-exposed women

Heat Shock Proteins. Properties. Role in Adaptation. Methodological Approaches to Definition

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